PMID- 36325297
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221104
IS  - 2049-9469 (Electronic)
IS  - 2049-9450 (Print)
IS  - 2049-9450 (Linking)
VI  - 17
IP  - 5
DP  - 2022 Nov
TI  - Dendritic cells transfected with a polyepitope DNA construct stimulate an 
      antitumor cytotoxic response in various tumors.
PG  - 155
LID - 10.3892/mco.2022.2588 [doi]
LID - 155
AB  - Dendritic cells (DCs) loaded with tumor-associated antigens (TAAs) are known to 
      be crucial for the antitumor response and are still included in various treatment 
      regimens in cancer immunotherapy research. In the present study, a cell-based 
      protocol was evaluated, involving the use of original DNA constructs encoding the 
      wide range of TAA epitopes expressed on different epithelial cancers. The 
      constructs were transfected into in vitro-generated DCs of patients with various 
      types of cancer, including breast, colorectal and non-small cell lung cancer. The 
      direct cytotoxicity assay of effector cells, activated with the transfected DCs, 
      revealed a significant increase in cytotoxicity against autologous tumor cells. 
      The use of DNA constructs encoding a large number of TAAs for insertion into DCs 
      in vitro, aiming to activate a T-cell response may prove to be a reliable and 
      unified approach for immunotherapy and for the prevention of relapse in patients 
      with epithelial cancers.
CI  - Copyright (c) 2020, Spandidos Publications.
FAU - Kurilin, Vasily
AU  - Kurilin V
AD  - Federal State Budgetary Scientific Institution 'Research Institute of Fundamental 
      and Clinical Immunology', Novosibirsk 630099, Russian Federation.
FAU - Kulikova, Ekaterina
AU  - Kulikova E
AD  - Federal State Budgetary Scientific Institution 'Research Institute of Fundamental 
      and Clinical Immunology', Novosibirsk 630099, Russian Federation.
FAU - Shevchenko, Julia
AU  - Shevchenko J
AD  - Federal State Budgetary Scientific Institution 'Research Institute of Fundamental 
      and Clinical Immunology', Novosibirsk 630099, Russian Federation.
FAU - Lopatnikova, Julia
AU  - Lopatnikova J
AD  - Federal State Budgetary Scientific Institution 'Research Institute of Fundamental 
      and Clinical Immunology', Novosibirsk 630099, Russian Federation.
FAU - Obleukhova, Irina
AU  - Obleukhova I
AD  - Federal State Budgetary Scientific Institution 'Research Institute of Fundamental 
      and Clinical Immunology', Novosibirsk 630099, Russian Federation.
FAU - Khantakova, Julia
AU  - Khantakova J
AD  - Federal State Budgetary Scientific Institution 'Research Institute of Fundamental 
      and Clinical Immunology', Novosibirsk 630099, Russian Federation.
FAU - Maksyutov, Amir
AU  - Maksyutov A
AD  - Federal State Budgetary Scientific Institution 'Research Institute of Fundamental 
      and Clinical Immunology', Novosibirsk 630099, Russian Federation.
AD  - State Research Center of Virology and Biotechnology 'Vector', Koltsovo 630559, 
      Russian Federation.
FAU - Kuznetsova, Maria
AU  - Kuznetsova M
AD  - Federal State Budgetary Scientific Institution 'Research Institute of Fundamental 
      and Clinical Immunology', Novosibirsk 630099, Russian Federation.
FAU - Khristin, Alexander
AU  - Khristin A
AD  - State Clinical Hospital No. 1, Novosibirsk, Novosibirsk 630075, Russian 
      Federation.
FAU - Kiryshina, Natalya
AU  - Kiryshina N
AD  - Novosibirsk Regional Clinical Oncological Center, Novosibirsk, Novosibirsk 
      630108, Russian Federation.
FAU - Kozlov, Vadim
AU  - Kozlov V
AD  - Novosibirsk Regional Clinical Oncological Center, Novosibirsk, Novosibirsk 
      630108, Russian Federation.
FAU - Sidorov, Sergey
AU  - Sidorov S
AD  - State Clinical Hospital No. 1, Novosibirsk, Novosibirsk 630075, Russian 
      Federation.
FAU - Sokolov, Andrey
AU  - Sokolov A
AD  - State Clinical Hospital No. 1, Novosibirsk, Novosibirsk 630075, Russian 
      Federation.
FAU - Vitsin, Alexander
AU  - Vitsin A
AD  - State Clinical Hospital No. 1, Novosibirsk, Novosibirsk 630075, Russian 
      Federation.
FAU - Shiku, Hiroshi
AU  - Shiku H
AD  - Federal State Budgetary Scientific Institution 'Research Institute of Fundamental 
      and Clinical Immunology', Novosibirsk 630099, Russian Federation.
AD  - Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan.
FAU - Sennikov, Sergey
AU  - Sennikov S
AD  - Federal State Budgetary Scientific Institution 'Research Institute of Fundamental 
      and Clinical Immunology', Novosibirsk 630099, Russian Federation.
LA  - eng
PT  - Journal Article
DEP - 20221003
PL  - England
TA  - Mol Clin Oncol
JT  - Molecular and clinical oncology
JID - 101613422
PMC - PMC9618840
OTO - NOTNLM
OT  - T-cells
OT  - cytotoxicity
OT  - dendritic cells
OT  - epithelial cancer
OT  - polyepitope DNA construct
COIS- The authors declare that they have no competing interests.
EDAT- 2022/11/04 06:00
MHDA- 2022/11/04 06:01
PMCR- 2022/10/03
CRDT- 2022/11/03 02:44
PHST- 2022/05/13 00:00 [received]
PHST- 2022/09/14 00:00 [accepted]
PHST- 2022/11/03 02:44 [entrez]
PHST- 2022/11/04 06:00 [pubmed]
PHST- 2022/11/04 06:01 [medline]
PHST- 2022/10/03 00:00 [pmc-release]
AID - MCO-17-5-02588 [pii]
AID - 10.3892/mco.2022.2588 [doi]
PST - epublish
SO  - Mol Clin Oncol. 2022 Oct 3;17(5):155. doi: 10.3892/mco.2022.2588. eCollection 
      2022 Nov.