PMID- 36327196
OWN - NLM
STAT- MEDLINE
DCOM- 20221124
LR  - 20230106
IS  - 1530-6992 (Electronic)
IS  - 1530-6984 (Linking)
VI  - 22
IP  - 22
DP  - 2022 Nov 23
TI  - Accelerating Neurite Growth and Directing Neuronal Connections Constrained by 3D 
      Porous Microtubes.
PG  - 8991-8999
LID - 10.1021/acs.nanolett.2c03232 [doi]
AB  - Investigation of neural growth and connection is crucial in the field of neural 
      tissue engineering. Here, using a femtosecond laser direct writing (fs-DLW) 
      technique, we propose a directionally aligned porous microtube array as a culture 
      system for accelerating the growth of neurons and directing the connection of 
      neurites. These microtubes exhibited an unprecedented guidance effect toward the 
      outgrowth of primary embryonic rat hippocampal neurons, with a wrap resembling 
      the myelin sheaths of neurons. The speed of neurite growth inside these 
      microtubes was significantly faster than that outside these microtubes. We also 
      achieved selective/directing connection of neural networks inside the magnetic 
      microtubes via precise microtube delivery to a gap between two neural clusters. 
      This work not only proposes a powerful microtube platform for accelerated growth 
      of neurons but also offers a new idea for constructing biological neural circuits 
      by arranging the size, location, and pattern of microtubes.
FAU - Fan, Shengying
AU  - Fan S
AD  - Department of Electronic Engineering and Information Science, University of 
      Science and Technology of China, Hefei, Anhui 230026, China.
AD  - Department of Oncology, the First Affiliated Hospital of USTC, Division of Life 
      Sciences and Medicine, University of Science and Technology of China, Hefei, 
      Anhui 230001, China.
FAU - Qi, Lei
AU  - Qi L
AD  - Institute of Artificial Intelligence, Hefei Comprehensive National Science 
      Center, Hefei, Anhui 230026, China.
FAU - Li, Jiawen
AU  - Li J
AUID- ORCID: 0000-0003-3950-6212
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, CAS Key Laboratory of Mechanical Behavior and Design of Materials, 
      Department of Precision Machinery and Precision Instrumentation, University of 
      Science and Technology of China, Hefei, Anhui 230026, China.
FAU - Liu, Shunli
AU  - Liu S
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, CAS Key Laboratory of Mechanical Behavior and Design of Materials, 
      Department of Precision Machinery and Precision Instrumentation, University of 
      Science and Technology of China, Hefei, Anhui 230026, China.
FAU - Li, Rui
AU  - Li R
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, CAS Key Laboratory of Mechanical Behavior and Design of Materials, 
      Department of Precision Machinery and Precision Instrumentation, University of 
      Science and Technology of China, Hefei, Anhui 230026, China.
FAU - Zhan, Tongzhou
AU  - Zhan T
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, CAS Key Laboratory of Mechanical Behavior and Design of Materials, 
      Department of Precision Machinery and Precision Instrumentation, University of 
      Science and Technology of China, Hefei, Anhui 230026, China.
FAU - Li, Xiaowei
AU  - Li X
AD  - CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, 
      Division of Life Sciences and Medicine, University of Science and Technology of 
      China, Hefei, Anhui 230026, China.
FAU - Wu, Dong
AU  - Wu D
AUID- ORCID: 0000-0003-0623-1515
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, CAS Key Laboratory of Mechanical Behavior and Design of Materials, 
      Department of Precision Machinery and Precision Instrumentation, University of 
      Science and Technology of China, Hefei, Anhui 230026, China.
FAU - Lau, Pakming
AU  - Lau P
AD  - Institute of Artificial Intelligence, Hefei Comprehensive National Science 
      Center, Hefei, Anhui 230026, China.
AD  - CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, 
      Division of Life Sciences and Medicine, University of Science and Technology of 
      China, Hefei, Anhui 230026, China.
FAU - Qiu, Bensheng
AU  - Qiu B
AUID- ORCID: 0000-0003-2987-7378
AD  - Department of Electronic Engineering and Information Science, University of 
      Science and Technology of China, Hefei, Anhui 230026, China.
FAU - Bi, Guoqiang
AU  - Bi G
AD  - CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, 
      Division of Life Sciences and Medicine, University of Science and Technology of 
      China, Hefei, Anhui 230026, China.
FAU - Ding, Weiping
AU  - Ding W
AUID- ORCID: 0000-0002-3331-1011
AD  - Department of Oncology, the First Affiliated Hospital of USTC, Division of Life 
      Sciences and Medicine, University of Science and Technology of China, Hefei, 
      Anhui 230001, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221103
PL  - United States
TA  - Nano Lett
JT  - Nano letters
JID - 101088070
SB  - IM
MH  - Animals
MH  - Rats
MH  - *Neurites
MH  - Porosity
MH  - *Neurons/physiology
MH  - Tissue Engineering
MH  - Neurogenesis
OTO - NOTNLM
OT  - coaxial constraints
OT  - femtosecond laser writing
OT  - neurite growth
OT  - neuronal connections
OT  - porous microtubes
EDAT- 2022/11/04 06:00
MHDA- 2022/11/25 06:00
CRDT- 2022/11/03 13:32
PHST- 2022/11/04 06:00 [pubmed]
PHST- 2022/11/25 06:00 [medline]
PHST- 2022/11/03 13:32 [entrez]
AID - 10.1021/acs.nanolett.2c03232 [doi]
PST - ppublish
SO  - Nano Lett. 2022 Nov 23;22(22):8991-8999. doi: 10.1021/acs.nanolett.2c03232. Epub 
      2022 Nov 3.