PMID- 36327980
OWN - NLM
STAT- MEDLINE
DCOM- 20221124
LR  - 20230122
IS  - 1879-0445 (Electronic)
IS  - 0960-9822 (Linking)
VI  - 32
IP  - 22
DP  - 2022 Nov 21
TI  - Transient active zone remodeling in the Drosophila mushroom body supports memory.
PG  - 4900-4913.e4
LID - S0960-9822(22)01625-6 [pii]
LID - 10.1016/j.cub.2022.10.017 [doi]
AB  - Elucidating how the distinct components of synaptic plasticity dynamically 
      orchestrate the distinct stages of memory acquisition and maintenance within 
      neuronal networks remains a major challenge. Specifically, plasticity processes 
      tuning the functional and also structural state of presynaptic active zone (AZ) 
      release sites are widely observed in vertebrates and invertebrates, but their 
      behavioral relevance remains mostly unclear. We here provide evidence that a 
      transient upregulation of presynaptic AZ release site proteins supports aversive 
      olfactory mid-term memory in the Drosophila mushroom body (MB). Upon paired 
      aversive olfactory conditioning, AZ protein levels (ELKS-family 
      BRP/(m)unc13-family release factor Unc13A) increased for a few hours with 
      MB-lobe-specific dynamics. Kenyon cell (KC, intrinsic MB neurons)-specific 
      knockdown (KD) of BRP did not affect aversive olfactory short-term memory (STM) 
      but strongly suppressed aversive mid-term memory (MTM). Different proteins 
      crucial for the transport of AZ biosynthetic precursors (transport adaptor 
      Aplip1/Jip-1; kinesin motor IMAC/Unc104; small GTPase Arl8) were also 
      specifically required for the formation of aversive olfactory MTM. Consistent 
      with the merely transitory increase of AZ proteins, BRP KD did not interfere with 
      the formation of aversive olfactory long-term memory (LTM; i.e., 1 day). Our data 
      suggest that the remodeling of presynaptic AZ refines the MB circuitry after 
      paired aversive conditioning, over a time window of a few hours, to display 
      aversive olfactory memories.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Turrel, Oriane
AU  - Turrel O
AD  - Institute for Biology/Genetics, Freie Universitat Berlin, Takustrasse 6, 14195 
      Berlin, Germany.
FAU - Ramesh, Niraja
AU  - Ramesh N
AD  - Institute for Biology/Genetics, Freie Universitat Berlin, Takustrasse 6, 14195 
      Berlin, Germany.
FAU - Escher, Marc J F
AU  - Escher MJF
AD  - Institute for Biology/Genetics, Freie Universitat Berlin, Takustrasse 6, 14195 
      Berlin, Germany.
FAU - Pooryasin, Atefeh
AU  - Pooryasin A
AD  - Institute for Biology/Genetics, Freie Universitat Berlin, Takustrasse 6, 14195 
      Berlin, Germany.
FAU - Sigrist, Stephan J
AU  - Sigrist SJ
AD  - Institute for Biology/Genetics, Freie Universitat Berlin, Takustrasse 6, 14195 
      Berlin, Germany; NeuroCure Cluster of Excellence, Charite Universitatsmedizin, 
      Chariteplatz 1, 10117 Berlin, Germany. Electronic address: 
      stephan.sigrist@fu-berlin.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221102
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Drosophila Proteins)
SB  - IM
MH  - Animals
MH  - *Mushroom Bodies/physiology
MH  - Drosophila/physiology
MH  - *Drosophila Proteins/metabolism
MH  - Avoidance Learning/physiology
MH  - Memory, Short-Term/physiology
OTO - NOTNLM
OT  - Drosophila
OT  - active zone
OT  - activity-dependent remodeling
OT  - memory
OT  - presynaptic plasticity
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2022/11/04 06:00
MHDA- 2022/11/25 06:00
CRDT- 2022/11/03 19:43
PHST- 2021/12/16 00:00 [received]
PHST- 2022/08/15 00:00 [revised]
PHST- 2022/10/06 00:00 [accepted]
PHST- 2022/11/04 06:00 [pubmed]
PHST- 2022/11/25 06:00 [medline]
PHST- 2022/11/03 19:43 [entrez]
AID - S0960-9822(22)01625-6 [pii]
AID - 10.1016/j.cub.2022.10.017 [doi]
PST - ppublish
SO  - Curr Biol. 2022 Nov 21;32(22):4900-4913.e4. doi: 10.1016/j.cub.2022.10.017. Epub 
      2022 Nov 2.