PMID- 36328251
OWN - NLM
STAT- MEDLINE
DCOM- 20230113
LR  - 20230113
IS  - 1743-1328 (Electronic)
IS  - 0161-6412 (Linking)
VI  - 45
IP  - 1
DP  - 2023 Jan
TI  - CircNUFIP2 overexpression induces GDF11 to ameliorate oxygen-glucose 
      deprivation-induced hippocampal neuron cell apoptosis and oxidative stress after 
      cerebral ischemia.
PG  - 70-80
LID - 10.1080/01616412.2022.2123172 [doi]
AB  - Background Previous data have indicated the regulation of circular RNA (circRNA) 
      toward cerebral ischemia. This study aims to reveal the effects of circNUFIP2 on 
      cerebral ischemia and the underlying mechanism. Methods Oxygen-glucose 
      deprivation (OGD) hippocampal neuron (HT22) cell model and middle cerebral artery 
      occlusion (MCAO) mouse model were used for this study. The expression of circRNA 
      nuclear FMR1 interacting protein 2 (circNUFIP2), microRNA-1224-5p (miR-1224-5p) 
      and growth differentiation factor 11 (GDF11) was detected by quantitative 
      real-time polymerase-chain reaction. Protein expression was checked by Western 
      blotting. The binding relationships among circNUFIP2, miR-1224-5p and GDF11 were 
      identified by dual-luciferase reporter assay, RNA pull-down assay, and RNA 
      immunoprecipitation assay. Cell proliferation and apoptosis were investigated by 
      5-Ethynyl-29-deoxyuridine and flow cytometry analysis, respectively. Results 
      CircNUFIP2 and GDF11 expression were decreased, but miR-1224-5p was increased in 
      OGD-treated HT22 cells when compared with their expression in control groups. OGD 
      treatment inhibited HT22 cell proliferation but induced cell apoptosis and 
      oxidative stress; however, these effects were attenuated after circNUFIP2 
      overexpression. Also, circNUFIP2 upregulation assuaged the cerebral infarction of 
      MCAO mice. Besides, circNUFIP2 bound to miR-1224-5p and mediated OGD-induced HT22 
      cell damage through miR-1224-5p. Meanwhile, knockdown of GDF11, a target gene of 
      miR-1224-5p, relieved miR-1224-5p depletion-caused effects in OGD-treated HT22 
      cells. Furthermore, circNUFIP2 regulated GDF11 expression by interacting with 
      miR-1224-5p. Conclusion CircNUFIP2 overexpression protected neuron cells against 
      cerebral ischemia-induced damage, at least in part, by the miR-1224-5p/GDF11 
      pathway, providing a possible target for the therapy of cerebral ischemic stroke.
FAU - Mei, Zhujun
AU  - Mei Z
AD  - Department of Neurology, Jiangxi Provincial People's Hospital, the First 
      Affiliated Hospital of Nanchang Medical College, Nanchang City, Jiangxi Province, 
      China.
FAU - Huang, LinLing
AU  - Huang L
AD  - Department of Gynecology, Jiangxi Provincial Maternal and Child Health Hospital, 
      Nanchang City, Jiangxi Province, China.
FAU - Rao, Wei
AU  - Rao W
AD  - Department of Neurology, Jiangxi Provincial People's Hospital, the First 
      Affiliated Hospital of Nanchang Medical College, Nanchang City, Jiangxi Province, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20221103
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - S88TT14065 (Oxygen)
RN  - IY9XDZ35W2 (Glucose)
RN  - 0 (RNA, Circular)
RN  - 0 (MicroRNAs)
RN  - 0 (Gdf11 protein, mouse)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Growth Differentiation Factors)
RN  - 0 (Fmr1 protein, mouse)
RN  - 0 (MIRN1224 microRNA, mouse)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Oxygen
MH  - Glucose/metabolism
MH  - RNA, Circular/metabolism/pharmacology
MH  - *MicroRNAs/genetics/metabolism
MH  - *Brain Ischemia/metabolism
MH  - *Stroke/metabolism
MH  - Infarction, Middle Cerebral Artery/metabolism
MH  - Oxidative Stress
MH  - Apoptosis
MH  - Hippocampus/metabolism
MH  - Neurons/metabolism
MH  - *Reperfusion Injury/metabolism
MH  - Bone Morphogenetic Proteins/metabolism/pharmacology
MH  - Growth Differentiation Factors/genetics/metabolism/pharmacology
OTO - NOTNLM
OT  - GDF11
OT  - MCAO
OT  - circNUFIP2
OT  - ischemic stroke
OT  - miR-1224-5p
EDAT- 2022/11/04 06:00
MHDA- 2023/01/14 06:00
CRDT- 2022/11/03 20:33
PHST- 2022/11/04 06:00 [pubmed]
PHST- 2023/01/14 06:00 [medline]
PHST- 2022/11/03 20:33 [entrez]
AID - 10.1080/01616412.2022.2123172 [doi]
PST - ppublish
SO  - Neurol Res. 2023 Jan;45(1):70-80. doi: 10.1080/01616412.2022.2123172. Epub 2022 
      Nov 3.