PMID- 36331009
OWN - NLM
STAT- MEDLINE
DCOM- 20230222
LR  - 20230222
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 37
IP  - 2
DP  - 2023 Feb
TI  - Salidroside alleviates ulcerative colitis via inhibiting macrophage pyroptosis 
      and repairing the dysbacteriosis-associated Th17/Treg imbalance.
PG  - 367-382
LID - 10.1002/ptr.7636 [doi]
AB  - Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by 
      flora disequilibrium and mucosal immunity disorder. Here, we report that 
      salidroside effectively restricts experimental colitis from two aspects of 
      intestinal macrophage pyroptosis and dysbacteriosis-derived colonic Th17/Treg 
      imbalance. In innate immunity, the upregulated TREM1 and pyroptosis-related 
      proteins in inflamed colons were inhibited by salidroside administration and 
      further experiments in vitro showed that salidroside suppressed LPS/ATP-induced 
      bone marrow-derived macrophages (BMDMs) pyroptosis evident by the decline of LDH 
      and IL-1beta release as well as the protein level of NLRP3, caspase-1, and GSDMD 
      p30. Moreover, the TREM1 inhibitor weakened the effect of salidroside on BMDMs 
      pyroptosis, whereas salidroside still could downregulate TREM1 when NLRP3 was 
      inhibited. In adaptive immunity, salidroside improved the gut microflora 
      diversity and Th17/Treg ratio in DSS-induced mice, especially promoting the 
      abundance of Firmicutes. Clearance of the gut flora blocked the benefit of 
      salidroside on colonic inflammation and Th17/Treg adaptive immunity, but 
      transplanting salidroside-treated foecal bacterium into flora-depleted wild mice 
      reproduced the resistance of salidroside to gut inflammation. Taken together, our 
      data demonstrated that salidroside protected experimental colitis via skewing 
      macrophage pyroptosis and Th17/Treg balance, indicating its potential effect on 
      UC and other immune disorders.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Liu, Xiaoman
AU  - Liu X
AUID- ORCID: 0000-0002-2371-4570
AD  - Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University 
      School of Medicine, Shanghai, China.
AD  - Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, 
      China.
FAU - Zhou, Mingxia
AU  - Zhou M
AD  - Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, 
      China.
FAU - Dai, Zhenzhen
AU  - Dai Z
AD  - Shanghai Institute for Pediatric Research, Shanghai, China.
FAU - Luo, Shangjian
AU  - Luo S
AD  - Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University 
      School of Medicine, Shanghai, China.
AD  - Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, 
      China.
FAU - Shi, Yingying
AU  - Shi Y
AD  - Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University 
      School of Medicine, Shanghai, China.
AD  - Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, 
      China.
FAU - He, Zhenjuan
AU  - He Z
AD  - Department of Neonatology, Xinhua Hospital, Shanghai Jiaotong University School 
      of Medicine, Shanghai, China.
FAU - Chen, Yingwei
AU  - Chen Y
AD  - Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University 
      School of Medicine, Shanghai, China.
LA  - eng
GR  - 8197030824/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20221104
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - M983H6N1S9 (rhodioloside)
RN  - 0 (Triggering Receptor Expressed on Myeloid Cells-1)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Colitis, Ulcerative/chemically induced/drug therapy
MH  - Triggering Receptor Expressed on Myeloid Cells-1/metabolism
MH  - Pyroptosis
MH  - T-Lymphocytes, Regulatory/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Dysbiosis
MH  - *Colitis/chemically induced
MH  - Macrophages/metabolism
MH  - Inflammation/metabolism
MH  - Dextran Sulfate/adverse effects
MH  - Mice, Inbred C57BL
OTO - NOTNLM
OT  - IBD
OT  - Salidroside
OT  - TREM1 signalling
OT  - gut microbiota
OT  - pyroptosis
EDAT- 2022/11/05 06:00
MHDA- 2023/02/22 06:00
CRDT- 2022/11/04 06:32
PHST- 2022/09/03 00:00 [revised]
PHST- 2022/06/16 00:00 [received]
PHST- 2022/09/14 00:00 [accepted]
PHST- 2022/11/05 06:00 [pubmed]
PHST- 2023/02/22 06:00 [medline]
PHST- 2022/11/04 06:32 [entrez]
AID - 10.1002/ptr.7636 [doi]
PST - ppublish
SO  - Phytother Res. 2023 Feb;37(2):367-382. doi: 10.1002/ptr.7636. Epub 2022 Nov 4.