PMID- 36332472
OWN - NLM
STAT- MEDLINE
DCOM- 20221116
LR  - 20221208
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 636
IP  - Pt 1
DP  - 2022 Dec 25
TI  - Stomatin-like protein-2 attenuates macrophage pyroptosis and H9c2 cells apoptosis 
      by protecting mitochondrial function.
PG  - 112-120
LID - S0006-291X(22)01441-3 [pii]
LID - 10.1016/j.bbrc.2022.10.047 [doi]
AB  - Myocytes undergoing hypoxia condition can recruit macrophages and cause 
      pro-inflammation initiation around the injury area. Mitochondrial dysfunction is 
      related to macrophage pyroptosis. Stomatin-like protein-2 (SLP-2) can regulate 
      mitochondrial biogenesis and function. Whether SLP-2 could affect macrophage 
      pyroptosis remains unclear. In this study, bone marrow derived macrophages 
      (BMDMs) were extracted from WT and SLP-2 knocked out mice, then stimulated by 
      LPS/Nigericin. Western blot showed that SLP-2(-/-) promoted the expression of 
      NLRP3, GSDMD-N, caspase-11 in macrophages, which means the deficiency of SLP-2 
      augments macrophage pyroptosis. Higher fluorescence intensity of dihydroethidium 
      and TUNEL represented the increased ROS releasing and macrophage programmed death 
      in SLP-2 deficiency groups. The immunofluorescence intensity of MtioTracker Red 
      decreased and that of mitochondrial ROS (mtROS) increased in SLP-2 deletion 
      groups with LPS/Nigericin stimulation, representing the increased mitochondrial 
      damage. The expression level of HIF-1alpha increased in SLP-2 deletion macrophages 
      with LPS and Nigericin stimulation. The level of Parkin and the ratio of LC3II/I 
      decreased in SLP-2 deficiency macrophages after stimulated by LPS/Nigericin, 
      compared with untreated macrophages. H9c2 cells were cultured in hypoxia 
      condition before being cocultured with macrophage supernatant. The cocultured 
      H9c2 cells were injured due to the serious pyroptosis of SLP-2 deficiency 
      macrophages. According to these results, we suggest that SLP-2 can reduce 
      macrophage pyroptosis and relieve hypoxia H9c2 cells injury through protecting 
      mitochondrial function.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Fan, Rui
AU  - Fan R
AD  - School of Medicine, Southeast University, Nanjing, Jiangsu, 210009, China; 
      Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China.
FAU - Jiang, Hongwei
AU  - Jiang H
AD  - Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China.
FAU - Hu, Yuntao
AU  - Hu Y
AD  - Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China.
FAU - Xu, Yueyue
AU  - Xu Y
AD  - Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China.
FAU - Zhou, Yifei
AU  - Zhou Y
AD  - Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China.
FAU - Chen, Ganyi
AU  - Chen G
AD  - Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China.
FAU - Liu, Yafeng
AU  - Liu Y
AD  - Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China.
FAU - Yao, Yiwei
AU  - Yao Y
AD  - Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China.
FAU - Qin, Wei
AU  - Qin W
AD  - Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China.
FAU - Chen, Wen
AU  - Chen W
AD  - Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China. Electronic address: 
      cxacf@163.com.
FAU - Huang, Fuhua
AU  - Huang F
AD  - Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China. Electronic address: 
      huangfuhua@sina.com.
FAU - Chen, Xin
AU  - Chen X
AD  - School of Medicine, Southeast University, Nanjing, Jiangsu, 210009, China; 
      Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, 
      Nanjing Medical University, Nanjing, Jiangsu, 210006, China. Electronic address: 
      stevecx@njmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221020
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Lipopolysaccharides)
RN  - RRU6GY95IS (Nigericin)
RN  - 0 (Inflammasomes)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Pyroptosis
MH  - *NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Lipopolysaccharides/metabolism
MH  - Nigericin
MH  - Macrophages/metabolism
MH  - Mitochondria/metabolism
MH  - Hypoxia/metabolism
MH  - Inflammasomes/metabolism
OTO - NOTNLM
OT  - Macrophage pyroptosis
OT  - Mitochondrial dysfunction
OT  - SLP-2
OT  - STOML2
OT  - Stomatin-like protein-2
OT  - mtROS
COIS- Declaration of competing interest There is no conflict of interest regarding the 
      publication of this paper.
EDAT- 2022/11/05 06:00
MHDA- 2022/11/18 06:00
CRDT- 2022/11/04 19:23
PHST- 2022/10/01 00:00 [received]
PHST- 2022/10/03 00:00 [revised]
PHST- 2022/10/12 00:00 [accepted]
PHST- 2022/11/05 06:00 [pubmed]
PHST- 2022/11/18 06:00 [medline]
PHST- 2022/11/04 19:23 [entrez]
AID - S0006-291X(22)01441-3 [pii]
AID - 10.1016/j.bbrc.2022.10.047 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2022 Dec 25;636(Pt 1):112-120. doi: 
      10.1016/j.bbrc.2022.10.047. Epub 2022 Oct 20.