PMID- 36333756
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221108
IS  - 2055-5660 (Print)
IS  - 2055-5660 (Electronic)
IS  - 2055-5660 (Linking)
VI  - 8
IP  - 1
DP  - 2022 Nov 4
TI  - In the presence of non-neutralising maternally derived antibodies, intradermal 
      and intramuscular vaccination with a modified live vaccine against porcine 
      reproductive and respiratory syndrome virus 1 (PRRSV-1) induce similar levels of 
      neutralising antibodies or interferon-gamma secreting cells.
PG  - 47
LID - 10.1186/s40813-022-00289-4 [doi]
LID - 47
AB  - The purpose of this study was to compare the immune response generated by the 
      intramuscular and the intradermal vaccination route against the porcine 
      reproductive and respiratory syndrome virus (PRRSV). Piglets from a seronegative 
      and a seropositive farm were selected (n = 28 piglets per farm), and each group 
      was divided into two groups and vaccinated after weaning with modified live 
      vaccine Unistrain(R) PRRS (Laboratorios Hipra Amer, Spain) by the intramuscular 
      (IM) or the intradermic (ID) route. For the following 6 weeks, animals were 
      weekly bled to assess the humoral response by PRRSV-specific antibody ELISA and 
      viral neutralisation test. At 0-, 3-, 4- and 6 weeks post-vaccination, peripheral 
      mononuclear blood cells (PBMC) from eight animals per group were recovered to 
      analyse cellular response by IFN-gamma ELISPOT and lymphoproliferation. Serum IL-12 
      was also quantified by ELISA. Seroconversion was first detected 14 days 
      post-vaccination (dpv) for both IM and ID routes, and peaked at 35 dpv (both IM 
      groups and ID seropositive) or 42 dpv (ID seronegative). At 3 weeks after 
      vaccination, 6/27 (22.22%) animals from negative origin had not seroconverted, 
      and neutralising titres were significantly lower at 35 dpv compared to the 
      seropositive origin (mean log(2) titres of 1.36 and 4.25 respectively) Also, it 
      was 10 times more probable for them to have high levels of IL-12 a week after 
      vaccination than for animals of seropositive origin. Cellular immune response 
      analysed by lymphoproliferation and IFN-gamma ELISPOT was already present at 21 dpv 
      and until 42 dpv, with no significant differences between groups except for a 
      higher lymphoproliferation at 35 dpv in the IM seropositive group 
      (Kruskal-Wallis, p < 0.05). These results indicate that the intradermal route 
      induces an immune response equivalent to the classical intramuscular route even 
      in presence of non-neutralising maternal immunity, which in this study has proven 
      to facilitate seroconversion after vaccination with an heterologous strain.
CI  - (c) 2022. The Author(s).
FAU - Aguirre, Laia
AU  - Aguirre L
AD  - Departament de Sanitat i Anatomia Animals, Universitat Autonoma de Barcelona 
      (UAB), 08193, Cerdanyola del Valles, Spain. laia.aguirre@uab.cat.
FAU - Li, Yanli
AU  - Li Y
AD  - Departament de Sanitat i Anatomia Animals, Universitat Autonoma de Barcelona 
      (UAB), 08193, Cerdanyola del Valles, Spain.
FAU - Baratelli, Massimiliano
AU  - Baratelli M
AD  - HIPRA, Amer (Girona), Girona, Spain.
FAU - Martin-Valls, Gerard
AU  - Martin-Valls G
AD  - Departament de Sanitat i Anatomia Animals, Universitat Autonoma de Barcelona 
      (UAB), 08193, Cerdanyola del Valles, Spain.
FAU - Cortey, Marti
AU  - Cortey M
AD  - Departament de Sanitat i Anatomia Animals, Universitat Autonoma de Barcelona 
      (UAB), 08193, Cerdanyola del Valles, Spain.
FAU - Miranda, Joel
AU  - Miranda J
AD  - HIPRA, Amer (Girona), Girona, Spain.
FAU - Martin, Marga
AU  - Martin M
AD  - Departament de Sanitat i Anatomia Animals, Universitat Autonoma de Barcelona 
      (UAB), 08193, Cerdanyola del Valles, Spain.
FAU - Mateu, Enric
AU  - Mateu E
AD  - Departament de Sanitat i Anatomia Animals, Universitat Autonoma de Barcelona 
      (UAB), 08193, Cerdanyola del Valles, Spain.
LA  - eng
PT  - Journal Article
DEP - 20221104
PL  - England
TA  - Porcine Health Manag
JT  - Porcine health management
JID - 101684126
PMC - PMC9636649
OTO - NOTNLM
OT  - Intradermal
OT  - Intramuscular
OT  - Maternally derived antibodies
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Vaccine
COIS- Massimiliano Baratelli and Joel Miranda are employees of Laboratorios Hipra.
EDAT- 2022/11/06 06:00
MHDA- 2022/11/06 06:01
PMCR- 2022/11/04
CRDT- 2022/11/05 00:57
PHST- 2022/07/14 00:00 [received]
PHST- 2022/10/11 00:00 [accepted]
PHST- 2022/11/05 00:57 [entrez]
PHST- 2022/11/06 06:00 [pubmed]
PHST- 2022/11/06 06:01 [medline]
PHST- 2022/11/04 00:00 [pmc-release]
AID - 10.1186/s40813-022-00289-4 [pii]
AID - 289 [pii]
AID - 10.1186/s40813-022-00289-4 [doi]
PST - epublish
SO  - Porcine Health Manag. 2022 Nov 4;8(1):47. doi: 10.1186/s40813-022-00289-4.