PMID- 36338035
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221108
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar 
      macrophages.
PG  - 1022481
LID - 10.3389/fmicb.2022.1022481 [doi]
LID - 1022481
AB  - The widespread and endemic circulation of porcine reproductive and respiratory 
      syndrome virus (PRRSV) cause persistent financial losses to the swine industry 
      worldwide. In 2017, NADC34-like PRRSV-2 emerged in northeastern China and spread 
      rapidly. The dynamics analysis of immune perturbations associated with novel 
      PRRSV lineage is still incomplete. This study performed a time-course 
      transcriptome sequencing of NADC34-like PRRSV strain YC-2020-infected porcine 
      alveolar macrophages (PAMs) and compared them with JXA1-infected PAMs. The 
      results illustrated dramatic changes in the host's differentially expressed genes 
      (DEGs) presented at different timepoints after PRRSV infection, and the 
      expression profile of YC-2020 group is distinct from that of JXA1 group. 
      Functional enrichment analysis showed that the expression of many inflammatory 
      cytokines was up-regulated following YC-2020 infection but at a significantly 
      lower magnitude than JXA1 group, in line with the trends for most 
      interferon-stimulated genes (ISGs) and their regulators. Meanwhile, numerous 
      components of histocompatibility complex (MHC) class II and phagosome presented a 
      stronger transcription suppression after the YC-2020 infection. All results imply 
      that YC-2020 may induce milder inflammatory responses, weaker antiviral 
      processes, and more severe disturbance of antigen processing and presentation 
      compared with HP-PRRSV. Additionally, LAPTM4A, GLMP, and LITAF, which were 
      selected from weighted gene co-expression network analysis (WGCNA), could 
      significantly inhibit PRRSV proliferation. This study provides fundamental data 
      for understanding the biological characteristics of NADC34-like PRRSV and new 
      insights into PRRSV evolution and prevention.
CI  - Copyright (c) 2022 Wang, Ma, Zhang, Zhao, Hu, Luo, Zeshan, Yang, Qiu, Wang, Liu, 
      Zhou and Wang.
FAU - Wang, Peixin
AU  - Wang P
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Ma, Xin
AU  - Ma X
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Zhang, Riteng
AU  - Zhang R
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Zhao, Yongxin
AU  - Zhao Y
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Hu, Ruochen
AU  - Hu R
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Luo, Chen
AU  - Luo C
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Zeshan, Basit
AU  - Zeshan B
AD  - Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, Sandakan, Sabah, 
      Malaysia.
FAU - Yang, Zengqi
AU  - Yang Z
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Qiu, Li
AU  - Qiu L
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Wang, Juan
AU  - Wang J
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Liu, Haijin
AU  - Liu H
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Zhou, Yefei
AU  - Zhou Y
AD  - Department of Life Science, Nanjing Xiaozhuang University, Nanjing, Jiangsu, 
      China.
FAU - Wang, Xinglong
AU  - Wang X
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20221019
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9629508
OTO - NOTNLM
OT  - NADC34-like
OT  - PRRSV
OT  - WGCNA
OT  - comparative transcriptome
OT  - time-course transcriptome
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/11/08 06:00
MHDA- 2022/11/08 06:01
PMCR- 2022/10/19
CRDT- 2022/11/07 04:21
PHST- 2022/08/21 00:00 [received]
PHST- 2022/09/15 00:00 [accepted]
PHST- 2022/11/07 04:21 [entrez]
PHST- 2022/11/08 06:00 [pubmed]
PHST- 2022/11/08 06:01 [medline]
PHST- 2022/10/19 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.1022481 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Oct 19;13:1022481. doi: 10.3389/fmicb.2022.1022481. 
      eCollection 2022.