PMID- 36338387
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221108
IS  - 2666-9102 (Electronic)
IS  - 2666-9102 (Linking)
VI  - 2
IP  - 5
DP  - 2021 Sep
TI  - Characterization of milk and soy phospholipid liposomes and inflammation in 
      3T3-L1 adipocytes.
PG  - 238-242
LID - 10.3168/jdsc.2020-0054 [doi]
AB  - Milk phospholipids (PL) are valuable dairy components that appear to impart human 
      health benefits, including improved cognitive function in infants and adults. The 
      commercial food industry uses primarily plant-based sources of PL, such as soy 
      lecithin. However, it remains unclear whether different compositions of PL from 
      different dietary sources, such as milk, convey the same benefits. We 
      hypothesized that PL derived from bovine milk or soy have differing physiological 
      effects in terms of inflammation due to their differences in composition. The 
      objectives of this study were to characterize milk and soy liposomes by their 
      physicochemical properties and composition and to evaluate their effects in vitro 
      by means of inflammatory gene expression analyses. Milk and soy phospholipid 
      large unilamellar vesicles (MPL-LUV and SPL-LUV, respectively) prepared using 
      thin-film hydration coupled with extrusion were similar in terms of structure, 
      size, and stability; however, they differed significantly in composition. The 
      3T3-L1 adipocytes were selected for this work because adipocytes are the main 
      site of uptake, synthesis, modification, and breakdown of lipids and are 
      important inflammatory mediators in mammalian systems. In this work, these cells 
      exposed to both liposome varieties showed high biocompatibility and low 
      cytotoxicity up to concentrations of 0.5 mg/mL as measured by colorimetric MTT 
      and lactate dehydrogenase assays. Furthermore, SPL-LUV showed trends toward 
      stimulating inflammation compared with MPL-LUV as measured by expression of 2 
      proinflammatory cytokines, monocyte chemoattractant protein-1 (MCP-1) and IL-6. 
      Expression of MCP-1 significantly increased 1.82-fold relative to the control 
      upon SPL-LUV treatment, with similar trends for IL-6 (increased 1.59-fold). The 
      MPL-LUV showed relatively no change in cytokine expression. The results obtained 
      in this work suggest that the methodology used to prepare LUV and the composition 
      and proportion of milk PL are important in measuring cell physiology changes and 
      inflammatory status in mammalian cells.
CI  - (c) 2021.
FAU - Kosmerl, Erica
AU  - Kosmerl E
AD  - Department of Food Science and Technology, The Ohio State University, Columbus 
      43210.
FAU - Garcia-Cano, Israel
AU  - Garcia-Cano I
AD  - Department of Food Science and Technology, The Ohio State University, Columbus 
      43210.
FAU - Rocha-Mendoza, Diana
AU  - Rocha-Mendoza D
AD  - Department of Food Science and Technology, The Ohio State University, Columbus 
      43210.
FAU - Jimenez-Flores, Rafael
AU  - Jimenez-Flores R
AD  - Department of Food Science and Technology, The Ohio State University, Columbus 
      43210.
LA  - eng
PT  - Journal Article
DEP - 20210619
PL  - United States
TA  - JDS Commun
JT  - JDS communications
JID - 9918300983806676
PMC - PMC9623641
EDAT- 2021/06/19 00:00
MHDA- 2021/06/19 00:01
PMCR- 2021/06/19
CRDT- 2022/11/07 04:27
PHST- 2020/11/13 00:00 [received]
PHST- 2021/05/09 00:00 [accepted]
PHST- 2022/11/07 04:27 [entrez]
PHST- 2021/06/19 00:00 [pubmed]
PHST- 2021/06/19 00:01 [medline]
PHST- 2021/06/19 00:00 [pmc-release]
AID - S2666-9102(21)00075-2 [pii]
AID - 10.3168/jdsc.2020-0054 [doi]
PST - epublish
SO  - JDS Commun. 2021 Jun 19;2(5):238-242. doi: 10.3168/jdsc.2020-0054. eCollection 
      2021 Sep.