PMID- 36339495
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221108
IS  - 2666-9102 (Electronic)
IS  - 2666-9102 (Linking)
VI  - 2
IP  - 3
DP  - 2021 May
TI  - Sodium salicylate reduced mRNA abundance of hypoxia-associated genes in MAC-T 
      cells.
PG  - 159-164
LID - 10.3168/jdsc.2020-0037 [doi]
AB  - Hypoxia is an oxygen deficiency commonly found in growing tissues and is 
      speculated to occur in the rapidly developing mammary gland in peripartum dairy 
      cattle. Low oxygen concentrations can activate hypoxia-inducible factor-1 
      (HIF-1), which increases transcription of genes involved in angiogenesis (VEGFA) 
      and glucose transport (GLUT1), among other processes. The mRNA stability of these 
      genes is positively regulated by heterogeneous nuclear ribonucleoprotein D 
      (HNRNPD; also known as AUF1). In our previous research, postpartum administration 
      of sodium salicylate (SS) increased whole-lactation milk yield in multiparous 
      cows but tended to reduce milk yield in primiparous cows. Because rapid mammary 
      tissue development likely occurs in cows approaching first lactation, we 
      hypothesized that SS inhibited the activation of HIF-1alpha and decreased 
      transcription of downstream targets. MAC-T cells were treated with SS (100 muM) or 
      control medium before incubation under either hypoxic (1% O(2)) or normoxic 
      conditions for 12 h. Additionally, cells were transfected with either HIF1A small 
      interfering RNA (siRNA) or a scrambled siRNA negative control 48 h before hypoxia 
      treatments. HIF1A, GLUT1, VEGFA, and HNRNPD were quantified relative to the 
      internal control gene NENF. Transcript abundance was assessed using a linear 
      mixed model with the fixed effects of SS, hypoxia, siRNA, and all 2- and 3-way 
      interaction terms and the random effect of plate nested within hypoxia. Treatment 
      with SS interacted with hypoxia for GLUT1, as SS reduced GLUT1 when MAC-T cells 
      were cultured in normoxic conditions; however, no effect of SS was found in 
      hypoxia-treated cells. Regardless of oxygen status, SS reduced HNRNPD and tended 
      to decrease VEGFA mRNA relative to untreated cells. Hypoxia increased GLUT1, yet 
      no effect was observed on VEGFA or HNRNPD. Small interfering RNA knocked down 
      HIF1A, but no effect was found on GLUT1, VEGFA, or HNRNPD. In conclusion, SS 
      reduced transcript abundance of genes involved with mammary gland development but 
      generally did not interact with oxygen status.
CI  - (c) 2021.
FAU - Ylioja, C M
AU  - Ylioja CM
AD  - Department of Animal Sciences and Industry, Kansas State University, Manhattan 
      66506.
FAU - Swartz, T H
AU  - Swartz TH
AD  - Department of Animal Sciences and Industry, Kansas State University, Manhattan 
      66506.
AD  - Department of Animal Science, Michigan State University, East Lansing 48824.
FAU - Mamedova, L K
AU  - Mamedova LK
AD  - Department of Animal Sciences and Industry, Kansas State University, Manhattan 
      66506.
AD  - Department of Animal Science, Michigan State University, East Lansing 48824.
FAU - Bradford, B J
AU  - Bradford BJ
AD  - Department of Animal Sciences and Industry, Kansas State University, Manhattan 
      66506.
AD  - Department of Animal Science, Michigan State University, East Lansing 48824.
LA  - eng
PT  - Journal Article
DEP - 20210326
PL  - United States
TA  - JDS Commun
JT  - JDS communications
JID - 9918300983806676
PMC - PMC9623739
EDAT- 2021/03/26 00:00
MHDA- 2021/03/26 00:01
PMCR- 2021/03/26
CRDT- 2022/11/07 04:46
PHST- 2020/10/05 00:00 [received]
PHST- 2021/02/07 00:00 [accepted]
PHST- 2022/11/07 04:46 [entrez]
PHST- 2021/03/26 00:00 [pubmed]
PHST- 2021/03/26 00:01 [medline]
PHST- 2021/03/26 00:00 [pmc-release]
AID - S2666-9102(21)00041-7 [pii]
AID - 10.3168/jdsc.2020-0037 [doi]
PST - epublish
SO  - JDS Commun. 2021 Mar 26;2(3):159-164. doi: 10.3168/jdsc.2020-0037. eCollection 
      2021 May.