PMID- 36339649
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221108
IS  - 2768-2811 (Electronic)
IS  - 2768-2811 (Linking)
VI  - 1
IP  - 4
DP  - 2022 Apr
TI  - Single vesicle analysis of aqueous humor in pediatric ocular diseases reveals eye 
      specific CD63-dominant subpopulations.
LID - e36 [pii]
LID - 10.1002/jex2.36 [doi]
AB  - Aqueous humor (AH), the clear fluid in front of the eye, maintains the pressure 
      and vitality of ocular tissues. This fluid is accessible via the clear cornea 
      which enables use of AH as a liquid biopsy source of biomarkers for intraocular 
      disease. Extracellular vesicles are detectable in the AH and small extracellular 
      vesicles (sEVs) are present in the AH from adults. However, EVs in AH from 
      pediatric eyes in vivo have never previously been explored. We know very little 
      about the heterogeneity of AH EV populations in ocular disease. Twenty-seven 
      processing-free AH samples from 19 patients across four different pediatric 
      ocular diseases were subjected to Nanoparticle Tracking Analysis (NTA) and Single 
      Particle-Interferometric Reflectance Imaging Sensor (SP-IRIS) analysis. NTA 
      demonstrated the concentration of AH EV/EPs is 3.11 x 10(9)-1.38 x 10(10) 
      particles/ml; the majority sized 76.8-103 nm. SP-IRIS revealed distinct patterns 
      of tetraspanin expression of AH sEVs. An enriched mono-CD63+ sEV subpopulation 
      identified in AH indicates this is a potential AH-specific biomarker. In the 
      setting of retinoblastoma there was a more heterogeneous population of sEVs which 
      normalized with treatment. This suggests a potential clinical application of 
      direct measurement of sEV subpopulations in AH samples to monitor successful 
      tumor response to therapy.
FAU - Peng, Chen-Ching
AU  - Peng CC
AUID- ORCID: 0000-0002-2703-1275
AD  - The Vision Center at Children's Hospital Los Angeles, Los Angeles, California, 
      USA.
AD  - USC Roski Eye Institute, Keck School of Medicine of the University of Southern 
      California, Los Angeles, California, USA.
FAU - Im, Deborah
AU  - Im D
AUID- ORCID: 0000-0002-7126-4615
AD  - The Vision Center at Children's Hospital Los Angeles, Los Angeles, California, 
      USA.
AD  - USC Roski Eye Institute, Keck School of Medicine of the University of Southern 
      California, Los Angeles, California, USA.
FAU - Sirivolu, Shreya
AU  - Sirivolu S
AD  - The Vision Center at Children's Hospital Los Angeles, Los Angeles, California, 
      USA.
AD  - USC Roski Eye Institute, Keck School of Medicine of the University of Southern 
      California, Los Angeles, California, USA.
FAU - Reiser, Bibiana
AU  - Reiser B
AD  - The Vision Center at Children's Hospital Los Angeles, Los Angeles, California, 
      USA.
AD  - USC Roski Eye Institute, Keck School of Medicine of the University of Southern 
      California, Los Angeles, California, USA.
AD  - The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, 
      California, USA.
FAU - Nagiel, Aaron
AU  - Nagiel A
AD  - The Vision Center at Children's Hospital Los Angeles, Los Angeles, California, 
      USA.
AD  - USC Roski Eye Institute, Keck School of Medicine of the University of Southern 
      California, Los Angeles, California, USA.
AD  - The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, 
      California, USA.
FAU - Neviani, Paolo
AU  - Neviani P
AD  - The Extracellular Vesicle Core at Children's Hospital Los Angeles, Los Angeles, 
      California, USA.
FAU - Xu, Liya
AU  - Xu L
AUID- ORCID: 0000-0002-5501-2039
AD  - The Vision Center at Children's Hospital Los Angeles, Los Angeles, California, 
      USA.
AD  - USC Roski Eye Institute, Keck School of Medicine of the University of Southern 
      California, Los Angeles, California, USA.
FAU - Berry, Jesse L
AU  - Berry JL
AUID- ORCID: 0000-0002-8907-9100
AD  - The Vision Center at Children's Hospital Los Angeles, Los Angeles, California, 
      USA.
AD  - USC Roski Eye Institute, Keck School of Medicine of the University of Southern 
      California, Los Angeles, California, USA.
AD  - The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, 
      California, USA.
AD  - Norris Comprehensive Cancer Center, Keck School of Medicine, University of 
      Southern California, Los Angeles, California, USA.
LA  - eng
GR  - K08 CA232344/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20220420
PL  - United States
TA  - J Extracell Biol
JT  - Journal of extracellular biology
JID - 9918382980506676
PMC - PMC9632627
MID - NIHMS1815535
OTO - NOTNLM
OT  - aqueous humor
OT  - extracellular vesicles
OT  - ocular liquid biopsy
OT  - pediatric ocular disease
OT  - single vesicle analysis
COIS- DISCLOSURE STATEMENT Drs. Berry, Xu have filed a provisional patent application 
      entitled, Aqueous Humor Cell Free DNA for Diagnostic and Prognostic Evaluation of 
      Ophthalmic Disease. Otherwise, the authors declare no potential conflicts of 
      interest.
EDAT- 2022/11/08 06:00
MHDA- 2022/11/08 06:01
PMCR- 2022/11/03
CRDT- 2022/11/07 04:49
PHST- 2022/11/07 04:49 [entrez]
PHST- 2022/11/08 06:00 [pubmed]
PHST- 2022/11/08 06:01 [medline]
PHST- 2022/11/03 00:00 [pmc-release]
AID - e36 [pii]
AID - 10.1002/jex2.36 [doi]
PST - ppublish
SO  - J Extracell Biol. 2022 Apr;1(4):e36. doi: 10.1002/jex2.36. Epub 2022 Apr 20.