PMID- 36341061
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221108
IS  - 0972-8988 (Print)
IS  - 2231-0916 (Electronic)
IS  - 0972-8988 (Linking)
VI  - 15
IP  - 9
DP  - 2022 Sep
TI  - Genotypic characterization of mecA gene and antibiogram profile of 
      coagulase-negative staphylococci in subclinical mastitic cows.
PG  - 2186-2191
LID - 10.14202/vetworld.2022.2186-2191 [doi]
AB  - BACKGROUND AND AIM: Coagulase-negative staphylococci (CNS) are becoming the major 
      cause of clinical and subclinical bovine mastitis around the world. This study 
      aims to estimate the prevalence, antibiogram, and frequency of the 
      methicillin-resistant (MR) (mecA) gene in CNS collected from cows with 
      subclinical mastitis. MATERIALS AND METHODS: Thirty-four milk samples were 
      collected from 20 cows. Fifteen subclinical mastitis samples (~44.12%) were 
      identified as CNS isolates. The Vitek2 compact system method was employed for the 
      identification of the species. Furthermore, antibiotic sensitivity tests were 
      performed against 10 different antibiotics for CNS strains. The mecA gene from 
      isolated CNS was detected by conventional polymerase chain reaction (PCR). 
      RESULTS: Staphylococcus haemolyticus was the most predominant isolated species 
      with an incidence of 33.3% (5/15 isolates), followed by 26.7% for Staphylococcus 
      sciuri and Staphylococcus vitamins (4/15 isolates), and 13.3% for Staphylococcus 
      vitulinus (2/15 isolates), respectively. The highest resistance rates were 
      determined to be 40% (6/15 isolates) against penicillin and oxacillin (OX), 33.3% 
      (5/15 isolates) against clindamycin, 13% (2/15 isolates) against chloramphenicol, 
      amoxicillin, and erythromycin, and 5% (1/15 isolates) against ciprofloxacin, 
      respectively. The results revealed that the isolates were resistant to one or 
      more antimicrobial agents, with five isolates displaying multiple antimicrobial 
      resistance. Furthermore, the results exhibit that all CNS isolates had the mecA 
      gene at 310 bp with a 100% frequency. Moreover, for detecting MR isolates, there 
      are significant discrepancies between phenotypic and genotypic approaches, and 
      only 6/15 CNS isolates phenotypically demonstrated OX resistance. CONCLUSION: The 
      results emphasize the necessity of frequent monitoring of phenotypic and 
      genotypic profiles of CNS isolates to ensure effective control measures and the 
      prevention of multidrug resistance strain evolution.
CI  - Copyright: (c) Ibrahim, et al.
FAU - Ibrahim, Eman S
AU  - Ibrahim ES
AD  - Department of Microbiology and Immunology, National Research Centre, Giza, Egypt.
FAU - Dorgham, Sohad M
AU  - Dorgham SM
AD  - Department of Microbiology and Immunology, National Research Centre, Giza, Egypt.
FAU - Mansour, Asmaa S
AU  - Mansour AS
AD  - Department of Microbiology and Immunology, National Research Centre, Giza, Egypt.
FAU - Abdalhamed, Abeer M
AU  - Abdalhamed AM
AD  - Department of Parasitology and Animals Diseases, National Research Centre, Giza, 
      Egypt.
FAU - Khalaf, Doaa D
AU  - Khalaf DD
AD  - Department of Microbiology and Immunology, National Research Centre, Giza, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20220915
PL  - India
TA  - Vet World
JT  - Veterinary world
JID - 101504872
PMC - PMC9631366
OTO - NOTNLM
OT  - coagulase-negative staphylococci
OT  - cows
OT  - mecA gene
OT  - polymerase chain reaction
OT  - subclinical mastitis
COIS- The authors declare that they have no competing interests.
EDAT- 2022/11/08 06:00
MHDA- 2022/11/08 06:01
PMCR- 2022/09/01
CRDT- 2022/11/07 05:11
PHST- 2022/05/19 00:00 [received]
PHST- 2022/07/26 00:00 [accepted]
PHST- 2022/11/07 05:11 [entrez]
PHST- 2022/11/08 06:00 [pubmed]
PHST- 2022/11/08 06:01 [medline]
PHST- 2022/09/01 00:00 [pmc-release]
AID - Vetworld-15-2186 [pii]
AID - 10.14202/vetworld.2022.2186-2191 [doi]
PST - ppublish
SO  - Vet World. 2022 Sep;15(9):2186-2191. doi: 10.14202/vetworld.2022.2186-2191. Epub 
      2022 Sep 15.