PMID- 36341359
OWN - NLM
STAT- MEDLINE
DCOM- 20221108
LR  - 20221109
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 13
DP  - 2022
TI  - Transcriptomics and quantitative proteomics reveal changes after second 
      stimulation of bone marrow-derived macrophages from lupus-prone MRL/lpr mice.
PG  - 1004232
LID - 10.3389/fimmu.2022.1004232 [doi]
LID - 1004232
AB  - Innate immune memory can cause the occurrence and exacerbation of autoimmune 
      diseases, and it is as well as being strongly associated with the pathogenesis of 
      systemic lupus erythematosus (SLE), however, the specific mechanism remains to be 
      further studied. We learned that IFN-gamma stimulation generated innate immune memory 
      in bone marrow-derived macrophages (BMDMs) and activated memory 
      interferon-stimulated genes (ISGs). This research used IFN-gamma and 
      lipopolysaccharide (LPS) to treat BMDMs with lupus-prone MRL/lpr mice and showed 
      that particular memory ISGs were substantially elevated in prestimulated 
      macrophages. In order to identify the differentially expressed genes (DEGs), 
      researchers turned to RNA-seq. GO and KEGG analysis showed that up-regulated DEGs 
      were enriched in defense and innate immune responses, and were related to the 
      expression of pattern recognition receptors (PRRs)-related pathways in 
      macrophages. TMT-based proteome analysis revealed differentially expressed 
      proteins (DEPs) up-regulated in BMDMs were abundant in metabolic pathways such as 
      glucose metabolism. Our study found that after the secondary stimulation of 
      MRL/lpr mice, the expression of PRRs in innate immune cells was changed, and 
      IFN-related pathways were activated to release a large number of ISGs to promote 
      the secondary response. At the same time, related metabolic modes such as 
      glycolysis were enhanced, and epigenetic changes may occur. Therefore, SLE is 
      brought on, maintained, and worsened by a variety of factors that work together 
      to produce innate immune memory.
CI  - Copyright (c) 2022 Chen, Wu, Wang, Li, Shen, Zhao, Ozato and Li.
FAU - Chen, Keyue
AU  - Chen K
AD  - Key Laboratory of Chinese Medicine Rheumatology of Zhejiang Province, School of 
      Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Wu, Tiyun
AU  - Wu T
AD  - Division of Developmental Biology, National Institute of Child Health and Human 
      Development, National Institutes of Health, Bethesda, MD, United States.
FAU - Wang, Danyan
AU  - Wang D
AD  - Key Laboratory of Chinese Medicine Rheumatology of Zhejiang Province, School of 
      Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Li, Rong
AU  - Li R
AD  - Key Laboratory of Chinese Medicine Rheumatology of Zhejiang Province, School of 
      Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Shen, Xiangfeng
AU  - Shen X
AD  - Key Laboratory of Chinese Medicine Rheumatology of Zhejiang Province, School of 
      Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Zhao, Ting
AU  - Zhao T
AD  - Key Laboratory of Chinese Medicine Rheumatology of Zhejiang Province, School of 
      Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Ozato, Keiko
AU  - Ozato K
AD  - Division of Developmental Biology, National Institute of Child Health and Human 
      Development, National Institutes of Health, Bethesda, MD, United States.
FAU - Li, Rongqun
AU  - Li R
AD  - Key Laboratory of Chinese Medicine Rheumatology of Zhejiang Province, School of 
      Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
LA  - eng
GR  - ZIA HD001310/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20221019
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
SB  - IM
MH  - Mice
MH  - Animals
MH  - Mice, Inbred MRL lpr
MH  - *Transcriptome
MH  - Proteomics
MH  - *Lupus Erythematosus, Systemic
MH  - Macrophages/pathology
PMC - PMC9627492
OTO - NOTNLM
OT  - MRL/lpr mice
OT  - RNA-seq
OT  - innate immune memory
OT  - proteome
OT  - systemic lupus erythematosus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest. The reviewer LL declared a shared parent affiliation with 
      the authors KC, DW, RL, XS, TZ and RL to the handling editor at the time of 
      review.
EDAT- 2022/11/08 06:00
MHDA- 2022/11/09 06:00
PMCR- 2022/01/01
CRDT- 2022/11/07 05:17
PHST- 2022/07/27 00:00 [received]
PHST- 2022/09/27 00:00 [accepted]
PHST- 2022/11/07 05:17 [entrez]
PHST- 2022/11/08 06:00 [pubmed]
PHST- 2022/11/09 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2022.1004232 [doi]
PST - epublish
SO  - Front Immunol. 2022 Oct 19;13:1004232. doi: 10.3389/fimmu.2022.1004232. 
      eCollection 2022.