PMID- 36343898
OWN - NLM
STAT- MEDLINE
DCOM- 20221125
LR  - 20221226
IS  - 1521-7035 (Electronic)
IS  - 1521-6616 (Linking)
VI  - 245
DP  - 2022 Dec
TI  - Single-cell transcriptome reveals immunopathological cell composition of skin 
      lesions in subacute cutaneous lupus erythematosus.
PG  - 109172
LID - S1521-6616(22)00253-4 [pii]
LID - 10.1016/j.clim.2022.109172 [doi]
AB  - Subacute cutaneous lupus erythematosus (SCLE) is a clinical subtype of cutaneous 
      lupus erythematosus with psoriatic-like or annular papules with scaly erythemas, 
      the pathological mechanism of which is poorly understood. To investigate the 
      immune pathogenesis of SCLE, we performed single-cell RNA sequencing (scRNA-seq) 
      of SCLE skin lesions and integrated the scRNA-seq data from skin tissues of 
      healthy controls (HCs). Our results identified expanded fibroblasts and 
      keratinocytes subtypes, abnormally activated lymphocyte and inflammatory M1 
      macrophages in SCLE. In SCLE, stromal cells, such as keratinocytes and 
      fibroblasts, showed enhanced chemotactic functions for recruiting immune cells. 
      Importantly, interferon-related genes were identified as key intermediate genes 
      in the potential trajectory of fibroblasts, keratinocytes, and B cells from HCs 
      to SCLE. Our investigation provides a comprehensive description of cell 
      composition in SCLE and highlights several important clues for understanding the 
      pathogenesis of SCLE.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Zheng, Meiling
AU  - Zheng M
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Medical Epigenomics, Changsha 410011, China; Research 
      Unit of Key Technologies of Diagnosis and Treatment for Immune-related Skin 
      Diseases, Chinese Academy of Medical Sciences, Changsha 410011, China.
FAU - Hu, Zhi
AU  - Hu Z
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Medical Epigenomics, Changsha 410011, China; Research 
      Unit of Key Technologies of Diagnosis and Treatment for Immune-related Skin 
      Diseases, Chinese Academy of Medical Sciences, Changsha 410011, China.
FAU - Zhou, Wenhui
AU  - Zhou W
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Medical Epigenomics, Changsha 410011, China; Research 
      Unit of Key Technologies of Diagnosis and Treatment for Immune-related Skin 
      Diseases, Chinese Academy of Medical Sciences, Changsha 410011, China.
FAU - Kong, Yi
AU  - Kong Y
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Medical Epigenomics, Changsha 410011, China; Research 
      Unit of Key Technologies of Diagnosis and Treatment for Immune-related Skin 
      Diseases, Chinese Academy of Medical Sciences, Changsha 410011, China.
FAU - Wu, Ruifang
AU  - Wu R
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Medical Epigenomics, Changsha 410011, China; Research 
      Unit of Key Technologies of Diagnosis and Treatment for Immune-related Skin 
      Diseases, Chinese Academy of Medical Sciences, Changsha 410011, China.
FAU - Zhang, Bo
AU  - Zhang B
AD  - Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Nanjing 210042, China.
FAU - Long, Hai
AU  - Long H
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Medical Epigenomics, Changsha 410011, China; Research 
      Unit of Key Technologies of Diagnosis and Treatment for Immune-related Skin 
      Diseases, Chinese Academy of Medical Sciences, Changsha 410011, China.
FAU - Jia, Sujie
AU  - Jia S
AD  - Department of Pharmacy, The Third Xiangya Hospital, Central South University, 
      Changsha 410011, China.
FAU - Lu, Qianjin
AU  - Lu Q
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Medical Epigenomics, Changsha 410011, China; Research 
      Unit of Key Technologies of Diagnosis and Treatment for Immune-related Skin 
      Diseases, Chinese Academy of Medical Sciences, Changsha 410011, China; Institute 
      of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical 
      College, Nanjing 210042, China.
FAU - Zhao, Ming
AU  - Zhao M
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Medical Epigenomics, Changsha 410011, China; Research 
      Unit of Key Technologies of Diagnosis and Treatment for Immune-related Skin 
      Diseases, Chinese Academy of Medical Sciences, Changsha 410011, China; Institute 
      of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical 
      College, Nanjing 210042, China. Electronic address: zhaoming307@csu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221105
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
SB  - IM
MH  - Humans
MH  - *Transcriptome
MH  - *Lupus Erythematosus, Cutaneous/genetics
MH  - Skin
MH  - Keratinocytes
MH  - B-Lymphocytes/pathology
OTO - NOTNLM
OT  - Immune cells
OT  - Interferon-related genes
OT  - SCLE
OT  - Single cell RNA sequencing
COIS- Declaration of Competing Interest All authors declare that there are no conflicts 
      of interest.
EDAT- 2022/11/08 06:00
MHDA- 2022/11/26 06:00
CRDT- 2022/11/07 19:27
PHST- 2022/08/17 00:00 [received]
PHST- 2022/10/14 00:00 [revised]
PHST- 2022/10/25 00:00 [accepted]
PHST- 2022/11/08 06:00 [pubmed]
PHST- 2022/11/26 06:00 [medline]
PHST- 2022/11/07 19:27 [entrez]
AID - S1521-6616(22)00253-4 [pii]
AID - 10.1016/j.clim.2022.109172 [doi]
PST - ppublish
SO  - Clin Immunol. 2022 Dec;245:109172. doi: 10.1016/j.clim.2022.109172. Epub 2022 Nov 
      5.