PMID- 36344553
OWN - NLM
STAT- MEDLINE
DCOM- 20221109
LR  - 20230105
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Nov 7
TI  - MUC7 VNTR polymorphism and association with bronchial asthma in Egyptian 
      children.
PG  - 18910
LID - 10.1038/s41598-022-21631-4 [doi]
LID - 18910
AB  - Overproduction of mucins in the airways donates largely to airway blockage in 
      asthma patients. Glycoprotein MUC7 plays a role in the clearance of bacteria and 
      has anti-candidacidal criteria. Our goal was to investigate the association 
      between the MUC7 variable number of tandem repeats (VNTR) polymorphism and 
      bronchial asthma among Egyptian children. The MUC7 VNTR polymorphism was 
      investigated among 100 children with bronchial asthma and 100 healthy controls 
      using polymerase chain reaction (PCR) method. Serum levels of immunoglobulin E 
      (IgE), tumor necrosis factor-alpha (TNF-alpha), and transforming growth factor-beta1 
      (TGF-beta1) were assessed by enzyme-linked immunosorbent assay (ELISA) technique. 
      The frequencies of 6*5 genotype, 5*5 genotype, (6*5 + 5*5) genotypes, and MUC7*5 
      allele of the MUC7 VNTR variant were significantly lower among asthmatic patients 
      than controls (p < 0.015, OR = 0.39, 95% CI = 0.19-0.81; p = 0.03, OR = 0.18, 95% 
      CI = 0.04-0.86; p < 0.001, OR = 0.29, 95% CI = 0.15-0.58; p < 0.001, OR = 0.3, 
      95% CI = 0.17-0.55, respectively). The (6*5 + 5*5) genotypes of the MUC7 VNTR 
      variant were not associated with the clinical manifestations and serum levels of 
      IgE, TNF-alpha, and TGF-beta1 among asthmatic patients (p > 0.05). In conclusion, the 
      (6*5 + 5*5) genotypes of the MUC7 VNTR variant may have a protective role for 
      bronchial asthma in Egyptian children.
CI  - (c) 2022. The Author(s).
FAU - Saad, Entsar A
AU  - Saad EA
AUID- ORCID: 0000-0001-6477-8098
AD  - Chemistry Department, Faculty of Science, Damietta University, Damietta, 34517, 
      Egypt. entsarsaad@gmail.com.
FAU - Elsaid, Afaf M
AU  - Elsaid AM
AD  - Genetics Unit, Children Hospital, Mansoura University, Mansoura, Egypt.
FAU - Shoaib, Rasha M S
AU  - Shoaib RMS
AD  - Food and Dairy Sciences and Technology Department, Faculty of Environmental 
      Agricultural Sciences, Arish University, Arish, North Sinai, Egypt.
FAU - Megahed, Khaled F
AU  - Megahed KF
AD  - Department of Pediatrics, Faculty of Medicine, Mansoura University, Mansoura, 
      Egypt.
FAU - Elsharawy, Amal N
AU  - Elsharawy AN
AD  - Chemistry Department, Faculty of Science, Damietta University, Damietta, 34517, 
      Egypt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221107
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (MUC7 protein, human)
RN  - 0 (Mucins)
RN  - 0 (Salivary Proteins and Peptides)
SB  - IM
MH  - Child
MH  - Humans
MH  - *Polymorphism, Genetic
MH  - Transforming Growth Factor beta1/genetics
MH  - Minisatellite Repeats
MH  - Tumor Necrosis Factor-alpha/genetics
MH  - Egypt
MH  - *Asthma/genetics
MH  - Genotype
MH  - Immunoglobulin E/genetics
MH  - Genetic Predisposition to Disease
MH  - Mucins/genetics
MH  - Salivary Proteins and Peptides/genetics
PMC - PMC9640678
COIS- The authors declare no competing interests.
EDAT- 2022/11/08 06:00
MHDA- 2022/11/10 06:00
PMCR- 2022/11/07
CRDT- 2022/11/07 23:21
PHST- 2022/01/28 00:00 [received]
PHST- 2022/09/29 00:00 [accepted]
PHST- 2022/11/07 23:21 [entrez]
PHST- 2022/11/08 06:00 [pubmed]
PHST- 2022/11/10 06:00 [medline]
PHST- 2022/11/07 00:00 [pmc-release]
AID - 10.1038/s41598-022-21631-4 [pii]
AID - 21631 [pii]
AID - 10.1038/s41598-022-21631-4 [doi]
PST - epublish
SO  - Sci Rep. 2022 Nov 7;12(1):18910. doi: 10.1038/s41598-022-21631-4.