PMID- 36346897
OWN - NLM
STAT- MEDLINE
DCOM- 20221123
LR  - 20221208
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 94
IP  - 46
DP  - 2022 Nov 22
TI  - Ultrasensitive Fluorescence Detection and Imaging of MicroRNA in Cells Based on a 
      Hyperbranched RCA-Assisted Multiposition SDR Signal Amplification Strategy.
PG  - 16237-16245
LID - 10.1021/acs.analchem.2c04037 [doi]
AB  - Herein, an innovative fluorescent sensor was courageously empoldered for precise 
      and ultrasensitive detection and imaging of target miRNA-21 through the agency of 
      a dextrous target-motivated polymerization/nicking DNA nanomachineries based on a 
      hyperbranched rolling circle amplification (HB-RCA)-assisted multiposition strand 
      displacement reaction (SDR) signal amplification approach. Impressively, the 
      ingenious technique not only realized target recycling via polymerization/nicking 
      DNA nanomachineries but also involved HB-RCA amplification induced by the 
      released transformation target as the repeated signal amplification. Most 
      importantly, HB-RCA was firstly exploited to remarkably increase the local 
      concentration and collision efficiency of the templates and primers, which could 
      simultaneously generate multiple repeated DNA sequences as initiators to supply 
      substantial banding positions for SDR, removing the massive 
      fluorescence-resonance-energy-transfer (FRET) DNA duplexes from the repeated DNA 
      sequences to remarkably avert the self-quenching of the fluorescence signal due 
      to self-aggregation caused by the winding of the HB-RCA products, thereby leading 
      to a conspicuously improved signal amplification multiplier. As proof of concept, 
      an ingenious technique effectively and accurately distinguished target miRNA-21 
      even with a tiny change in cells compared to the conventional fluorescence in 
      situ hybridization (FISH) approach. Moreover, the proposed fluorescent method 
      apparently discriminated drug-manipulative miRNA expression level abnormities. 
      Therefore, the proposed cascade nucleic acid amplification strategy could provide 
      an epigamic avenue for ultrasensitive imaging of diverse biomarkers, which help 
      researchers to better study the tumor mechanism, thereby unambiguously increasing 
      cancer cure rates and reducing the risk of recurrence.
FAU - Yang, Zezhou
AU  - Yang Z
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University, Chongqing 400715, PR China.
FAU - Guo, Yu
AU  - Guo Y
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University, Chongqing 400715, PR China.
FAU - Zhou, Jie
AU  - Zhou J
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University, Chongqing 400715, PR China.
FAU - Liu, Fang
AU  - Liu F
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University, Chongqing 400715, PR China.
FAU - Liang, Wenbin
AU  - Liang W
AUID- ORCID: 0000-0002-9086-4974
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University, Chongqing 400715, PR China.
FAU - Chai, Yaqin
AU  - Chai Y
AUID- ORCID: 0000-0003-4392-9592
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University, Chongqing 400715, PR China.
FAU - Li, Zhaohui
AU  - Li Z
AUID- ORCID: 0000-0003-3946-0656
AD  - Henan Joint International Research Laboratory of Green Construction of Functional 
      Molecules and Their Bioanalytical Applications, College of Chemistry, Zhengzhou 
      University, Zhengzhou 450001, P. R. China.
FAU - Yuan, Ruo
AU  - Yuan R
AUID- ORCID: 0000-0003-3664-6236
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University, Chongqing 400715, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221108
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (MicroRNAs)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *MicroRNAs/genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleic Acid Amplification Techniques/methods
MH  - DNA/genetics
MH  - Spectrometry, Fluorescence/methods
MH  - Limit of Detection
MH  - *Biosensing Techniques/methods
EDAT- 2022/11/09 06:00
MHDA- 2022/11/24 06:00
CRDT- 2022/11/08 14:22
PHST- 2022/11/09 06:00 [pubmed]
PHST- 2022/11/24 06:00 [medline]
PHST- 2022/11/08 14:22 [entrez]
AID - 10.1021/acs.analchem.2c04037 [doi]
PST - ppublish
SO  - Anal Chem. 2022 Nov 22;94(46):16237-16245. doi: 10.1021/acs.analchem.2c04037. 
      Epub 2022 Nov 8.