PMID- 36349569
OWN - NLM
STAT- MEDLINE
DCOM- 20221206
LR  - 20221206
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 173
IP  - Pt A
DP  - 2022 Dec
TI  - Antibacterial mechanism of forsythoside A against Pseudomonas syringae pv. 
      actinidiae.
PG  - 105858
LID - S0882-4010(22)00471-5 [pii]
LID - 10.1016/j.micpath.2022.105858 [doi]
AB  - In this study, we investigated the antibacterial mechanism of forsythoside A 
      against the kiwifruit canker pathogen, which provided the theoretical basis for 
      the prevention and control of canker disease and the development of plant-based 
      fungicides. The pathogenic bacteria were isolated from kiwifruit diseased tissues 
      and the specific primers Psa_A1 F2 and Psa_A1 R1 were used for preliminary 
      identification. Four pairs of housekeeping genes, including gapA, gltA, gyrB, and 
      rpoD, were used for polygenic typing identification. The inhibition effect of 
      forsythoside A on Psa was evaluated by the filter paper bacteriostasis method. 
      The minimum inhibitory concentration (MIC) and minimum bactericidal concentration 
      (MBC) of Psa were determined by the 96-well plate absorbance and colony counts. 
      The changes in Psa biofilm formation, motility, IAA synthesis, iron utilization, 
      and respiratory chain dehydrogenase activity were determined. The Psa morphology 
      was observed by Scanning electron microscope (SEM) and transmission electron 
      microscope (TEM). The expression of some virulence genes was analyzed by qPCR. 
      The results showed that the pathogen was Pseudomonas syringae pv. 
      actinidiae(Psa). The inhibitory effect of forsythoside A on Psa was positively 
      correlated with its concentration. while the MIC and MBC were 2.0 and 5.0 mg/mL, 
      respectively. The biofilm formation and motility of Psa were not only obviously 
      inhibited, but also the substance and energy metabolism were interfered, while 
      obvious deformity and rupture of the cells were occurred in Psa Bacteria. In 
      addition, The transcription of the Psa pathogenic genes was affected. The 
      infection investigation of kiwifruit leaves indicated that forsythiaside A 
      inhibits Psa pathogenicity and had a protective effect. This study concluded that 
      forsythoside A has a certain control effect on kiwifruit canker, and has the 
      potentiality to be developed as a novel plant fungicide.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Pei, Hurong
AU  - Pei H
AD  - Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, 
      College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610065, China.
FAU - Lu, Mingxiu
AU  - Lu M
AD  - Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, 
      College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610065, China.
FAU - Long, Li
AU  - Long L
AD  - Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, 
      College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610065, China.
FAU - Long, Zhangfu
AU  - Long Z
AD  - Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, 
      College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610065, China. 
      Electronic address: Lzf0028@163.com.
LA  - eng
PT  - Journal Article
DEP - 20221029
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - OUH5BQ893P (forsythiaside)
RN  - 0 (Glycosides)
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - *Pseudomonas syringae/genetics
MH  - Plant Diseases/microbiology
MH  - Glycosides/pharmacology
MH  - *Actinidia
MH  - Anti-Bacterial Agents/pharmacology
OTO - NOTNLM
OT  - Antibacterial mechanism
OT  - Forsythoside A
OT  - Kiwifruit canker
OT  - qRT-PCR
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/11/10 06:00
MHDA- 2022/12/07 06:00
CRDT- 2022/11/09 04:03
PHST- 2022/09/02 00:00 [received]
PHST- 2022/10/24 00:00 [revised]
PHST- 2022/10/25 00:00 [accepted]
PHST- 2022/11/10 06:00 [pubmed]
PHST- 2022/12/07 06:00 [medline]
PHST- 2022/11/09 04:03 [entrez]
AID - S0882-4010(22)00471-5 [pii]
AID - 10.1016/j.micpath.2022.105858 [doi]
PST - ppublish
SO  - Microb Pathog. 2022 Dec;173(Pt A):105858. doi: 10.1016/j.micpath.2022.105858. 
      Epub 2022 Oct 29.