PMID- 36350155
OWN - NLM
STAT- MEDLINE
DCOM- 20230215
LR  - 20230215
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 38
IP  - 2
DP  - 2023 Feb
TI  - Apigenin attenuates inflammatory response in allergic rhinitis mice by inhibiting 
      the TLR4/MyD88/NF-kappaB signaling pathway.
PG  - 253-265
LID - 10.1002/tox.23699 [doi]
AB  - BACKGROUND: Allergic rhinitis (AR) is an immunoglobulin E (IgE)-mediated immune 
      inflammatory response that mainly affects the nasal mucosa. Currently, there is 
      evidence that apigenin, as a flavonoid, has anti-allergic potential. 
      MATERIAL/METHODS: In vitro, compound 48/80 and lipopolysaccharide (LPS) were used 
      to induce mast cell activation and inflammation in HMC-1 cells. In vivo, 
      ovalbumin (OVA) induced and stimulated AR in BALB/c mice. ELISA was used to 
      detect the contents of beta-hexosaminidase, histamine, eosinophil cationic protein 
      (ECP), OVA-specific IgE, IgG1, and IgG2a, inflammatory factors in cells and mouse 
      serum. Cell viability and apoptosis were measured with MTT and flow cytometry. 
      Toll like receptor 4 (TLR4)/myeloid differentiation factor88 (MyD88)/Nuclear 
      transcription factor-kappaB (NF-kappaB) pathway-related proteins in cells and mouse nasal 
      mucosa tissues were analyzed with Western blotting. The levels of Th1 (IFN-gamma) and 
      Th2 (IL-4, IL-5, and IL-13) cytokines and Th1 (T-bet) and Th2 (GATA-3) specific 
      transcription factors were also assessed. The ratio of Th1 (CD4(+) IFN-gamma(+) ) / 
      Th2 (CD4(+) IL-4(+) ) cells in mouse peripheral blood mononuclear cells was 
      evaluated by flow cytometry. RESULTS: Apigenin significantly inhibited compound 
      48/80-induced secretion of beta-hexosaminidase and histamine. Apigenin blocked 
      LPS-induced decrease in cell viability and increase in cell apoptosis and 
      inflammatory cytokine secretion by suppressing the activity of the 
      TLR4/MyD88/NF-kappaB pathway. Apigenin treatment reduced the levels of OVA-specific 
      IgE, IgG1 and IgG2a as well as beta-hexosaminidase, histamine and ECP levels in 
      mouse serum. Moreover, administration with apigenin decreased Th2 cytokine and 
      transcription factor levels and increased Th1 cytokine and transcription factor 
      levels, and promoted the ratio of Th1/Th2 cells in AR mice. Additionally, 
      apigenin significantly alleviated nasal symptoms and nasal eosinophil 
      infiltration in AR mice. CONCLUSIONS: Apigenin alleviates the inflammatory 
      response of allergic rhinitis by inhibiting the activity of the TLR4/MyD88/NF-kappaB 
      signaling pathway.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Li, Huajing
AU  - Li H
AD  - Department of Otolaryngology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Zhang, Hongmei
AU  - Zhang H
AD  - Department of Otolaryngology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Zhao, Hua
AU  - Zhao H
AUID- ORCID: 0000-0002-4627-247X
AD  - Department of Pharmacy, Affiliated Hospital of Medical College of Xi'an Jiaotong 
      University, Shaanxi Provincial Cancer Hospital, Xi'an, China.
LA  - eng
GR  - The Basic research plan of Natural Science in Shaanxi Province (2020JM-382) in 
      China/
PT  - Journal Article
DEP - 20221109
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - 7V515PI7F6 (Apigenin)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
RN  - 0 (Cytokines)
RN  - 820484N8I3 (Histamine)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Immunoglobulin G)
RN  - 207137-56-2 (Interleukin-4)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 9006-59-1 (Ovalbumin)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Apigenin/pharmacology/therapeutic use
MH  - beta-N-Acetylhexosaminidases/metabolism
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Histamine/toxicity
MH  - Immunoglobulin E
MH  - Immunoglobulin G/toxicity/metabolism
MH  - Interleukin-4
MH  - Leukocytes, Mononuclear/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Mice, Inbred BALB C
MH  - *Myeloid Differentiation Factor 88/metabolism
MH  - *NF-kappa B/metabolism
MH  - Ovalbumin/pharmacology
MH  - *Rhinitis, Allergic/chemically induced/drug therapy
MH  - Signal Transduction
MH  - Th2 Cells
MH  - *Toll-Like Receptor 4/metabolism
OTO - NOTNLM
OT  - HMC-1 mast cells
OT  - TLR4/MyD88/NF-kappaB pathway
OT  - allergic rhinitis
OT  - apigenin
OT  - inflammatory response
EDAT- 2022/11/10 06:00
MHDA- 2023/01/18 06:00
CRDT- 2022/11/09 09:04
PHST- 2022/10/10 00:00 [revised]
PHST- 2021/08/03 00:00 [received]
PHST- 2022/10/13 00:00 [accepted]
PHST- 2022/11/10 06:00 [pubmed]
PHST- 2023/01/18 06:00 [medline]
PHST- 2022/11/09 09:04 [entrez]
AID - 10.1002/tox.23699 [doi]
PST - ppublish
SO  - Environ Toxicol. 2023 Feb;38(2):253-265. doi: 10.1002/tox.23699. Epub 2022 Nov 9.