PMID- 36351839
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221123
IS  - 1226-2560 (Print)
IS  - 2093-8144 (Electronic)
IS  - 1226-2560 (Linking)
VI  - 31
IP  - 5
DP  - 2022 Oct 31
TI  - Distribution of Neuroglobin in Pericytes is Associated with Blood-Brain Barrier 
      Leakage against Cerebral Ischemia in Mice.
PG  - 289-298
LID - 10.5607/en22001 [doi]
AB  - With emerging data on the various functions of neuroglobin (Ngb), such as 
      neuroprotection and neurogenesis, we investigated the role of Ngb in the 
      neurovascular unit (NVU) of the brain. To study the distribution and function of 
      Ngb after cerebral ischemia, transient middle cerebral artery occlusion (tMCAO) 
      was performed in mice. Brain immunostaining and fluorescence-activated cell 
      sorting were used to analyze the role of Ngb according to the location and cell 
      type. In normal brain tissue, it was observed that Ngb was distributed not only 
      in neurons but also around the brain's blood vessels. Interestingly, Ngb was 
      largely expressed in platelet-derived growth factor receptor beta (PDGFRbeta)-positive 
      pericytes in the NVU. After tMCAO, Ngb levels were significantly decreased in the 
      core of the infarct, and Ngb and PDGFRbeta-positive pericytes were detached from the 
      vasculature. In contrast, in the penumbra of the infarct, PDGFRbeta-positive 
      pericytes expressing Ngb were increased compared with that in the core of the 
      infarct. Moreover, the cerebral blood vessels, which have Ngb-positive PDGFRbeta 
      pericytes, showed reduced blood-brain barrier (BBB) leakage after tMCAO. It 
      showed that Ngb-positive PDGFRbeta pericytes stayed around the endothelial cells and 
      reduced the BBB leakage in the NVU. Our results indicate that Ngb may play a role 
      in attenuating BBB leakage in part by its association with PDGFRbeta. In this study, 
      the distribution and function of Ngb in the pericytes of the cerebrovascular 
      system have been elucidated, which contributes to the treatment of stroke through 
      a new function of Ngb.
FAU - Kim, Yeojin
AU  - Kim Y
AD  - Department of Pharmacology, College of Pharmacy, Drug Information Research 
      Institute, Muscle Physiome Research Center, Sookmyung Women's University, Seoul 
      04310, Korea.
FAU - Kim, Mingee
AU  - Kim M
AD  - Department of Pharmacology, College of Pharmacy, Drug Information Research 
      Institute, Muscle Physiome Research Center, Sookmyung Women's University, Seoul 
      04310, Korea.
FAU - Kim, So-Dam
AU  - Kim SD
AD  - Department of Pharmacology, College of Pharmacy, Drug Information Research 
      Institute, Muscle Physiome Research Center, Sookmyung Women's University, Seoul 
      04310, Korea.
FAU - Yoon, Naeun
AU  - Yoon N
AD  - Department of Pharmacology, College of Pharmacy, Drug Information Research 
      Institute, Muscle Physiome Research Center, Sookmyung Women's University, Seoul 
      04310, Korea.
FAU - Wang, Xiaoying
AU  - Wang X
AD  - Clinical Neuroscience Research Center, Department of Neurosurgery and Neurology, 
      Tulane University School of Medicine, New Orleans, LA 70112, USA.
FAU - Bae, Gyu-Un
AU  - Bae GU
AD  - Department of Pharmacology, College of Pharmacy, Drug Information Research 
      Institute, Muscle Physiome Research Center, Sookmyung Women's University, Seoul 
      04310, Korea.
FAU - Song, Yun Seon
AU  - Song YS
AD  - Department of Pharmacology, College of Pharmacy, Drug Information Research 
      Institute, Muscle Physiome Research Center, Sookmyung Women's University, Seoul 
      04310, Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Exp Neurobiol
JT  - Experimental neurobiology
JID - 101278187
PMC - PMC9659490
OTO - NOTNLM
OT  - Blood-brain barrier
OT  - Ischemic stroke
OT  - Neuroglobin
OT  - Pericytes
EDAT- 2022/11/10 06:00
MHDA- 2022/11/10 06:01
PMCR- 2022/10/31
CRDT- 2022/11/09 21:42
PHST- 2021/12/14 00:00 [received]
PHST- 2022/09/27 00:00 [revised]
PHST- 2022/10/09 00:00 [accepted]
PHST- 2022/11/09 21:42 [entrez]
PHST- 2022/11/10 06:00 [pubmed]
PHST- 2022/11/10 06:01 [medline]
PHST- 2022/10/31 00:00 [pmc-release]
AID - en22001 [pii]
AID - en-31-5-289 [pii]
AID - 10.5607/en22001 [doi]
PST - ppublish
SO  - Exp Neurobiol. 2022 Oct 31;31(5):289-298. doi: 10.5607/en22001.