PMID- 36352998
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221111
IS  - 2249-4863 (Print)
IS  - 2278-7135 (Electronic)
IS  - 2249-4863 (Linking)
VI  - 11
IP  - 8
DP  - 2022 Aug
TI  - Association of -675 4G/5G PAI-1 and -2518A/G MCP-1 genetic polymorphisms with 
      polycystic ovary syndrome in Kashmiri women: A case control study.
PG  - 4743-4752
LID - 10.4103/jfmpc.jfmpc_1916_21 [doi]
AB  - BACKGROUND: Polycystic ovarian syndrome (PCOS) is a highly prevalent endocrine 
      disorder among females of fertile age. It has been speculated to be associated 
      with low-grade chronic inflammation like other inflammatory response-driven 
      multifactorial illnesses such as diabetes mellitus (DM) and cancer. Monocyte 
      chemoattractant protein-1 (MCP-1) and plasminogen activator inhibitor-1 (PAI-1) 
      are biomarkers of inflammation and endothelial dysfunction, respectively. These 
      have been found to be elevated in PCOS patients. The current research reveals 
      that single nucleotide polymorphisms (SNPs) in their genes are strongly 
      associated with the elevation of these biomarkers. The goal of this study was to 
      see if there was a link between PAI-1 -675 4G/5G and MCP-1 -2518 A/G 
      polymorphisms with the occurrence of PCOS. MATERIAL AND METHOD: This study 
      included 220 PCOS participants and 220 healthy controls. The allele-specific 
      polymerase chain reaction (PCR) and restriction fragment length polymorphism 
      (RFLP) methods were used to investigate PAI-1-675 4G/5G and MCP-1 -2518A/G SNPs, 
      respectively. RESULTS: The -675 4G/5G SNP in the PAI-1 gene was strongly linked 
      to PCOS. The odds ratio (OR) for the 4G/4G genotype was (OR = 3.2; P = 0.001), 
      whereas the OR for the 4G/5G genotype was (OR = 2.39; P = 0.001). The carriers 
      with the 4G/4G and 4G/5G genotypes showed significantly increasing trends in the 
      triglyceride levels (P < 0.05). The genotypic frequency of the -2518 A/G MCP-1 
      SNP differed significantly between the PCOS patients and healthy controls; the GG 
      genotype remained a strong predictor of PCOS (OR = 8.7; P = 0.01) and the AG 
      genotype (OR = 2.40; P = 0.01), indicating an elevated risk of predisposing women 
      to PCOS. There was a significant variation in the glucose 2-h levels between 
      -2518A/G MCP-1 genotypes with AG heterozygous and GG mutant genotype showing 
      increasing trends of glucose 2-h levels (P < 0.05). CONCLUSION: Both PAI-1 -675 
      4G/5G and MCP-1 -2518A/G polymorphisms are associated with predisposition to PCOS 
      and its complications in Kashmiri women.
CI  - Copyright: (c) 2022 Journal of Family Medicine and Primary Care.
FAU - Yousuf, Syed Douhath
AU  - Yousuf SD
AD  - Department of Clinical Biochemistry, Sher-i-Kashmir Institute of Medical Sciences 
      (SKIMS), Jammu and Kashmir, India.
FAU - Ganie, Mohammad Ashraf
AU  - Ganie MA
AD  - Department of Endocinology, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), 
      Jammu and Kashmir, India.
FAU - Mudassar, Syed
AU  - Mudassar S
AD  - Department of Clinical Biochemistry, Sher-i-Kashmir Institute of Medical Sciences 
      (SKIMS), Jammu and Kashmir, India.
FAU - Shafi, Humiara
AU  - Shafi H
AD  - Department of Clinical Biochemistry, University of Kashmir, Jammu and Kashmir, 
      India.
FAU - Ibrahim, Saima
AU  - Ibrahim S
AD  - Department of Clinical Biochemistry, University of Kashmir, Jammu and Kashmir, 
      India.
FAU - Jeelani, Humira
AU  - Jeelani H
AD  - Advanced Centre for Human Genetics, Sher-i-Kashmir Institute of Medical Sciences 
      (SKIMS), Jammu and Kashmir, India.
FAU - Rashid, Gowhar
AU  - Rashid G
AD  - Department of Clinical Biochemistry, Sher-i-Kashmir Institute of Medical Sciences 
      (SKIMS), Jammu and Kashmir, India.
FAU - Zargar, Mohammad A
AU  - Zargar MA
AD  - Department of Biotechnology, Nuner Campus, Central University of Kashmir, Jammu 
      and Kashmir, India.
FAU - Rashid, Fouzia
AU  - Rashid F
AD  - Department of Clinical Biochemistry, University of Kashmir, Jammu and Kashmir, 
      India.
LA  - eng
PT  - Journal Article
DEP - 20220830
PL  - India
TA  - J Family Med Prim Care
JT  - Journal of family medicine and primary care
JID - 101610082
PMC - PMC9638670
OTO - NOTNLM
OT  - Coagulation
OT  - inflammation
OT  - monocyte chemoattractant protein-1
OT  - plasminogen activator inhibitor-1
OT  - polycystic ovary syndrome
OT  - polymorphism
COIS- There are no conflicts of interest.
EDAT- 2022/11/11 06:00
MHDA- 2022/11/11 06:01
PMCR- 2022/08/01
CRDT- 2022/11/10 02:06
PHST- 2021/09/24 00:00 [received]
PHST- 2021/12/21 00:00 [revised]
PHST- 2022/02/17 00:00 [accepted]
PHST- 2022/11/10 02:06 [entrez]
PHST- 2022/11/11 06:00 [pubmed]
PHST- 2022/11/11 06:01 [medline]
PHST- 2022/08/01 00:00 [pmc-release]
AID - JFMPC-11-4743 [pii]
AID - 10.4103/jfmpc.jfmpc_1916_21 [doi]
PST - ppublish
SO  - J Family Med Prim Care. 2022 Aug;11(8):4743-4752. doi: 
      10.4103/jfmpc.jfmpc_1916_21. Epub 2022 Aug 30.