PMID- 36353458
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221111
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - Effects of Danggui Buxue decoction on host gut microbiota and metabolism in GK 
      rats with type 2 diabetes.
PG  - 1029409
LID - 10.3389/fmicb.2022.1029409 [doi]
LID - 1029409
AB  - Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by 
      persistent abnormally elevated blood sugar levels. T2DM affects millions of 
      people and exerts a significant global public health burden. Danggui Buxue 
      decoction (DBD), a classical Chinese herbal formula composed of Astragalus 
      membranaceus (Huangqi) and Angelica sinensis (Danggui), has been widely used in 
      the clinical treatment of diabetes and its complications. However, the effect of 
      DBD on the gut microbiota of individuals with diabetes and its metabolism are 
      still poorly understood. In this study, a T2DM model was established in 
      Goto-Kakizaki (GK) rats, which were then treated with a clinical dose of DBD 
      (4 g/kg) through tube feeding for 6 weeks. Next, we used 16S rRNA sequencing and 
      untargeted metabolomics by liquid chromatography with mass spectrometry (LC-MS) 
      to detect changes in the composition of the microbiota and cecal metabolic 
      products. Our data show that DBD mediates the continuous increase in blood 
      glucose in GK rats, improves insulin sensitivity, reduces expression of 
      inflammatory mediators, and improves systemic oxidative stress. Moreover, DBD 
      also improves microbial diversity (e.g., Romboutsia, Firmicutes, and Bacilli) in 
      the intestines of rats with T2DM. Further, DBD intervention also regulates 
      various metabolic pathways in the gut microbiota, including alanine, aspartate, 
      and glutamate metabolism. In addition, arginine biosynthesis and the isoflavone 
      biosynthesis may be a unique mechanism by which DBD exerts its effects. Taken 
      together, we show that DBD is a promising therapeutic agent that can restore the 
      imbalance found in the gut microbiota of T2DM rats. DBD may modify metabolites in 
      the microbiota to realize its antidiabetic and anti-inflammatory effects.
CI  - Copyright (c) 2022 Wang, Fan, Ge, Li, Zhu, Yin, Xia and Xue.
FAU - Wang, Wen-Kai
AU  - Wang WK
AD  - College of Traditional Chinese Medicine, College of Integrated Chinese and 
      Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
AD  - Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 
      China.
FAU - Fan, Lu
AU  - Fan L
AD  - School of Medicine and Holistic Integrative Medicine, Nanjing University of 
      Chinese Medicine, Nanjing, China.
FAU - Ge, Fan
AU  - Ge F
AD  - College of Traditional Chinese Medicine, College of Integrated Chinese and 
      Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Li, Zihang
AU  - Li Z
AD  - College of Traditional Chinese Medicine, College of Integrated Chinese and 
      Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Zhu, Jingtian
AU  - Zhu J
AD  - College of Traditional Chinese Medicine, College of Integrated Chinese and 
      Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Yin, Kai
AU  - Yin K
AD  - College of Traditional Chinese Medicine, College of Integrated Chinese and 
      Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Xia, Jinyan
AU  - Xia J
AD  - College of Traditional Chinese Medicine, College of Integrated Chinese and 
      Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
FAU - Xue, Mei
AU  - Xue M
AD  - College of Traditional Chinese Medicine, College of Integrated Chinese and 
      Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20221024
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9638067
OTO - NOTNLM
OT  - Danggui Buxue decoction
OT  - gut microbiota
OT  - inflammation
OT  - isoflavone
OT  - metabolism
OT  - oxidative stress
OT  - traditional Chinese medicine
OT  - type 2 diabetes mellitus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/11/11 06:00
MHDA- 2022/11/11 06:01
PMCR- 2022/10/24
CRDT- 2022/11/10 02:18
PHST- 2022/08/27 00:00 [received]
PHST- 2022/09/26 00:00 [accepted]
PHST- 2022/11/10 02:18 [entrez]
PHST- 2022/11/11 06:00 [pubmed]
PHST- 2022/11/11 06:01 [medline]
PHST- 2022/10/24 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.1029409 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Oct 24;13:1029409. doi: 10.3389/fmicb.2022.1029409. 
      eCollection 2022.