PMID- 36353916
OWN - NLM
STAT- MEDLINE
DCOM- 20230118
LR  - 20230215
IS  - 1616-5195 (Electronic)
IS  - 1616-5187 (Linking)
VI  - 23
IP  - 1
DP  - 2023 Jan
TI  - Laponite-Gelatin Nanofibrous Microsphere Promoting Human Dental Follicle Stem 
      Cells Attachment and Osteogenic Differentiation for Noninvasive Stem Cell 
      Transplantation.
PG  - e2200347
LID - 10.1002/mabi.202200347 [doi]
AB  - Nanofibrous microspheres (NFM) are emerging as prominent next-generation 
      biomimetic injectable scaffold system for stem cell delivery and different tissue 
      regeneration where nanofibrous topography facilitates ECM-like stem cells niches. 
      Addition of osteogenic bioactive nanosilicate platelets within NFM can provide 
      osteoconductive cues to facilitate matrix mediated osteogenic differentiation of 
      stem cells and enhance the efficiency of bone tissue regeneration. In this study, 
      gelatin nanofibrous microspheres are prepared containing fluoride-doped laponite 
      XL21 (LP) using the emulsion mediated thermal induce phase separation (TIPS) 
      technique. Systematic studies are performed to understand the effect of 
      physicochemical properties of biomimicking NFM alone and with different 
      concentrations of LP on human dental follicle stem cells (hDFSCs), their cellular 
      attachment, proliferation, and osteogenic differentiation. The study highlights 
      the effect of LP nanosilicate with biomimicking nanofibrous injectable scaffold 
      system aiding in enhancing stem cell differentiation under normal physiological 
      conditions compared to NFM without LP. The laponite-NFM shows suitability as 
      excellent injectable biomaterials system for stem cell attachment, proliferation 
      and osteogenic differentiation for stem cell transplantation and bone tissue 
      regeneration.
CI  - (c) 2022 Wiley-VCH GmbH.
FAU - Sarviya, Nandini
AU  - Sarviya N
AUID- ORCID: 0000-0003-1866-7228
AD  - Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, 
      Telangana, 502285, India.
AD  - Department of Chemistry and Biotechnology, Swinburne Institute of Technology, 
      Hawthorn, Victoria, Vic-3122, Australia.
FAU - Basu, Suparna Mercy
AU  - Basu SM
AUID- ORCID: 0000-0001-7525-9090
AD  - Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, 
      Telangana, 502285, India.
FAU - Induvahi, Veernala
AU  - Induvahi V
AUID- ORCID: 0000-0002-4066-2127
AD  - Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, 
      Telangana, 502285, India.
FAU - Giri, Jyotsnendu
AU  - Giri J
AUID- ORCID: 0000-0002-2313-4912
AD  - Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, 
      Telangana, 502285, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221117
PL  - Germany
TA  - Macromol Biosci
JT  - Macromolecular bioscience
JID - 101135941
RN  - 9000-70-8 (Gelatin)
RN  - D703131383 (laponite)
SB  - IM
MH  - Humans
MH  - *Osteogenesis
MH  - Gelatin/pharmacology/chemistry
MH  - Microspheres
MH  - *Nanofibers/chemistry
MH  - Dental Sac
MH  - Cell Differentiation
MH  - Stem Cell Transplantation
MH  - Tissue Scaffolds/chemistry
MH  - Tissue Engineering/methods
OTO - NOTNLM
OT  - biocompatible
OT  - gelatin
OT  - injectable stem cell carrier
OT  - laponite XL21
OT  - nanofibrous microsphere
OT  - osteogenic differentiation
EDAT- 2022/11/11 06:00
MHDA- 2023/01/19 06:00
CRDT- 2022/11/10 03:44
PHST- 2022/10/28 00:00 [revised]
PHST- 2022/08/19 00:00 [received]
PHST- 2022/11/11 06:00 [pubmed]
PHST- 2023/01/19 06:00 [medline]
PHST- 2022/11/10 03:44 [entrez]
AID - 10.1002/mabi.202200347 [doi]
PST - ppublish
SO  - Macromol Biosci. 2023 Jan;23(1):e2200347. doi: 10.1002/mabi.202200347. Epub 2022 
      Nov 17.