PMID- 36354688
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230308
IS  - 1467-3045 (Electronic)
IS  - 1467-3037 (Print)
IS  - 1467-3037 (Linking)
VI  - 44
IP  - 11
DP  - 2022 Nov 9
TI  - Altered Expression of Interleukin-18 System mRNA at the Level of Endometrial 
      Myometrial Interface in Women with Adenomyosis.
PG  - 5550-5561
LID - 10.3390/cimb44110376 [doi]
AB  - Adenomyosis is a uterine pathology characterized by a deep invasion of 
      endometrial glands and stroma, disrupting the endometrial-myometrial interface 
      (EMI). Interleukin-18 (IL-18) system is a dominant cytokine involved in the 
      menstrual cycle of human endometrium. IL-18 may play a defensive role against 
      maternal immune response in the uterine cavity. The present study was designed to 
      determine IL-18-mediated immune response at the level of EMI. We uncovered that 
      mRNA of IL-18 system, including IL-18, IL-18 receptor (IL-18R), and its 
      antagonist, IL-18 binding protein (IL-18BP), expressed in eutopic, ectopic 
      endometrium, and corresponding myometrium in patients with adenomyosis. IL-18 
      system was demonstrated in paired tissue samples by immunochemistry and 
      immunofluorescence study. According to RT-PCR with CT value quantification and 
      2-∆∆Ct method, a significant down-regulation of IL-18BP in corresponding 
      myometrium in comparison to eutopic endometrium (p < 0.05) indicates that the 
      IL-18 system acts as a local immune modulator at the level of EMI and regulating 
      cytokine networks in the pathogenesis of adenomyosis. Furthermore, an increased 
      IL-18 antagonist to agonist ratio was noted in ectopic endometrium compared with 
      corresponding myometrium. We suggest that altered IL-18 system expression 
      contributes to immunological dysfunction and junctional zone disturbance in women 
      with adenomyosis.
FAU - Chen, Liang-Hsuan
AU  - Chen LH
AUID- ORCID: 0000-0002-5749-6962
AD  - Department of Obstetrics and Gynecology, Linkou Medical Center, Chang Gung 
      Memorial Hospital, Taoyuan 33305, Taiwan.
AD  - Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung 
      University, Taoyuan 33302, Taiwan.
FAU - Chan, She-Hung
AU  - Chan SH
AD  - Department of Obstetrics and Gynecology, College of Medicine, Chang Gung 
      University, Taoyuan 33302, Taiwan.
AD  - Department of Cosmetic Science, Providence University, No. 200, Sec. 7, Taiwan 
      Boulevard, Shalu Dist., Taichung 43301, Taiwan.
FAU - Li, Chin-Jung
AU  - Li CJ
AD  - Department of Obstetrics and Gynecology, Linkou Medical Center, Chang Gung 
      Memorial Hospital, Taoyuan 33305, Taiwan.
FAU - Wu, Hsien-Ming
AU  - Wu HM
AD  - Department of Obstetrics and Gynecology, Linkou Medical Center, Chang Gung 
      Memorial Hospital, Taoyuan 33305, Taiwan.
AD  - Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung 
      University, Taoyuan 33302, Taiwan.
FAU - Huang, Hong-Yuan
AU  - Huang HY
AD  - Department of Obstetrics and Gynecology, Linkou Medical Center, Chang Gung 
      Memorial Hospital, Taoyuan 33305, Taiwan.
AD  - Department of Obstetrics and Gynecology, College of Medicine, Chang Gung 
      University, Taoyuan 33302, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20221109
PL  - Switzerland
TA  - Curr Issues Mol Biol
JT  - Current issues in molecular biology
JID - 100931761
PMC - PMC9689074
OTO - NOTNLM
OT  - PCR
OT  - adenomyosis
OT  - cytokine
OT  - endometrium
OT  - interleukin-18
COIS- The authors declare no conflict of interest.
EDAT- 2022/11/11 06:00
MHDA- 2022/11/11 06:01
PMCR- 2022/11/09
CRDT- 2022/11/10 09:43
PHST- 2022/09/24 00:00 [received]
PHST- 2022/11/05 00:00 [revised]
PHST- 2022/11/06 00:00 [accepted]
PHST- 2022/11/10 09:43 [entrez]
PHST- 2022/11/11 06:00 [pubmed]
PHST- 2022/11/11 06:01 [medline]
PHST- 2022/11/09 00:00 [pmc-release]
AID - cimb44110376 [pii]
AID - cimb-44-00376 [pii]
AID - 10.3390/cimb44110376 [doi]
PST - epublish
SO  - Curr Issues Mol Biol. 2022 Nov 9;44(11):5550-5561. doi: 10.3390/cimb44110376.