PMID- 36355111
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230308
IS  - 2218-1989 (Print)
IS  - 2218-1989 (Electronic)
IS  - 2218-1989 (Linking)
VI  - 12
IP  - 11
DP  - 2022 Oct 26
TI  - Attenuation of Palmitic Acid-Induced Intestinal Epithelial Barrier Dysfunction by 
      6-Shogaol in Caco-2 Cells: The Role of MiR-216a-5p/TLR4/NF-kappaB Axis.
LID - 10.3390/metabo12111028 [doi]
LID - 1028
AB  - Palmitic acid (PA) can lead to intestinal epithelial barrier dysfunction. In this 
      study, the protective effects and working mechanisms of 6-shogaol against 
      PA-induced intestinal barrier dysfunction were investigated in human intestinal 
      epithelial Caco-2 cells. Transepithelial electrical resistance (TEER), 
      paracellular flux, qRT-PCR, immunofluorescence, and Western blot experiments 
      showed that the 24-h treatment with 400 muM PA damaged intestinal barrier 
      integrity, as evidenced by a reduction of 48% in the TEER value, a 4.1-fold 
      increase in the flux of fluorescein isothiocyanate-dextran 4000 (FD-4), and 
      decreases in the mRNA and protein expression of tight junction (TJ)-associated 
      proteins (claudin-1, occludin, and ZO-1), compared with the control. The PA 
      treatment significantly (p < 0.05) increased the levels of pro-inflammatory 
      cytokines (interleukin (IL)-6, IL-1beta, and tumor necrosis factor-alpha (TNF-alpha)) in 
      Caco-2 cells due to the upregulation of toll-like receptor 4 (TLR4), myeloid 
      differentiation factor 88 (MyD88), phosphorylated nuclear factor kappa-B (NF-kappaB) 
      proteins, and downregulation of miR-216a-5p (which directly targeted TLR4). 
      Co-treatment with PA and 6-shogaol (2.5 muM) significantly (p < 0.05) attenuated 
      PA-induced changes through regulation of TJs via the miR-216a-5p/TLR4/NF-kappaB 
      signaling pathway. This study provides insights into the functions and working 
      mechanisms of 6-shogaol as a promising food-derived agent against PA-induced 
      intestinal epithelial barrier dysfunction.
FAU - Ouyang, Fangxin
AU  - Ouyang F
AD  - College of Food Science and Engineering, Shandong Agricultural University, 61 
      Daizong Street, Taian 271018, China.
FAU - Li, Bo
AU  - Li B
AD  - Department of Nursing, Jinan Vocational College of Nursing, 3636 Gangxi Road, 
      Jinan 250102, China.
FAU - Wang, Yuli
AU  - Wang Y
AD  - College of Food Science and Engineering, Shandong Agricultural University, 61 
      Daizong Street, Taian 271018, China.
FAU - Xu, Longhua
AU  - Xu L
AD  - College of Food Science and Engineering, Shandong Agricultural University, 61 
      Daizong Street, Taian 271018, China.
FAU - Li, Dapeng
AU  - Li D
AD  - College of Food Science and Engineering, Shandong Agricultural University, 61 
      Daizong Street, Taian 271018, China.
FAU - Li, Feng
AU  - Li F
AUID- ORCID: 0000-0003-0611-2735
AD  - College of Food Science and Engineering, Shandong Agricultural University, 61 
      Daizong Street, Taian 271018, China.
FAU - Sun-Waterhouse, Dongxiao
AU  - Sun-Waterhouse D
AD  - School of Chemical Sciences, The University of Auckland, Private Bag 92019, 
      Auckland 1142, New Zealand.
LA  - eng
GR  - 2021TZXD007/Project of Key R&amp;D Program of Shandong Province/
GR  - N/A/Incubation Program of Youth Innovation in Shandong Province/
GR  - ZR2022MC004/Natural Science Foundation of Shandong Province/
PT  - Journal Article
DEP - 20221026
PL  - Switzerland
TA  - Metabolites
JT  - Metabolites
JID - 101578790
PMC - PMC9692742
OTO - NOTNLM
OT  - 6-shogaol
OT  - intestinal epithelial barrier
OT  - miR-216a-5p/TLR4
OT  - palmitic acid
OT  - tight junctions
COIS- The authors declare no conflict of interest.
EDAT- 2022/11/11 06:00
MHDA- 2022/11/11 06:01
PMCR- 2022/10/26
CRDT- 2022/11/10 11:14
PHST- 2022/09/22 00:00 [received]
PHST- 2022/10/19 00:00 [revised]
PHST- 2022/10/21 00:00 [accepted]
PHST- 2022/11/10 11:14 [entrez]
PHST- 2022/11/11 06:00 [pubmed]
PHST- 2022/11/11 06:01 [medline]
PHST- 2022/10/26 00:00 [pmc-release]
AID - metabo12111028 [pii]
AID - metabolites-12-01028 [pii]
AID - 10.3390/metabo12111028 [doi]
PST - epublish
SO  - Metabolites. 2022 Oct 26;12(11):1028. doi: 10.3390/metabo12111028.