PMID- 36356014
OWN - NLM
STAT- MEDLINE
DCOM- 20221114
LR  - 20230125
IS  - 2072-6651 (Electronic)
IS  - 2072-6651 (Linking)
VI  - 14
IP  - 11
DP  - 2022 Nov 4
TI  - Determination of Zearalenone and Its Derivatives in Feed by Gas 
      Chromatography-Mass Spectrometry with Immunoaffinity Column Cleanup and Isotope 
      Dilution.
LID - 10.3390/toxins14110764 [doi]
LID - 764
AB  - In this study, a gas chromatography-mass spectrometry (GC-MS) method was 
      established for the determination of zearalenone and its five derivatives in 
      feed, including zearalanone, alpha-zearalanol, beta-zearalanol, alpha-zearalenol, and 
      beta-zearalenol. An effective immunoaffinity column was prepared for sample 
      purification, which was followed by the silane derivatization of the eluate after 
      an immunoaffinity chromatography analysis for target compounds by GC-MS. Matrix 
      effects were corrected by an isotope internal standard of zearalenone in this 
      method. The six analytes had a good linear relationship in the range of 2-500 
      ng/mL, and the correlation coefficients were all greater than 0.99. The limits of 
      detection (LODs) and limits of quantification (LOQs) were less than 1.5 mug/kg and 
      5.0 mug/kg, respectively. The average spike recoveries for the six feed matrices 
      ranged from 89.6% to 112.3% with relative standard deviations (RSDs) less than 
      12.6%. Twenty actual feed samples were analyzed using the established method, and 
      at least one target was detected. The established GC-MS method was proven to be 
      reliable and suitable for the determination of zearalenone and its derivatives in 
      feed.
FAU - Luo, Sunlin
AU  - Luo S
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China.
FAU - Guo, Qi
AU  - Guo Q
AD  - Clover Technology Group Inc., Beijing 100044, China.
FAU - Wang, Xiong
AU  - Wang X
AD  - Clover Technology Group Inc., Beijing 100044, China.
FAU - Tian, Ying
AU  - Tian Y
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China.
FAU - Yang, Wenjun
AU  - Yang W
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China.
FAU - Li, Juntao
AU  - Li J
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China.
FAU - Chen, Yiqiang
AU  - Chen Y
AD  - State Key Laboratory of Animal Nutrition, College of Animal Science and 
      Technology, China Agricultural University, Beijing 100193, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221104
PL  - Switzerland
TA  - Toxins (Basel)
JT  - Toxins
JID - 101530765
RN  - 5W827M159J (Zearalenone)
RN  - 0 (zearalenol)
RN  - 0 (Isotopes)
SB  - IM
MH  - *Zearalenone/analysis
MH  - Gas Chromatography-Mass Spectrometry
MH  - Chromatography, High Pressure Liquid/methods
MH  - Tandem Mass Spectrometry/methods
MH  - Isotopes
PMC - PMC9697342
OTO - NOTNLM
OT  - GC-MS
OT  - feed
OT  - immunoaffinity column
OT  - isotope dilution
OT  - zearalenone and its derivatives
COIS- The authors declare no conflict of interest.
EDAT- 2022/11/11 06:00
MHDA- 2022/11/15 06:00
PMCR- 2022/11/04
CRDT- 2022/11/10 13:54
PHST- 2022/09/11 00:00 [received]
PHST- 2022/10/02 00:00 [revised]
PHST- 2022/11/02 00:00 [accepted]
PHST- 2022/11/10 13:54 [entrez]
PHST- 2022/11/11 06:00 [pubmed]
PHST- 2022/11/15 06:00 [medline]
PHST- 2022/11/04 00:00 [pmc-release]
AID - toxins14110764 [pii]
AID - toxins-14-00764 [pii]
AID - 10.3390/toxins14110764 [doi]
PST - epublish
SO  - Toxins (Basel). 2022 Nov 4;14(11):764. doi: 10.3390/toxins14110764.