PMID- 36356060
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221127
IS  - 2306-7381 (Electronic)
IS  - 2306-7381 (Linking)
VI  - 9
IP  - 11
DP  - 2022 Oct 22
TI  - HER2 Overexpression and Cytogenetical Patterns in Canine Mammary Carcinomas.
LID - 10.3390/vetsci9110583 [doi]
LID - 583
AB  - Human epidermal growth factor receptor 2 (HER2) is a tyrosine kinase receptor 
      that promotes tumor cell growth and is implicated in the pathogenesis of human 
      breast cancer. The role of HER2 in canine mammary carcinomas (CMCs) is not clear. 
      Therefore, this study aimed to examine the protein expression and cytogenetic 
      changes of HER2 and their correlation with other clinical-pathological parameters 
      in CMC. We retrospectively selected 112 CMCs. HER2, ER, and Ki67 were assessed by 
      immunohistochemistry. HER2 antibody validation was investigated by immunoblot on 
      mammary tumor cell lines. Fluorescence in situ hybridization (FISH) was performed 
      with probes for HER2 and CRYBA1 (control gene present on CFA9). HER2 protein 
      overexpression was detected in 15 carcinomas (13.5%). A total of 90 carcinomas 
      were considered technically adequate by FISH, and 8 out of 90 CMC (10%) were HER2 
      amplified, 3 of which showed a cluster-type pattern. HER2 overexpression was 
      correlated with an increased number of HER2 gene copies (p = 0.01; R = 0.24) and 
      overall survival (p = 0.03), but no correlation with ER, Ki67, grade, metastases, 
      and tumor-specific survival was found. Surprisingly, co-amplification or polysomy 
      was identified in three tumors, characterized by an increased copy number of both 
      HER2 and CRYBA1. A morphological translocation-fusion pattern was recognized in 
      20 carcinomas (22%), with a co-localized signal of HER2 and CRYBA1. HER2 is not 
      associated with clinical-pathological parameters of increased malignancy in 
      canine mammary tumors, but it is suitable for studying different amplification 
      patterns.
FAU - Muscatello, L V
AU  - Muscatello LV
AUID- ORCID: 0000-0003-4653-0624
AD  - Department of Veterinary Medical Sciences, University of Bologna, Ozzano 
      dell'Emilia, 40064 Bologna, Italy.
FAU - Gobbo, F
AU  - Gobbo F
AUID- ORCID: 0000-0002-4082-4675
AD  - Department of Veterinary Medical Sciences, University of Bologna, Ozzano 
      dell'Emilia, 40064 Bologna, Italy.
FAU - Di Oto, E
AU  - Di Oto E
AD  - OACP IE Ltd., T12H1XY Cork, Ireland.
FAU - Sarli, G
AU  - Sarli G
AUID- ORCID: 0000-0001-6405-3484
AD  - Department of Veterinary Medical Sciences, University of Bologna, Ozzano 
      dell'Emilia, 40064 Bologna, Italy.
FAU - De Maria, R
AU  - De Maria R
AD  - Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, Italy.
FAU - De Leo, A
AU  - De Leo A
AUID- ORCID: 0000-0002-3761-5135
AD  - Department of Experimental, Diagnostic and Specialty Medicine, University of 
      Bologna, 40138 Bologna, Italy.
AD  - Solid Tumor Molecular Pathology Laboratory, IRCCS Azienda 
      Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy.
FAU - Tallini, G
AU  - Tallini G
AUID- ORCID: 0000-0003-0113-6682
AD  - Department of Experimental, Diagnostic and Specialty Medicine, University of 
      Bologna, 40138 Bologna, Italy.
AD  - Solid Tumor Molecular Pathology Laboratory, IRCCS Azienda 
      Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy.
FAU - Brunetti, B
AU  - Brunetti B
AUID- ORCID: 0000-0002-2694-905X
AD  - Department of Veterinary Medical Sciences, University of Bologna, Ozzano 
      dell'Emilia, 40064 Bologna, Italy.
LA  - eng
GR  - RC-2022-2773377/Italian Ministry of Health/
PT  - Journal Article
DEP - 20221022
PL  - Switzerland
TA  - Vet Sci
JT  - Veterinary sciences
JID - 101680127
PMC - PMC9694975
OTO - NOTNLM
OT  - HER2 gene amplification
OT  - Ki67
OT  - canine mammary carcinoma
OT  - estrogen receptor
OT  - fluorescence in situ hybridization
OT  - immunohistochemistry
OT  - tissue microarray
COIS- The authors declare no conflicts of interest.
EDAT- 2022/11/11 06:00
MHDA- 2022/11/11 06:01
PMCR- 2022/10/22
CRDT- 2022/11/10 14:05
PHST- 2022/08/25 00:00 [received]
PHST- 2022/10/14 00:00 [revised]
PHST- 2022/10/20 00:00 [accepted]
PHST- 2022/11/10 14:05 [entrez]
PHST- 2022/11/11 06:00 [pubmed]
PHST- 2022/11/11 06:01 [medline]
PHST- 2022/10/22 00:00 [pmc-release]
AID - vetsci9110583 [pii]
AID - vetsci-09-00583 [pii]
AID - 10.3390/vetsci9110583 [doi]
PST - epublish
SO  - Vet Sci. 2022 Oct 22;9(11):583. doi: 10.3390/vetsci9110583.