PMID- 36358333
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221126
IS  - 2079-7737 (Print)
IS  - 2079-7737 (Electronic)
IS  - 2079-7737 (Linking)
VI  - 11
IP  - 11
DP  - 2022 Nov 8
TI  - Molecular Characterization of Secreted Factors and Extracellular 
      Vesicles-Embedded miRNAs from Bone Marrow-Derived Mesenchymal Stromal Cells in 
      Presence of Synovial Fluid from Osteoarthritis Patients.
LID - 10.3390/biology11111632 [doi]
LID - 1632
AB  - Bone marrow-derived mesenchymal stromal cells (BMSCs)-based therapies show a 
      great potential to manage inflammation and tissue degeneration in osteoarthritis 
      (OA) patients. Clinical trials showed the ability to manage pain and activation 
      of immune cells and allowed restoration of damaged cartilage. To date, a 
      molecular fingerprint of BMSC-secreted molecules in OA joint conditions able to 
      support clinical outcomes is missing; the lack of that molecular bridge between 
      BMSC activity and clinical results hampers clinical awareness and translation 
      into practice. In this study, BMSCs were cultured in synovial fluid (SF) obtained 
      from OA patients and, for the first time, a thorough characterization of soluble 
      factors and extracellular vesicles (EVs)-embedded miRNAs was performed in this 
      condition. Molecular data were sifted through the sieve of molecules and pathways 
      characterizing the OA phenotype in immune cells and joint tissues. One-hundred 
      and twenty-five secreted factors and one-hundred and ninety-two miRNAs were 
      identified. The combined action of both types of molecules was shown to, first, 
      foster BMSCs interaction with the most important OA immune cells, such as 
      macrophages and T cells, driving their switch towards an anti-inflammatory 
      phenotype and, second, promote cartilage homeostasis assisting chondrocyte 
      proliferation and attenuating the imbalance between destructive and protective 
      extracellular matrix-related players. Overall, molecular data give an 
      understanding of the clinical results observed in OA patients and can enable a 
      faster translation of BMSC-based products into everyday clinical practice.
FAU - Ragni, Enrico
AU  - Ragni E
AUID- ORCID: 0000-0003-0272-7417
AD  - Laboratorio di Biotecnologie applicate all'Ortopedia, IRCCS Istituto Ortopedico 
      Galeazzi, Via R. Galeazzi 4, I-20161 Milan, Italy.
FAU - Perucca Orfei, Carlotta
AU  - Perucca Orfei C
AUID- ORCID: 0000-0001-6162-8668
AD  - Laboratorio di Biotecnologie applicate all'Ortopedia, IRCCS Istituto Ortopedico 
      Galeazzi, Via R. Galeazzi 4, I-20161 Milan, Italy.
FAU - Valli, Federico
AU  - Valli F
AD  - Chirurgia Articolare Sostitutiva e Chirurgia Ortopedica (CASCO), IRCCS Istituto 
      Ortopedico Galeazzi, Via R. Galeazzi 4, I-20161 Milan, Italy.
FAU - Zagra, Luigi
AU  - Zagra L
AD  - Hip Department, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi 4, I-20161 
      Milan, Italy.
FAU - de Girolamo, Laura
AU  - de Girolamo L
AUID- ORCID: 0000-0002-9979-3092
AD  - Laboratorio di Biotecnologie applicate all'Ortopedia, IRCCS Istituto Ortopedico 
      Galeazzi, Via R. Galeazzi 4, I-20161 Milan, Italy.
LA  - eng
GR  - RCR-2021-23671217/Ministero della Salute/
GR  - Ricerca Corrente/Ministero della Salute/
PT  - Journal Article
DEP - 20221108
PL  - Switzerland
TA  - Biology (Basel)
JT  - Biology
JID - 101587988
PMC - PMC9687557
OTO - NOTNLM
OT  - cartilage
OT  - extracellular vesicles
OT  - immune cells
OT  - mesenchymal stromal cells
OT  - miRNAs
OT  - osteoarthritis
OT  - secretome
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2022/11/12 06:00
MHDA- 2022/11/12 06:01
PMCR- 2022/11/08
CRDT- 2022/11/11 01:04
PHST- 2022/10/03 00:00 [received]
PHST- 2022/11/02 00:00 [revised]
PHST- 2022/11/04 00:00 [accepted]
PHST- 2022/11/11 01:04 [entrez]
PHST- 2022/11/12 06:00 [pubmed]
PHST- 2022/11/12 06:01 [medline]
PHST- 2022/11/08 00:00 [pmc-release]
AID - biology11111632 [pii]
AID - biology-11-01632 [pii]
AID - 10.3390/biology11111632 [doi]
PST - epublish
SO  - Biology (Basel). 2022 Nov 8;11(11):1632. doi: 10.3390/biology11111632.