PMID- 36359292
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230308
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 10
IP  - 11
DP  - 2022 Nov 1
TI  - Chondrogenic Potential of Human Umbilical Cord Mesenchymal Stem Cells Cultured 
      with Exosome-Depleted Fetal Bovine Serum in an Osteoarthritis Mouse Model.
LID - 10.3390/biomedicines10112773 [doi]
LID - 2773
AB  - Osteoarthritis (OA) is characterized by the loss of articular cartilage and is 
      also an age-related disease. Recently, stem cell therapy for cartilage repair has 
      emerged. The stem cells need to be cultured with a fetal bovine serum 
      (FBS)-supplemented medium. The effect of FBS-containing exosomes on the 
      differentiation of human umbilical cord mesenchymal stem cells (HUCMSCs) is 
      unknown. The morphology, proliferation, surface marker expressions, and 
      trilineage differentiation ability of two groups of HUCMSCs, cultured with 
      conventional (FBS) and exosome-depleted FBS (Exo(-)FBS), were evaluated. In a 
      mouse OA model after two groups of HUCMSCs transplantation, the rotarod activity, 
      histology, and immunohistochemistry (type II collagen, aggrecan, IL-1beta, and 
      MMP13) of the cartilage were evaluated. The Exo(-)FBS-cultured HUCMSCs, like 
      FBS-cultured HUCMSCs, displayed classic MSC characteristics, including 
      spindle-shaped morphology, surface marker expression (positive for CD44, CD73, 
      CD90, CD105, and HLA-ABC and negative for CD34, CD45, and HLA-DR), and trilineage 
      differentiation (chondrogenesis, osteogenesis, and adipogenesis). The 
      Exo(-)FBS-cultured HUCMSCs proliferated significantly slower than those of the 
      FBS-cultured HUCMSCs (p < 0.01). The trilineage gene expression of PPAR-gamma, FABP4, 
      APAL, type II collagen, aggrecan, and SOX9 was significantly increased in the 
      Exo(-)FBS-cultured HUCMSCs than in the FBS-cultured HUCMSCs and undifferentiated 
      controls. The Exo(-)FBS- and FBS-cultured HUCMSCs-transplanted mice showed a 
      better rotarod activity than in the control OA mice (n = 3 in each group). A 
      significant histological improvement in hyaline cartilage destruction after the 
      transplantation of both types of FBS-cultured HUCMSCs was noted when compared 
      with the OA knees. The Exo(-)FBS-cultured HUCMSCs-transplanted knees showed a 
      higher International Cartilage Repair Society histological score (p < 0.05), 
      staining intensity of type II collagen (p < 0.01), and aggrecan (p < 0.01) than 
      in the control knees. Moreover, both types of the FBS-cultured 
      HUCMSCs-transplanted knees significantly decreased the expression of MMP13 and 
      IL-1beta compared to that in the OA knees (p < 0.01). The Exo(-)FBS-cultured HUCMSCs 
      harbor chondrogenic potential and attenuated cartilage destruction in a mouse OA 
      model. Our study provides a basis for future clinical trials using 
      Exo(-)FBS-cultured stem cells to treat OA.
FAU - Chang, Yu-Hsun
AU  - Chang YH
AD  - Department of Pediatrics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Tzu Chi University, Hualien 970, Taiwan.
FAU - Wu, Kun-Chi
AU  - Wu KC
AD  - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Tzu Chi University, Hualien 970, Taiwan.
FAU - Ding, Dah-Ching
AU  - Ding DC
AUID- ORCID: 0000-0001-5105-068X
AD  - Department of Obstetrics and Gynecology, Hualien Tzu Chi Hospital, Buddhist Tzu 
      Chi Medical Foundation, Tzu Chi University, Hualien 970, Taiwan.
AD  - Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan.
LA  - eng
GR  - TCRD 108-57, 108-49, 109-62, TCRD 111-071/Hualien Tzu Chi Medical Center/
GR  - TCMF-EP 111-01, TCMF-CP 111-05/Buddhist Tzu Chi Medical Foundation/
PT  - Journal Article
DEP - 20221101
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC9687487
OTO - NOTNLM
OT  - cartilage
OT  - exosome
OT  - mesenchymal stem cells
OT  - osteoarthritis
OT  - regeneration
OT  - umbilical cord
COIS- The authors declare no conflict of interest.
EDAT- 2022/11/12 06:00
MHDA- 2022/11/12 06:01
PMCR- 2022/11/01
CRDT- 2022/11/11 01:10
PHST- 2022/09/16 00:00 [received]
PHST- 2022/10/19 00:00 [revised]
PHST- 2022/10/27 00:00 [accepted]
PHST- 2022/11/11 01:10 [entrez]
PHST- 2022/11/12 06:00 [pubmed]
PHST- 2022/11/12 06:01 [medline]
PHST- 2022/11/01 00:00 [pmc-release]
AID - biomedicines10112773 [pii]
AID - biomedicines-10-02773 [pii]
AID - 10.3390/biomedicines10112773 [doi]
PST - epublish
SO  - Biomedicines. 2022 Nov 1;10(11):2773. doi: 10.3390/biomedicines10112773.