PMID- 36359394 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20221126 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 10 IP - 11 DP - 2022 Nov 9 TI - Lactobacillus Strains for Vegetable Juice Fermentation-Quality and Health Aspects. LID - 10.3390/biomedicines10112867 [doi] LID - 2867 AB - Vegetable juices are new carrier variants for beneficial bacteria, representing an alternative to dairy-fermented products, especially for vegan, strict vegetarian, or allergic consumers. The aim of this study was to characterize several Romanian native lactic acid bacteria (LAB) strains to select valuable nutritional and probiotic strains for vegetable juice fermentation. Nineteen LAB strains were analyzed for antibiotic susceptibility (disc-diffusion method), the presence of antibiotic resistance genes, the presence of functional genes. and the production of organic acids by HPLC. Antibiotic resistant strains were observed only with ampicillin (Amp10) and kanamycin (K30), 79% and 32%, respectively, with results partially confirmed by molecular analysis. Multiplex PCR revealed the presence of LBA1272, dltD, folP, agl, alpha-amy, malL, and ribA genes, related to stress resistance, starch metabolism, and production of vitamins, except for folK. HPLC analyses were performed on beet roots (SF), tomato (TM), and a mixture of carrots, celery, and beet (MTS) juices. High values of lactic acid were recorded in all cases of LAB fermentation (5034-14,176 microg/mL). The maximum values recorded for acetic acid did not exceed 2.5 mg/mL having a positive influence on the product's taste. FAU - Voaides, Catalina AU - Voaides C AUID- ORCID: 0000-0001-9351-3857 AD - Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania. FAU - Boiu-Sicuia, Oana AU - Boiu-Sicuia O AD - Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania. FAU - Israel-Roming, Florentina AU - Israel-Roming F AD - Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania. FAU - Zamfir, Medana AU - Zamfir M AD - Department of Microbiology, Institute of Biology Bucharest of the Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, Romania. FAU - Grosu-Tudor, Silvia Simona AU - Grosu-Tudor SS AD - Department of Microbiology, Institute of Biology Bucharest of the Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, Romania. FAU - Angelescu, Iulia Roxana AU - Angelescu IR AD - Department of Microbiology, Institute of Biology Bucharest of the Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, Romania. FAU - Cornea, Calina Petruta AU - Cornea CP AD - Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd., 011464 Bucharest, Romania. LA - eng GR - PN-III-P2-2.1-PED-2019-1675/Unitatea Executiva Pentru Finantarea Invatamantului Superior a Cercetarii Dezvoltarii si Inovarii/ PT - Journal Article DEP - 20221109 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC9687318 OTO - NOTNLM OT - fermented vegetable juices OT - functional beverages OT - lactic acid bacteria COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2022/11/12 06:00 MHDA- 2022/11/12 06:01 PMCR- 2022/11/09 CRDT- 2022/11/11 01:10 PHST- 2022/09/04 00:00 [received] PHST- 2022/11/05 00:00 [revised] PHST- 2022/11/07 00:00 [accepted] PHST- 2022/11/11 01:10 [entrez] PHST- 2022/11/12 06:00 [pubmed] PHST- 2022/11/12 06:01 [medline] PHST- 2022/11/09 00:00 [pmc-release] AID - biomedicines10112867 [pii] AID - biomedicines-10-02867 [pii] AID - 10.3390/biomedicines10112867 [doi] PST - epublish SO - Biomedicines. 2022 Nov 9;10(11):2867. doi: 10.3390/biomedicines10112867.