PMID- 36361778 OWN - NLM STAT- MEDLINE DCOM- 20221114 LR - 20221117 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 23 IP - 21 DP - 2022 Oct 26 TI - Identification of Key Genes during Ethylene-Induced Adventitious Root Development in Cucumber (Cucumis sativus L.). LID - 10.3390/ijms232112981 [doi] LID - 12981 AB - Ethylene (ETH), as a key plant hormone, plays critical roles in various processes of plant growth and development. ETH has been reported to induce adventitious rooting. Moreover, our previous studies have shown that exogenous ETH may induce plant adventitious root development in cucumber (Cucumis sativus L.). However, the key genes involved in this process are still unclear. To explore the key genes in ETH-induced adventitious root development, we employed a transcriptome technique and revealed 1415 differentially expressed genes (DEGs), with 687 DEGs up-regulated and 728 DEGs down-regulated. Using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, we further identified critical pathways that were involved in ETH-induced adventitious root development, including carbon metabolism (starch and sucrose metabolism, glycolysis/gluconeogenesis, citrate cycle (TCA cycle), oxidative phosphorylation, fatty acid biosynthesis, and fatty acid degradation), secondary metabolism (phenylalanine metabolism and flavonoid biosynthesis) and plant hormone signal transduction. In carbon metabolism, ETH reduced the content of sucrose, glucose, starch, the activity of sucrose synthase (SS), sucrose-phosphate synthase (SPS) and hexokinase (HK), and the expressions of CsHK2, pyruvate kinase2 (CsPK2), and CsCYP86A1, whereas it enhanced the expressions of beta-amylase 1 (CsBAM1) and beta-amylase 3 (CsBAM3). In secondary metabolism, the transcript levels of phenylalanine ammonia-lyase (CsPAL) and flavonoid 3'-monooxygenase (CsF3'M) were negatively regulated, and that of primary-amine oxidase (CsPAO) was positively regulated by ETH. Additionally, the indole-3-acetic acid (IAA) content and the expressions of auxin and ETH signaling transduction-related genes (auxin transporter-like protein 5 (CsLAX5), CsGH3.17, CsSUAR50, and CsERS) were suppressed, whereas the abscisic acid (ABA) content and the expressions of ABA and BR signaling transduction-related genes (CsPYL1, CsPYL5, CsPYL8, BRI1-associated kinase 1 (CsBAK1), and CsXTH3) were promoted by ETH. Furthermore, the mRNA levels of these genes were confirmed by real-time PCR (RT-qPCR). These results indicate that genes related to carbon metabolism, secondary metabolite biosynthesis, and plant hormone signaling transduction are involved in ETH-induced adventitious root development. This work identified the key pathways and genes in ETH-induced adventitious rooting in cucumber, which may provide new insights into ETH-induced adventitious root development and will be useful for investigating the molecular roles of key genes in this process in further studies. FAU - Deng, Yuzheng AU - Deng Y AD - College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China. FAU - Wang, Chunlei AU - Wang C AD - College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China. FAU - Zhang, Meiling AU - Zhang M AUID- ORCID: 0000-0001-8368-0387 AD - College of Science, Gansu Agricultural University, Lanzhou 730070, China. FAU - Wei, Lijuan AU - Wei L AD - College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China. FAU - Liao, Weibiao AU - Liao W AUID- ORCID: 0000-0002-7404-2145 AD - College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China. LA - eng GR - 2018YFD1000800/National Key Research and Development Program/ GR - 32072559/National Natural Science Foundation of China/ GR - 31860568/National Natural Science Foundation of China/ GR - 31560563/National Natural Science Foundation of China/ GR - 31160398/National Natural Science Foundation of China/ GR - 21YF5WA096/Key Research and Development Program of Gansu Province, China/ GR - 2018C-14/Research Fund of Higher Education of Gansu, China/ GR - 2019B-082/Research Fund of Higher Education of Gansu, China/ GR - 1606RJZA073/Natural Science Foundation of Gansu Province, China/ GR - 1606RJZA077/Natural Science Foundation of Gansu Province, China/ GR - 1606RJYA252/Natural Science Foundation of Gansu Province, China/ PT - Journal Article DEP - 20221026 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Plant Growth Regulators) RN - EC 3.2.1.2 (beta-Amylase) RN - 0 (Indoleacetic Acids) RN - 91GW059KN7 (ethylene) RN - 0 (Ethylenes) RN - 72S9A8J5GW (Abscisic Acid) RN - 9005-25-8 (Starch) RN - 57-50-1 (Sucrose) RN - 7440-44-0 (Carbon) RN - 0 (Fatty Acids) SB - IM MH - *Cucumis sativus/metabolism MH - Plant Growth Regulators/metabolism MH - *beta-Amylase/genetics/metabolism MH - Plant Roots/metabolism MH - Indoleacetic Acids/metabolism MH - Ethylenes/metabolism MH - Abscisic Acid/metabolism MH - Starch/metabolism MH - Sucrose/metabolism MH - Carbon/metabolism MH - Fatty Acids/metabolism MH - Gene Expression Regulation, Plant PMC - PMC9658848 OTO - NOTNLM OT - carbon metabolism OT - plant hormone signal transduction OT - secondary metabolism OT - transcriptome COIS- The authors declare no conflict of interest. EDAT- 2022/11/12 06:00 MHDA- 2022/11/15 06:00 PMCR- 2022/10/26 CRDT- 2022/11/11 01:25 PHST- 2022/09/28 00:00 [received] PHST- 2022/10/20 00:00 [revised] PHST- 2022/10/24 00:00 [accepted] PHST- 2022/11/11 01:25 [entrez] PHST- 2022/11/12 06:00 [pubmed] PHST- 2022/11/15 06:00 [medline] PHST- 2022/10/26 00:00 [pmc-release] AID - ijms232112981 [pii] AID - ijms-23-12981 [pii] AID - 10.3390/ijms232112981 [doi] PST - epublish SO - Int J Mol Sci. 2022 Oct 26;23(21):12981. doi: 10.3390/ijms232112981.