PMID- 36364921 OWN - NLM STAT- MEDLINE DCOM- 20221121 LR - 20221121 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 14 IP - 21 DP - 2022 Nov 3 TI - Antioxidant Capacity and Protective Effect of Cow Placenta Extract on D-Galactose-Induced Skin Aging in Mice. LID - 10.3390/nu14214659 [doi] LID - 4659 AB - Placental extract has been used for skin care and delaying skin aging. Cow placenta is an abundant resource with a large mass, which has not been harnessed effectively. Cow placenta extract (CPE) has the functions of antioxidation, anti-inflammatory, promoting growth and development, and promoting hair growth. However, little is known about the effect of oral administration of cow placenta extract on skin conditions. Therefore, the present study aimed to investigate the antioxidant capacity of CPE in vitro and in vivo and its protective effect on d-galactose (D-gal) induced skin aging in mice. The results showed that CPE had strong free radical scavenging, reducing and metal chelating activities. CPE can increase the activity of catalase (CAT), glutathione peroxidase (GSH-Px), peroxidase (POD), superoxide dismutase (SOD), and the content of glutathione (GSH), decrease the content of malondialdehyde (MDA). Moreover, CPE can decrease the gene and protein expression of matrix metalloproteinase 1a (MMP-1a) and matrix metalloproteinase 3 (MMP-3) and increase the expression of transforming growth factor-beta (TGF-beta) and tissue inhibitor of metalloproteinase 1 (TIMP-1) of mouse skin. Histopathological analysis showed CPE reduced the collagen damage caused by D-gal, increased collagen synthesis and reduced its degradation to delay skin aging. FAU - Shen, Liu-Hong AU - Shen LH AUID- ORCID: 0000-0002-2832-5806 AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. FAU - Fan, Lei AU - Fan L AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. FAU - Zhang, Yue AU - Zhang Y AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. FAU - Shen, Yu AU - Shen Y AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. FAU - Su, Zhe-Tong AU - Su ZT AD - College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China. FAU - Peng, Guang-Neng AU - Peng GN AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. FAU - Deng, Jun-Liang AU - Deng JL AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. FAU - Zhong, Zhi-Jun AU - Zhong ZJ AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. FAU - Wu, Xiao-Feng AU - Wu XF AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. FAU - Yu, Shu-Min AU - Yu SM AUID- ORCID: 0000-0002-8784-6003 AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. FAU - Cao, Sui-Zhong AU - Cao SZ AUID- ORCID: 0000-0003-0741-572X AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. FAU - Zong, Xiao-Lan AU - Zong XL AD - The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. LA - eng GR - 2019YJ0650/Sichuan Department of Science and Technology Support Project/ GR - 2221998011/Construction of Sichuan Agricultural University/ PT - Journal Article DEP - 20221103 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Antioxidants) RN - X2RN3Q8DNE (Galactose) RN - GAN16C9B8O (Glutathione) RN - EC 1.11.1.9 (Glutathione Peroxidase) RN - 4Y8F71G49Q (Malondialdehyde) RN - 0 (Plant Extracts) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - 0 (Tissue Inhibitor of Metalloproteinase-1) SB - IM MH - Animals MH - Cattle MH - Female MH - Mice MH - Pregnancy MH - *Antioxidants/pharmacology/metabolism MH - Galactose/metabolism MH - Glutathione/metabolism MH - Glutathione Peroxidase/metabolism MH - Malondialdehyde/metabolism MH - Oxidative Stress MH - Placenta/metabolism MH - Plant Extracts/pharmacology MH - *Skin Aging MH - Superoxide Dismutase/metabolism MH - Tissue Inhibitor of Metalloproteinase-1/metabolism PMC - PMC9654611 OTO - NOTNLM OT - D-galactose OT - antioxidant OT - cow OT - placenta OT - skin aging COIS- The authors declare no conflict of interest. EDAT- 2022/11/12 06:00 MHDA- 2022/11/15 06:00 PMCR- 2022/11/03 CRDT- 2022/11/11 01:45 PHST- 2022/10/11 00:00 [received] PHST- 2022/10/23 00:00 [revised] PHST- 2022/11/01 00:00 [accepted] PHST- 2022/11/11 01:45 [entrez] PHST- 2022/11/12 06:00 [pubmed] PHST- 2022/11/15 06:00 [medline] PHST- 2022/11/03 00:00 [pmc-release] AID - nu14214659 [pii] AID - nutrients-14-04659 [pii] AID - 10.3390/nu14214659 [doi] PST - epublish SO - Nutrients. 2022 Nov 3;14(21):4659. doi: 10.3390/nu14214659.