PMID- 36366483
OWN - NLM
STAT- MEDLINE
DCOM- 20221114
LR  - 20230130
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 14
IP  - 11
DP  - 2022 Oct 28
TI  - Studying the Interactions of U24 from HHV-6 in Order to Further Elucidate Its 
      Potential Role in MS.
LID - 10.3390/v14112384 [doi]
LID - 2384
AB  - A number of studies have suggested that human herpesvirus 6A (HHV-6A) may play a 
      role in multiple sclerosis (MS). Three possible hypotheses have been 
      investigated: (1) U24 from HHV-6A (U24-6A) mimics myelin basic protein (MBP) 
      through analogous phosphorylation and interaction with Fyn-SH3; (2) U24-6A 
      affects endocytic recycling by binding human neural precursor cell (NPC) 
      expressed developmentally down-regulated protein 4-like WW3* domain 
      (hNedd4L-WW3*); and (3) MS patients who express Killer Cell Immunoglobulin Like 
      Receptor 2DL2 (KIR2DL2) on natural killer (NK) cells are more susceptible to 
      HHV-6 infection. In this contribution, we examined the validity of these 
      propositions by investigating the interactions of U24 from HHV-6B (U24-6B), a 
      variant less commonly linked to MS, with Fyn-SH3 and hNedd4L-WW3* using 
      heteronuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) 
      titrations and isothermal titration calorimetry (ITC). In addition, the 
      importance of phosphorylation and the specific role of U24 in NK cell activation 
      in MS patients were examined. Overall, the findings allowed us to shed light into 
      the models linking HHV-6 to MS and the involvement of U24.
FAU - Pi, Keng-Shuo
AU  - Pi KS
AUID- ORCID: 0000-0003-3778-8121
AD  - Department of Chemistry, University of British Columbia, 2036 Main Mall, 
      Vancouver, BC V6T 1Z1, Canada.
FAU - Bortolotti, Daria
AU  - Bortolotti D
AUID- ORCID: 0000-0003-4496-9561
AD  - Department of Chemical, Pharmaceutical and Agricultural Sciences, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Sang, Yurou
AU  - Sang Y
AD  - Department of Chemistry, University of British Columbia, 2036 Main Mall, 
      Vancouver, BC V6T 1Z1, Canada.
AD  - Faculty of Health Sciences, Simon Fraser University, 8888 University Drive, 
      Burnaby, BC V5A 1S6, Canada.
FAU - Schiuma, Giovanna
AU  - Schiuma G
AUID- ORCID: 0000-0003-4652-5550
AD  - Department of Chemical, Pharmaceutical and Agricultural Sciences, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Beltrami, Silvia
AU  - Beltrami S
AD  - Department of Chemical, Pharmaceutical and Agricultural Sciences, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Rizzo, Sabrina
AU  - Rizzo S
AD  - Department of Chemical, Pharmaceutical and Agricultural Sciences, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Bortoluzzi, Alessandra
AU  - Bortoluzzi A
AD  - Rheumatology Unit, Department of Medical Sciences, University of Ferrara and 
      Azienda Ospedaliero-Universitaria S. Anna, 44121 Ferrara, Italy.
FAU - Baldi, Eleonora
AU  - Baldi E
AD  - Department of Neuroscience and Rehabilitation, Division of Neurology, "Sant'Anna" 
      University-Hospital, 44121 Ferrara, Italy.
FAU - Creagh, A Louise
AU  - Creagh AL
AD  - Michael Smith Laboratories and Department of Chemical and Biological Engineering, 
      University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4, Canada.
FAU - Haynes, Charles A
AU  - Haynes CA
AD  - Michael Smith Laboratories and Department of Chemical and Biological Engineering, 
      University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4, Canada.
FAU - Rizzo, Roberta
AU  - Rizzo R
AUID- ORCID: 0000-0001-9507-9126
AD  - Department of Chemical, Pharmaceutical and Agricultural Sciences, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Straus, Suzana K
AU  - Straus SK
AUID- ORCID: 0000-0002-5357-8450
AD  - Department of Chemistry, University of British Columbia, 2036 Main Mall, 
      Vancouver, BC V6T 1Z1, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221028
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
SB  - IM
MH  - Humans
MH  - *Herpesvirus 6, Human/physiology
MH  - *Roseolovirus Infections
MH  - *Multiple Sclerosis
MH  - Phosphorylation
MH  - Nuclear Magnetic Resonance, Biomolecular
PMC - PMC9696605
OTO - NOTNLM
OT  - Fyn-SH3
OT  - Killer Cell Immunoglobulin Like Receptor 2DL2
OT  - Nedd4L
OT  - Roseolovirus
OT  - human herpes virus
OT  - multiple sclerosis
OT  - natural killer cells
OT  - phosphorylation
COIS- The authors declare no conflict of interest.
EDAT- 2022/11/12 06:00
MHDA- 2022/11/15 06:00
PMCR- 2022/10/28
CRDT- 2022/11/11 01:56
PHST- 2022/09/20 00:00 [received]
PHST- 2022/10/27 00:00 [revised]
PHST- 2022/10/27 00:00 [accepted]
PHST- 2022/11/11 01:56 [entrez]
PHST- 2022/11/12 06:00 [pubmed]
PHST- 2022/11/15 06:00 [medline]
PHST- 2022/10/28 00:00 [pmc-release]
AID - v14112384 [pii]
AID - viruses-14-02384 [pii]
AID - 10.3390/v14112384 [doi]
PST - epublish
SO  - Viruses. 2022 Oct 28;14(11):2384. doi: 10.3390/v14112384.