PMID- 36369053
OWN - NLM
STAT- MEDLINE
DCOM- 20230515
LR  - 20230522
IS  - 1879-3096 (Electronic)
IS  - 0167-7799 (Linking)
VI  - 41
IP  - 6
DP  - 2023 Jun
TI  - Imaging the unimaginable: leveraging signal generation of CRISPR-Cas for 
      sensitive genome imaging.
PG  - 769-784
LID - S0167-7799(22)00274-8 [pii]
LID - 10.1016/j.tibtech.2022.10.003 [doi]
AB  - Fluorescence in situ hybridization (FISH) is the gold standard for visualizing 
      genomic DNA in fixed cells and tissues, but it is incompatible with live-cell 
      imaging, and its combination with RNA imaging is challenging. Consequently, due 
      to its capacity to bind double-stranded DNA (dsDNA) and design flexibility, the 
      clustered regularly interspaced short palindromic repeats 
      (CRISPR)-CRISPR-associated protein 9 (CRISPR-Cas9) technology has sparked 
      enormous interest over the past decade. In this review, we describe various 
      nucleic acid (NA)- and protein-based (amplified) signal generation methods that 
      achieve imaging of repetitive and single-copy sequences, and even 
      single-nucleotide variants (SNVs), next to highly multiplexed as well as dynamic 
      imaging in live cells. With future progress in the field, the 
      CRISPR-(d)Cas9-based technology promises to break through as a next-generation 
      cell-imaging technique.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Van Tricht, Charlotte
AU  - Van Tricht C
AD  - Department of Biosystems, MeBioS-Biosensors Group, KU Leuven, 3001 Leuven, 
      Belgium; KU Leuven Institute for Single Cell Omics (LISCO), KU Leuven, 3000 
      Leuven, Belgium.
FAU - Voet, Thierry
AU  - Voet T
AD  - KU Leuven Institute for Single Cell Omics (LISCO), KU Leuven, 3000 Leuven, 
      Belgium; Department of Human Genetics, Laboratory of Reproductive Genomics, KU 
      Leuven, 3000 Leuven, Belgium.
FAU - Lammertyn, Jeroen
AU  - Lammertyn J
AD  - Department of Biosystems, MeBioS-Biosensors Group, KU Leuven, 3001 Leuven, 
      Belgium; KU Leuven Institute for Single Cell Omics (LISCO), KU Leuven, 3000 
      Leuven, Belgium. Electronic address: jeroen.lammertyn@kuleuven.be.
FAU - Spasic, Dragana
AU  - Spasic D
AD  - Department of Biosystems, MeBioS-Biosensors Group, KU Leuven, 3001 Leuven, 
      Belgium; KU Leuven Institute for Single Cell Omics (LISCO), KU Leuven, 3000 
      Leuven, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20221108
PL  - England
TA  - Trends Biotechnol
JT  - Trends in biotechnology
JID - 8310903
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *CRISPR-Cas Systems
MH  - In Situ Hybridization, Fluorescence
MH  - *Genome
MH  - DNA/genetics/metabolism
MH  - Diagnostic Imaging
OTO - NOTNLM
OT  - CRISPR-Cas9
OT  - fixed-cell imaging
OT  - genome imaging
OT  - live-cell imaging
OT  - signal amplification
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2022/11/12 06:00
MHDA- 2023/05/15 06:42
CRDT- 2022/11/11 23:40
PHST- 2022/06/26 00:00 [received]
PHST- 2022/09/29 00:00 [revised]
PHST- 2022/10/11 00:00 [accepted]
PHST- 2023/05/15 06:42 [medline]
PHST- 2022/11/12 06:00 [pubmed]
PHST- 2022/11/11 23:40 [entrez]
AID - S0167-7799(22)00274-8 [pii]
AID - 10.1016/j.tibtech.2022.10.003 [doi]
PST - ppublish
SO  - Trends Biotechnol. 2023 Jun;41(6):769-784. doi: 10.1016/j.tibtech.2022.10.003. 
      Epub 2022 Nov 8.