PMID- 36369089 OWN - NLM STAT- MEDLINE DCOM- 20221115 LR - 20221117 IS - 1477-7819 (Electronic) IS - 1477-7819 (Linking) VI - 20 IP - 1 DP - 2022 Nov 11 TI - Downregulation of zinc finger protein 71 in laryngeal squamous cell carcinoma tissues and its potential molecular mechanism and clinical significance: a study based on immunohistochemistry staining and data mining. PG - 359 LID - 10.1186/s12957-022-02823-8 [doi] LID - 359 AB - BACKGROUND: The molecular mechanism of laryngeal squamous cell carcinoma (LSCC) is not completely clear, which leads to poor prognosis and treatment difficulties for LSCC patients. To date, no study has reported the exact expression level of zinc finger protein 71 (ZNF71) and its molecular mechanism in LSCC. METHODS: In-house immunohistochemistry (IHC) staining (33 LSCC samples and 29 non-LSCC samples) was utilized in analyzing the protein expression level of ZNF71 in LSCC. Gene chips and high-throughput sequencing data collected from multiple public resources (313 LSCC samples and 192 non-LSCC samples) were utilized in analyzing the exact mRNA expression level of ZNF71 in LSCC. Single-cell RNA sequencing (scRNA-seq) data was used to explore the expression status of ZNF71 in different LSCC subpopulations. Enrichment analysis of ZNF71, its positively and differentially co-expressed genes (PDCEGs), and its downstream target genes was employed to detect the potential molecular mechanism of ZNF71 in LSCC. Moreover, we conducted correlation analysis between ZNF71 expression and immune infiltration. RESULTS: ZNF71 was downregulated at the protein level (area under the curve [AUC] = 0.93, p < 0.0001) and the mRNA level (AUC = 0.71, p = 0.023) in LSCC tissues. Patients with nodal metastasis had lower protein expression level of ZNF71 than patients without nodal metastasis (p < 0.05), and male LSCC patients had lower mRNA expression level of ZNF71 than female LSCC patients (p < 0.01). ZNF71 was absent in different LSCC subpopulations, including cancer cells, plasma cells, and tumor-infiltrated immune cells, based on scRNA-seq analysis. Enrichment analysis showed that ZNF71 and its PDCEGs may influence the progression of LSCC by regulating downstream target genes of ZNF71. These downstream target genes of ZNF71 were mainly enriched in tight junctions. Moreover, downregulation of ZNF71 may influence the development and even therapy of LSCC by reducing immune infiltration. CONCLUSION: Downregulation of ZNF71 may promote the progression of LSCC by reducing tight junctions and immune infiltration; this requires further study. CI - (c) 2022. The Author(s). FAU - Jiang, Fang-Cheng AU - Jiang FC AD - Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Luo, Jia-Yuan AU - Luo JY AD - Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Dang, Yi-Wu AU - Dang YW AD - Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Lu, Hui-Ping AU - Lu HP AD - Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Li, Dong-Ming AU - Li DM AD - Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Huang, Zhi-Guang AU - Huang ZG AD - Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Tang, Yu-Lu AU - Tang YL AD - Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Fang, Ye-Ying AU - Fang YY AD - Department of Radiology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Tang, Yu-Xing AU - Tang YX AD - Department of Radiology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Su, Ya-Si AU - Su YS AD - Department of Pathology, Liuzhou People's Hospital, 8 Wenchang RD, Liuzhou, Guangxi Zhuang Autonomous Region, 545006, People's Republic of China. FAU - Dai, Wen-Bin AU - Dai WB AD - Department of Pathology, Liuzhou People's Hospital, 8 Wenchang RD, Liuzhou, Guangxi Zhuang Autonomous Region, 545006, People's Republic of China. FAU - Pan, Shang-Ling AU - Pan SL AD - Department of Pathophysiology, School of Pre-clinical Medicine, Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Feng, Zhen-Bo AU - Feng ZB AD - Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - Chen, Gang AU - Chen G AD - Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. FAU - He, Juan AU - He J AD - Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong RD, Nanning, Guangxi Zhuang Autonomous Region, 530021, People's Republic of China. hejuan@stu.gxmu.edu.cn. LA - eng GR - 2202JFA02/Guangxi Medical University Teacher Teaching Ability Development Project/ GR - WLXSZX21118/Future Academic Star of Guangxi Medical University/ GR - 202110598040/Guangxi Medical University Undergraduate Innovation and Entrepreneurship Training Program/ GR - 2022/Guangxi Higher Education Undergraduate Teaching Reform Project/ GR - 2021B167/Guangxi Educational Science Planning Key Project/ PT - Journal Article DEP - 20221111 PL - England TA - World J Surg Oncol JT - World journal of surgical oncology JID - 101170544 RN - 0 (RNA, Messenger) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - Male MH - Female MH - Squamous Cell Carcinoma of Head and Neck/genetics MH - *Laryngeal Neoplasms/genetics/pathology MH - Down-Regulation MH - Immunohistochemistry MH - *Carcinoma, Squamous Cell/pathology MH - *Head and Neck Neoplasms MH - RNA, Messenger/genetics MH - Data Mining MH - Zinc Fingers MH - Staining and Labeling MH - Gene Expression Regulation, Neoplastic MH - Biomarkers, Tumor/genetics MH - Prognosis PMC - PMC9650879 OTO - NOTNLM OT - Immune infiltration OT - Immunohistochemistry (IHC) staining OT - Laryngeal squamous cell carcinoma (LSCC) OT - Molecular mechanism OT - Single-cell RNA sequencing (scRNA-seq) OT - Zinc finger protein 71 (ZNF71) COIS- The authors declare that they have no competing interests. EDAT- 2022/11/12 06:00 MHDA- 2022/11/16 06:00 PMCR- 2022/11/11 CRDT- 2022/11/11 23:43 PHST- 2022/06/17 00:00 [received] PHST- 2022/10/29 00:00 [accepted] PHST- 2022/11/11 23:43 [entrez] PHST- 2022/11/12 06:00 [pubmed] PHST- 2022/11/16 06:00 [medline] PHST- 2022/11/11 00:00 [pmc-release] AID - 10.1186/s12957-022-02823-8 [pii] AID - 2823 [pii] AID - 10.1186/s12957-022-02823-8 [doi] PST - epublish SO - World J Surg Oncol. 2022 Nov 11;20(1):359. doi: 10.1186/s12957-022-02823-8.