PMID- 36369946
OWN - NLM
STAT- MEDLINE
DCOM- 20221115
LR  - 20221222
IS  - 1949-0984 (Electronic)
IS  - 1949-0976 (Print)
IS  - 1949-0976 (Linking)
VI  - 14
IP  - 1
DP  - 2022 Jan-Dec
TI  - Kidney microbiota dysbiosis contributes to the development of hypertension.
PG  - 2143220
LID - 10.1080/19490976.2022.2143220 [doi]
LID - 2143220
AB  - Gut microbiota dysbiosis promotes metabolic syndromes (e.g., hypertension); 
      however, the patterns that drive hypertensive pathology and could be targeted for 
      therapeutic intervention are unclear. We hypothesized that gut microbes might 
      translocate to the kidney to trigger hypertension. We aimed to uncover their 
      method of colonization, and thereby how to maintain blood pressure homeostasis. 
      Using combined approaches based on fluorescence in situ hybridization (FISH) and 
      immunofluorescence staining, electron microscopy analysis, bacterial cultures, 
      species identification, and RNA-sequencing-based meta-transcriptomics, we first 
      demonstrated the presence of bacteria within the kidney of spontaneously 
      hypertensive rats (SHRs) and its normotensive counterpart, Wistar-Kyoto rats 
      (WKYs), and patients with hypertension. Translocated renal bacteria were coated 
      with secretory IgA (sIgA) or remained dormant in the L-form. Klebsiella 
      pneumoniae (K.pn) was identified in the kidneys of germ-free (GF) mice following 
      intestinal transplantation, which suggested an influx of gut bacteria into the 
      kidneys. Renal bacterial taxa and their function are associated with 
      hypertension. Hypertensive hosts showed increased richness in the pathobionts of 
      their kidneys, which were partly derived from the gastrointestinal tract. We also 
      demonstrated the indispensable role of bacterial IgA proteases in the 
      translocation of live microbes. Furthermore, Tartary buckwheat dietary 
      intervention reduced blood pressure and modulated the core renal flora-host 
      ecosystem to near-normal states. Taken together, the unique patterns of viable 
      and dormant bacteria in the kidney provide insight into the pathogenesis of 
      non-communicable chronic diseases and cardiometabolic diseases (e.g., 
      hypertension), and may lead to potential novel microbiota-targeted dietary 
      therapies.
FAU - Liu, Xin-Yu
AU  - Liu XY
AD  - State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University, Chengdu, China.
FAU - Li, Jing
AU  - Li J
AD  - Hypertension Center, State Key Laboratory of Cardiovascular Disease, National 
      Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical 
      Sciences and Peking Union Medical College, Beijing, China.
AD  - Heart Center & Beijing Key Laboratory of Hypertension, Beijing Chaoyang 
      Hospital, Capital Medical University, Beijing, China.
FAU - Zhang, Yamei
AU  - Zhang Y
AD  - Clinical Genetics Laboratory, Affiliated Hospital &Clinical Medical College 
      of Chengdu University, Chengdu, P.R. China.
AD  - School of Food and Biological Engineering, Chengdu University, Chengdu, China.
FAU - Fan, Luyun
AU  - Fan L
AD  - Hypertension Center, State Key Laboratory of Cardiovascular Disease, National 
      Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical 
      Sciences and Peking Union Medical College, Beijing, China.
FAU - Xia, Yanli
AU  - Xia Y
AD  - Clinical Genetics Laboratory, Affiliated Hospital &Clinical Medical College 
      of Chengdu University, Chengdu, P.R. China.
AD  - School of Food and Biological Engineering, Chengdu University, Chengdu, China.
FAU - Wu, Yongyang
AU  - Wu Y
AD  - Department of Urology, Affiliated Sanming First Hospital, Fujian Medical 
      University, Sanming, China.
FAU - Chen, Junru
AU  - Chen J
AD  - Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
FAU - Zhao, Xinyu
AU  - Zhao X
AD  - State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University, Chengdu, China.
FAU - Gao, Qiannan
AU  - Gao Q
AD  - Hypertension Center, State Key Laboratory of Cardiovascular Disease, National 
      Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical 
      Sciences and Peking Union Medical College, Beijing, China.
FAU - Xu, Bing
AU  - Xu B
AD  - Department of Proctology, University of Chinese Academy of Sciences-Shenzhen 
      Hospital (Guang Ming), Shenzhen, China.
FAU - Nie, Chunlai
AU  - Nie C
AD  - State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University, Chengdu, China.
FAU - Li, Zhengyu
AU  - Li Z
AD  - Department of Obstetrics and Gynecology, Key Laboratory of Birth Defects and 
      Related Diseases of Women and Children, Ministry of Education, West China Second 
      University Hospital, Sichuan University, Chengdu, China.
FAU - Tong, Aiping
AU  - Tong A
AD  - State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University, Chengdu, China.
FAU - Wang, Wenjie
AU  - Wang W
AD  - Hypertension Center, State Key Laboratory of Cardiovascular Disease, National 
      Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical 
      Sciences and Peking Union Medical College, Beijing, China.
FAU - Cai, Jun
AU  - Cai J
AD  - Hypertension Center, State Key Laboratory of Cardiovascular Disease, National 
      Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical 
      Sciences and Peking Union Medical College, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gut Microbes
JT  - Gut microbes
JID - 101495343
SB  - IM
MH  - Rats
MH  - Mice
MH  - Animals
MH  - Dysbiosis/microbiology
MH  - *Gastrointestinal Microbiome
MH  - In Situ Hybridization, Fluorescence
MH  - Rats, Inbred WKY
MH  - *Hypertension/etiology
MH  - *Microbiota/physiology
MH  - Kidney/metabolism
MH  - Bacteria/genetics
PMC - PMC9662196
OTO - NOTNLM
OT  - Gut microbiota translocation
OT  - IgA protease
OT  - L-form bacteria
OT  - bacterial translocation
OT  - cell-wall-deficient bacteria
OT  - dietary intervention
OT  - hypertension
OT  - kidney microbiota
OT  - sIgA-coated bacteria
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/11/13 06:00
MHDA- 2022/11/16 06:00
PMCR- 2022/11/12
CRDT- 2022/11/12 03:43
PHST- 2022/11/12 03:43 [entrez]
PHST- 2022/11/13 06:00 [pubmed]
PHST- 2022/11/16 06:00 [medline]
PHST- 2022/11/12 00:00 [pmc-release]
AID - 2143220 [pii]
AID - 10.1080/19490976.2022.2143220 [doi]
PST - ppublish
SO  - Gut Microbes. 2022 Jan-Dec;14(1):2143220. doi: 10.1080/19490976.2022.2143220.