PMID- 36370210
OWN - NLM
STAT- MEDLINE
DCOM- 20230106
LR  - 20230111
IS  - 1615-7605 (Electronic)
IS  - 1615-7591 (Linking)
VI  - 46
IP  - 1
DP  - 2023 Jan
TI  - Immobilization of lipase on silica nanoparticles by adsorption followed by 
      glutaraldehyde cross-linking.
PG  - 25-38
LID - 10.1007/s00449-022-02810-z [doi]
AB  - In this study, Candida antarctica lipase B was immobilized on silica (SiO(2)) 
      nanoparticles by physical adsorption, and then cross-linked with glutaraldehyde 
      (GA) to prepare cross-linked immobilized lipase (CLIL). During the condition of 
      1.28 mg/mL lipase concentration, 25 ℃ temperature, 2 h adsorption time, 0.01% GA 
      (V/V) 7.5 mL and 2 h cross-linking time, the highest recovery activity of CLIL 
      reached 87.82 +/- 0.07% (22.55 +/- 0.025 U/mg). Scanning electron microscope (SEM) 
      and confocal laser scanning microscope (CLSM) confirmed that lipase was 
      immobilized on the surface of SiO(2) nanoparticles. The changes in secondary 
      structures of CLIL indicated that cross-linking changed the secondary structure 
      of lipase protein, which made the structure of CLIL more stable. Compared with 
      the free lipase, the thermal stability and storage stability of CLIL was 
      significantly improved, and the t(1/2) at 60  degrees C was extended. Studies had shown 
      that it was a feasible method to obtain CLIL by cross-linking after adsorbing 
      lipase on SiO(2) nanoparticles.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Qian, Junqing
AU  - Qian J
AD  - College of Pharmaceutical Sciences, Zhejiang University of Technology, 18, 
      Chaowang Road, Hangzhou, 310014, People's Republic of China. 
      junqingqian111@163.com.
FAU - Huang, Aomei
AU  - Huang A
AD  - College of Pharmaceutical Sciences, Zhejiang University of Technology, 18, 
      Chaowang Road, Hangzhou, 310014, People's Republic of China.
FAU - Zhu, Hanxiao
AU  - Zhu H
AD  - College of Pharmaceutical Sciences, Zhejiang University of Technology, 18, 
      Chaowang Road, Hangzhou, 310014, People's Republic of China.
FAU - Ding, Jing
AU  - Ding J
AD  - College of Pharmaceutical Sciences, Zhejiang University of Technology, 18, 
      Chaowang Road, Hangzhou, 310014, People's Republic of China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - College of Pharmaceutical Sciences, Zhejiang University of Technology, 18, 
      Chaowang Road, Hangzhou, 310014, People's Republic of China.
FAU - Chen, Yan
AU  - Chen Y
AD  - College of Pharmaceutical Sciences, Zhejiang University of Technology, 18, 
      Chaowang Road, Hangzhou, 310014, People's Republic of China.
LA  - eng
GR  - KYY-HX-20210856/the Project of Zhejiang University of Technology/
GR  - 2016YFD0400803/the China National Key R&D Program during the 13th Five-year Plan 
      Period/
PT  - Journal Article
DEP - 20221112
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
RN  - 0 (Enzymes, Immobilized)
RN  - T3C89M417N (Glutaral)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - *Enzymes, Immobilized/chemistry
MH  - Glutaral
MH  - Enzyme Stability
MH  - *Silicon Dioxide/chemistry
MH  - Adsorption
MH  - Lipase/chemistry
MH  - Temperature
MH  - Hydrogen-Ion Concentration
OTO - NOTNLM
OT  - Adsorption
OT  - Cross-linking
OT  - Glutaraldehyde
OT  - Lipase
OT  - SiO2 nanoparticles
EDAT- 2022/11/13 06:00
MHDA- 2023/01/07 06:00
CRDT- 2022/11/12 11:14
PHST- 2022/06/30 00:00 [received]
PHST- 2022/10/31 00:00 [accepted]
PHST- 2022/11/13 06:00 [pubmed]
PHST- 2023/01/07 06:00 [medline]
PHST- 2022/11/12 11:14 [entrez]
AID - 10.1007/s00449-022-02810-z [pii]
AID - 10.1007/s00449-022-02810-z [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2023 Jan;46(1):25-38. doi: 10.1007/s00449-022-02810-z. 
      Epub 2022 Nov 12.