PMID- 36370347
OWN - NLM
STAT- MEDLINE
DCOM- 20221115
LR  - 20221115
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2582
DP  - 2023
TI  - Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN 
      Genes Expression Mechanism.
PG  - 103-126
LID - 10.1007/978-1-0716-2744-0_9 [doi]
AB  - Cell communication network factor 2 (CCN2), also known as connective tissue 
      growth factor (CTGF), is protein inducible in response to TGFbeta/Smad signal or the 
      transcriptional activity of matrix metalloproteinase 3 (MMP3). We discovered that 
      MMP3 in exosomes is transferable to recipient cells and then translocates into 
      cell nuclei to transactivate the CCN2/CTGF gene. Exosomes and liposomes enable 
      molecular transfection to recipient cells in vitro and in vivo. These small 
      vesicles are surrounded by lipid membranes and carry proteins, RNA, DNA, and 
      small chemicals. Here we define the exosome-based transfection as "exofection." 
      In addition, spinfection increases the efficiencies of transfection, exofection, 
      and viral infection, thus being compatible with various molecular transfer 
      protocols. Here, we provide protocols, tips, and practical examples of 
      transfection, spinfection, exofection, fluorescence microscopy, and luciferase 
      assays to analyze the CCNs gene expression mechanisms.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Eguchi, Takanori
AU  - Eguchi T
AD  - Department of Dental Pharmacology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan. 
      eguchi@okayama-u.ac.jp.
FAU - Lu, Yanyin
AU  - Lu Y
AD  - Department of Dental Pharmacology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
AD  - Department of Dental Anesthesiology and Special Care Dentistry, Okayama 
      University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 
      Okayama, Japan.
FAU - Taha, Eman A
AU  - Taha EA
AD  - Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, 
      Japan.
AD  - Department of Biochemistry, Ain Shams University Faculty of Science, Cairo, 
      Egypt.
FAU - Okusha, Yuka
AU  - Okusha Y
AD  - Division of Molecular and Cellular Biology, Department of Radiation Oncology, 
      Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - EC 1.13.12.- (Luciferases)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - *Matrix Metalloproteinase 3/genetics/metabolism
MH  - *Signal Transduction/physiology
MH  - Connective Tissue Growth Factor/genetics/metabolism
MH  - Transfection
MH  - Luciferases/genetics/metabolism
MH  - Transforming Growth Factor beta/genetics
OTO - NOTNLM
OT  - Exosomes
OT  - cis-elements
OT  - enhancer
OT  - exofection
OT  - gaussia luciferase
OT  - gene promoter activity
OT  - immunofluorescence
OT  - luciferase assay
OT  - spinfection
OT  - transfection
EDAT- 2022/11/13 06:00
MHDA- 2022/11/16 06:00
CRDT- 2022/11/12 11:21
PHST- 2022/11/12 11:21 [entrez]
PHST- 2022/11/13 06:00 [pubmed]
PHST- 2022/11/16 06:00 [medline]
AID - 10.1007/978-1-0716-2744-0_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2582:103-126. doi: 10.1007/978-1-0716-2744-0_9.