PMID- 36370492
OWN - NLM
STAT- MEDLINE
DCOM- 20221213
LR  - 20221219
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Linking)
VI  - 130
DP  - 2022 Dec
TI  - PVT1/miR-145-5p/HK2 modulates vascular smooth muscle cells phenotype switch via 
      glycolysis: The new perspective on the spiral artery remodeling.
PG  - 25-33
LID - S0143-4004(22)00435-0 [pii]
LID - 10.1016/j.placenta.2022.10.010 [doi]
AB  - INTRODUCTION: Vascular smooth muscle cells (VSMC) switched from a contractile 
      phenotype to a synthetic phenotype during the decidual spiral artery (SPAs) 
      remodeling process. The lncRNA plasmacytoma variant translocation 1 (PVT1) and 
      glucose metabolism have been found to regulate the VSMC phenotype switch. This 
      study aimed to analyze the dynamic expression of PVT1 and glycolytic key enzymes 
      hexokinase2 (HK2) at different remodeling stages in early human pregnancy and 
      elucidate the underlying mechanism of the PVT1/miR-145-5p/HK2 axis involved in 
      the spiral artery remodeling. METHODS: qRT-PCR, Western blot (WB) analysis, 
      Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) were 
      used to detect the expression and localization of PVT1 and HK2 in decidual 
      tissue. HA-VSMCs were transfected with specific siRNA, shRNA and plasmids to 
      regulate corresponding genes. Extracellular lactate, cellular ATP, ROS, and 
      intracellular NADPH levels were measured using the corresponding assay kits. 
      Migration was measured by wound-healing and Transwell assays. Contractile 
      phenotypic markers alpha-SMA, MYH11 with calponin and synthetic phenotypic markers 
      OPN and vimentin were detected by WB. The PDC model was used to detect the degree 
      of spiral arterial remodeling. RESULTS: PVT1 and HK2 were upregulated with 
      gestational age (GA) increasing in decidual tissue during the early pregnancy. 
      HK2 regulated the glycolytic activity and VSMC phenotype switch in vitro. PVT1 
      regulated the glycolytic activity and VSMC phenotype switch through HK2. PVT1 
      played a ceRNA role in regulating HK2 expression by sponging miR-145-5p. PVT1 and 
      HK2 influenced spiral artery remodeling in the PDC model. DISCUSSION: PVT1 and 
      HK2 were upregulated, and miR-145-5p was downregulated in decidua with the GA 
      increasing. Meanwhile, the PVT1/miR-145-5p/HK2 axis may be involved in regulating 
      the phenotypic switch and migratory capacity of VSMCs by affecting glycolysis in 
      decidual SPAs remodeling.
CI  - Copyright (c) 2022. Published by Elsevier Ltd.
FAU - Wu, Mengying
AU  - Wu M
AD  - Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Liu, Weifang
AU  - Liu W
AD  - Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Huang, Haixia
AU  - Huang H
AD  - Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Chen, Zhirui
AU  - Chen Z
AD  - Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Chen, Yangyang
AU  - Chen Y
AD  - Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Zhong, Yanqi
AU  - Zhong Y
AD  - Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Jin, Zhishan
AU  - Jin Z
AD  - Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
FAU - Liu, Xiaoxia
AU  - Liu X
AD  - Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China. Electronic address: 
      xiehesummer@hust.edu.cn.
FAU - Zou, Li
AU  - Zou L
AD  - Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China. Electronic address: 
      xiehezouli@hust.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221018
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN145 microRNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (PVT1 long-non-coding RNA, human)
RN  - EC 2.7.1.1 (HK2 protein, human)
SB  - IM
EIN - Placenta. 2022 Dec 7;131:71-74. PMID: 36495833
MH  - Female
MH  - Humans
MH  - Pregnancy
MH  - Arteries/metabolism
MH  - Cell Proliferation/genetics
MH  - Glycolysis/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/metabolism
MH  - Muscle, Smooth, Vascular/metabolism
MH  - Myocytes, Smooth Muscle/metabolism
MH  - Phenotype
MH  - *RNA, Long Noncoding/genetics
MH  - RNA, Small Interfering
OTO - NOTNLM
OT  - Glycolysis
OT  - HK2
OT  - PVT1
OT  - Spiral artery remodeling
OT  - VSMC phenotype Switch
COIS- Declaration of competing interest All authors declare no conflict of interest.
EDAT- 2022/11/13 06:00
MHDA- 2022/12/07 06:00
CRDT- 2022/11/12 18:15
PHST- 2022/05/02 00:00 [received]
PHST- 2022/10/01 00:00 [revised]
PHST- 2022/10/14 00:00 [accepted]
PHST- 2022/11/13 06:00 [pubmed]
PHST- 2022/12/07 06:00 [medline]
PHST- 2022/11/12 18:15 [entrez]
AID - S0143-4004(22)00435-0 [pii]
AID - 10.1016/j.placenta.2022.10.010 [doi]
PST - ppublish
SO  - Placenta. 2022 Dec;130:25-33. doi: 10.1016/j.placenta.2022.10.010. Epub 2022 Oct 
      18.