PMID- 36372265
OWN - NLM
STAT- MEDLINE
DCOM- 20221220
LR  - 20221222
IS  - 1096-0295 (Electronic)
IS  - 0273-2300 (Linking)
VI  - 137
DP  - 2023 Jan
TI  - Feasibility study for a downsized comparative thyroid assay with measurement of 
      brain thyroid hormones and histopathology in rats: Case study with 
      6-propylthiouracil and sodium phenobarbital at high dose.
PG  - 105283
LID - S0273-2300(22)00170-2 [pii]
LID - 10.1016/j.yrtph.2022.105283 [doi]
AB  - Concern has been raised that thyroid hormone disruptors (THDs) may potentially 
      interfere with the developing brain, but effects of mild suppression of maternal 
      THs by environmental contaminants on neonatal brain development are not fully 
      understood. The comparative thyroid assay (CTA) is a screening test for offspring 
      THDs, but it requires several animals and is criticized that reliance on serum 
      THs alone as predictive markers of brain malfunction is inadequate. To verify 
      feasibility of the downsized CTA but additional examination of brain THs levels 
      and histopathology, we commenced internal-validation studies. This paper presents 
      the data of the study where 6-propylthiouracil (6-PTU, 10 ppm) and sodium 
      phenobarbital (NaPB, 1000 ppm) were dosed by feeding from gestational days 
      (GD)6-20, and from GD6 to lactation day 21. The modified CTA detected 
      6-PTU-induced severe (>70%) suppression of serum THs in dams, with >50% 
      suppressed serum/brain TH levels in offspring and brain heterotopia in postnatal 
      day 21 pups. The modified CTA also detected NaPB-induced mild (<35%) suppression 
      of serum THs in dams, with mild (<35%) reduction of serum/brain TH levels in 
      fetuses but not in pups. These findings suggest that the modified CTA may have a 
      potential as a screening test for offspring THDs.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Minami, Kenta
AU  - Minami K
AD  - Environmental Health Science Laboratory, Sumitomo Chemical Company, Ltd., 3-1-98, 
      Kasugade-naka 3-chome, Konohana-ku, Osaka, 554-8558, Japan.
FAU - Suto, Hidenori
AU  - Suto H
AD  - Environmental Health Science Laboratory, Sumitomo Chemical Company, Ltd., 3-1-98, 
      Kasugade-naka 3-chome, Konohana-ku, Osaka, 554-8558, Japan.
FAU - Sato, Akira
AU  - Sato A
AD  - Institute of Environmental Toxicology, 4321, Uchimoriya-machi, Joso-shi, Ibaraki, 
      303-0043, Japan.
FAU - Ogata, Keiko
AU  - Ogata K
AD  - Environmental Health Science Laboratory, Sumitomo Chemical Company, Ltd., 3-1-98, 
      Kasugade-naka 3-chome, Konohana-ku, Osaka, 554-8558, Japan.
FAU - Kosaka, Tadashi
AU  - Kosaka T
AD  - Institute of Environmental Toxicology, 4321, Uchimoriya-machi, Joso-shi, Ibaraki, 
      303-0043, Japan.
FAU - Hojo, Hitoshi
AU  - Hojo H
AD  - Institute of Environmental Toxicology, 4321, Uchimoriya-machi, Joso-shi, Ibaraki, 
      303-0043, Japan.
FAU - Takahashi, Naofumi
AU  - Takahashi N
AD  - Institute of Environmental Toxicology, 4321, Uchimoriya-machi, Joso-shi, Ibaraki, 
      303-0043, Japan.
FAU - Tomiyama, Naruto
AU  - Tomiyama N
AD  - Institute of Environmental Toxicology, 4321, Uchimoriya-machi, Joso-shi, Ibaraki, 
      303-0043, Japan.
FAU - Fukuda, Takako
AU  - Fukuda T
AD  - Bioscience Research Laboratory, Sumitomo Chemical Company, Ltd., 3-1-98 
      Kasugade-naka, Konohana-ku, Osaka, 554-8558, Japan.
FAU - Iwashita, Katsumasa
AU  - Iwashita K
AD  - Environmental Health Science Laboratory, Sumitomo Chemical Company, Ltd., 3-1-98, 
      Kasugade-naka 3-chome, Konohana-ku, Osaka, 554-8558, Japan.
FAU - Aoyama, Hiroaki
AU  - Aoyama H
AD  - Institute of Environmental Toxicology, 4321, Uchimoriya-machi, Joso-shi, Ibaraki, 
      303-0043, Japan.
FAU - Yamada, Tomoya
AU  - Yamada T
AD  - Environmental Health Science Laboratory, Sumitomo Chemical Company, Ltd., 3-1-98, 
      Kasugade-naka 3-chome, Konohana-ku, Osaka, 554-8558, Japan. Electronic address: 
      yamadat8@sc.sumitomo-chem.co.jp.
LA  - eng
PT  - Journal Article
DEP - 20221111
PL  - Netherlands
TA  - Regul Toxicol Pharmacol
JT  - Regulatory toxicology and pharmacology : RTP
JID - 8214983
RN  - 721M9407IY (Propylthiouracil)
RN  - 0 (Thyroid Hormones)
RN  - YQE403BP4D (Phenobarbital)
RN  - 9NEZ333N27 (Sodium)
SB  - IM
MH  - Female
MH  - Animals
MH  - Rats
MH  - *Propylthiouracil/toxicity
MH  - *Thyroid Gland
MH  - Feasibility Studies
MH  - Thyroid Hormones
MH  - Phenobarbital/pharmacology
MH  - Brain
MH  - Sodium/pharmacology
OTO - NOTNLM
OT  - 6-Propylthiouracil
OT  - Comparative thyroid assay
OT  - Developmental neurotoxicity
OT  - Endocrine disruption
OT  - Heterotopia
OT  - Hypothyroidism
OT  - Screening
OT  - Sodium phenobarbital
OT  - Thyroid hormone
OT  - Uridine diphosphate glucuronyltransferase (UGT)
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/11/14 06:00
MHDA- 2022/12/21 06:00
CRDT- 2022/11/13 19:33
PHST- 2022/06/14 00:00 [received]
PHST- 2022/10/12 00:00 [revised]
PHST- 2022/10/29 00:00 [accepted]
PHST- 2022/11/14 06:00 [pubmed]
PHST- 2022/12/21 06:00 [medline]
PHST- 2022/11/13 19:33 [entrez]
AID - S0273-2300(22)00170-2 [pii]
AID - 10.1016/j.yrtph.2022.105283 [doi]
PST - ppublish
SO  - Regul Toxicol Pharmacol. 2023 Jan;137:105283. doi: 10.1016/j.yrtph.2022.105283. 
      Epub 2022 Nov 11.