PMID- 36372432
OWN - NLM
STAT- MEDLINE
DCOM- 20221115
LR  - 20230104
IS  - 1347-4405 (Electronic)
IS  - 1342-6311 (Print)
IS  - 1342-6311 (Linking)
VI  - 37
IP  - 4
DP  - 2022
TI  - Novel Cross-domain Symbiosis between Candidatus Patescibacteria and 
      Hydrogenotrophic Methanogenic Archaea Methanospirillum Discovered in a 
      Methanogenic Ecosystem.
LID - 10.1264/jsme2.ME22063 [doi]
LID - ME22063
AB  - To identify novel cross-domain symbiosis between Candidatus Patescibacteria and 
      Archaea, we performed fluorescence in situ hybridization (FISH) on enrichment 
      cultures derived from methanogenic bioreactor sludge with the newly designed 
      32-520-1066 probe targeting the family-level uncultured clade 32-520/UBA5633 
      lineage in the class Ca. Paceibacteria. All FISH-detectable 32-520/UBA5633 cells 
      were attached to Methanospirillum, indicating high host specificity. Transmission 
      electron microscopy observations revealed 32-520/UBA5633-like cells that were 
      specifically adherent to the plug structure of Methanospirillum-like rod-shaped 
      cells. The metagenome-assembled genomes of 32-520/UBA5633 encoded unique gene 
      clusters comprising pilin signal peptides and type IV pilins. These results 
      provide novel insights into unseen symbiosis between Ca. Patescibacteria and 
      Archaea.
FAU - Kuroda, Kyohei
AU  - Kuroda K
AD  - Bioproduction Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST).
FAU - Kubota, Kengo
AU  - Kubota K
AD  - Department of Frontier Sciences for Advanced Environment, Graduate School of 
      Environmental Studies, Tohoku University.
AD  - Department of Civil and Environmental Engineering, Graduate School of 
      Engineering, Tohoku University.
FAU - Kagemasa, Shuka
AU  - Kagemasa S
AD  - Bioproduction Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST).
AD  - Department of Civil and Environmental Engineering, Graduate School of 
      Engineering, Tohoku University.
FAU - Nakai, Ryosuke
AU  - Nakai R
AD  - Bioproduction Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST).
FAU - Hirakata, Yuga
AU  - Hirakata Y
AD  - Bioproduction Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST).
FAU - Yamamoto, Kyosuke
AU  - Yamamoto K
AD  - Bioproduction Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST).
FAU - Nobu, Masaru K
AU  - Nobu MK
AD  - Bioproduction Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST).
FAU - Narihiro, Takashi
AU  - Narihiro T
AD  - Bioproduction Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST).
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Microbes Environ
JT  - Microbes and environments
JID - 9710937
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - *Archaea/genetics
MH  - Methanospirillum/genetics
MH  - Symbiosis
MH  - Ecosystem
MH  - In Situ Hybridization, Fluorescence
MH  - Phylogeny
MH  - *Euryarchaeota
MH  - Bacteria/genetics
MH  - RNA, Ribosomal, 16S/genetics
PMC - PMC9763046
OTO - NOTNLM
OT  - 32-520/UBA5633
OT  - Candidate Phyla Radiation (CPR) superphylum
OT  - Candidatus Patescibacteria
OT  - Methanospirillum
OT  - cross-domain symbiosis
EDAT- 2022/11/14 06:00
MHDA- 2022/11/16 06:00
PMCR- 2022/01/01
CRDT- 2022/11/13 20:23
PHST- 2022/11/13 20:23 [entrez]
PHST- 2022/11/14 06:00 [pubmed]
PHST- 2022/11/16 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - DN/JST.JSTAGE/jsme2/ME22063 [pii]
AID - 10.1264/jsme2.ME22063 [doi]
PST - ppublish
SO  - Microbes Environ. 2022;37(4):ME22063. doi: 10.1264/jsme2.ME22063.