PMID- 36373505 OWN - NLM STAT- MEDLINE DCOM- 20221201 LR - 20221201 IS - 2042-650X (Electronic) IS - 2042-6496 (Linking) VI - 13 IP - 23 DP - 2022 Nov 28 TI - Daphnetin contributes to allergen-induced Th2 cytokine expression and type 2-immune responses in atopic dermatitis and asthma. PG - 12383-12399 LID - 10.1039/d2fo02518c [doi] AB - Daphne koreana Nakai is a cherished medicinal plant in the Changbai Mountain region of China. It can be incorporated into medicinal meals and used for various skin diseases by infiltrating liquor. Daphnetin (7,8-dihydroxycoumarin, Dap.) is a main constituent of D. koreana Nakai, which has been used to treat inflammatory conditions and immune disorders due to its numerous pharmacological activities, including anti-oxidant, anti-inflammatory, analgesic, etc. Atopic dermatitis (AD) and allergic asthma are typical diseases of type 2-immune responses. In the present study, the therapeutic potential of Dap. against AD and allergic asthma was investigated using animal and cell experiments. AD-like lesions were induced by repeated application of 1-chloro-2,4-dinitrobenzene (DNCB) to the shaved dorsal skin of BALB/c mice. Ovalbumin (OVA) induction was utilized to establish a mouse asthma model. A passive cutaneous anaphylaxis (PCA) mouse ear model and immunoglobulin E (IgE)/bovine serum albumin (BSA)-stimulated RBL-2H3 cells were used for in vitro assays. The skin lesions and serum and tissue homogenates of the mice were analyzed using histological analysis, immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA), respectively, in order to investigate the anti-AD effects of Dap. Histological analysis was performed on the allergic asthma model to observe inflammatory cell infiltration in the lung tissues. Total IgE and OVA-specific IgE in the serum were measured by ELISA. The levels of inflammatory cytokines in BALF were detected by ELISA. In addition, ELISA and western blotting were performed for the in vitro analysis of RBL-2H3 cells. The results showed that Dap. inhibited the development of DNCB-induced AD-like lesions in the BALB/c mice by reducing the severity of the lesions, epidermal thickness and mast cell infiltration; this was accompanied by reduced levels of IgE and inflammatory cytokines [interleukin (IL)-4, IL-5, IL-9, IL-13, IL-33 and thymic stromal lymphopoietin (TSLP)]. In the allergic asthma model, Dap. reduced the number of infiltrated inflammatory cells in the lung tissues. Moreover, the levels of total serum IgE and OVA-specific IgE were reduced in the high daphnetin dose groups (Dap., -100 mg kg(-1)). Dap. administered at a dose of -100 mg kg(-1) decreased the levels of inflammatory cytokines (IL-4, IL-5, IL-9, IL-13, IL-33 and TSLP in BALF). Furthermore, Dap. administered to IgE-sensitized mice effectively attenuated the IgE-triggered PCA reaction. In vitro, Dap. decreased the expression levels of histamine, IL-4, IL-6, IL-13, MIP-1alpha and INF-alpha, and reduced the protein expression levels of phosphorylated MAPKs, P-Lyn and P-syk in the RBL-2H3 cells. Therefore, Dap. can be represented as a potential therapeutic strategy for the treatment of allergic inflammatory conditions via immunoregulation. FAU - Zhang, Yi AU - Zhang Y AD - School of Pharmaceutical Science, Department of Pharmacology of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. 14030@ynutcm.edu.cn. FAU - Qu, Lu AU - Qu L AD - School of Pharmaceutical Science, Department of Pharmacology of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. 14030@ynutcm.edu.cn. FAU - Sun, Yun AU - Sun Y AUID- ORCID: 0000-0001-6903-547X AD - School of Pharmaceutical Science, Department of Pharmacology of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. 14030@ynutcm.edu.cn. FAU - Lin, YuPing AU - Lin Y AUID- ORCID: 0000-0001-7635-2238 AD - School of Pharmaceutical Science, Department of Pharmacology of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. 14030@ynutcm.edu.cn. FAU - Zeng, Jing AU - Zeng J AD - Department of Chinese Medicine, College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - He, LiXia AU - He L AD - The Second Affiliated Hospital of Wannan Medical College, Wuhu 241001, China. FAU - Li, Xiucun AU - Li X AD - Qi-Lu Hospital of Shandong University, Jinan 250012, China. FAU - Gu, Wen AU - Gu W AD - School of Pharmaceutical Science, Department of Pharmacology of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. 14030@ynutcm.edu.cn. FAU - Nie, Jian AU - Nie J AD - School of Pharmaceutical Science, Department of Pharmacology of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. 14030@ynutcm.edu.cn. FAU - Yu, Xiaoling AU - Yu X AD - The Third Affiliated Hospital of Yunnan University of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650021, China. FAU - Tong, XiaoYun AU - Tong X AUID- ORCID: 0000-0002-8722-4706 AD - The First Affiliated Hospital of Yunnan University of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650021, China. 08003@ynutcm.edu.cn. FAU - Huang, Feng AU - Huang F AUID- ORCID: 0000-0002-8741-4219 AD - School of Pharmaceutical Science, Department of Pharmacology of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. 14030@ynutcm.edu.cn. AD - Yunnan Key Laboratory of Southern Medicinal Utilization, College of Traditional, Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650021, China. LA - eng PT - Journal Article DEP - 20221128 PL - England TA - Food Funct JT - Food & function JID - 101549033 RN - 0 (Allergens) RN - 0 (Anti-Inflammatory Agents) RN - 0 (Cytokines) RN - XC84571RD2 (daphnetin) RN - 0 (Dinitrochlorobenzene) RN - 37341-29-0 (Immunoglobulin E) RN - 0 (Interleukin-13) RN - 0 (Interleukin-33) RN - 207137-56-2 (Interleukin-4) RN - 0 (Interleukin-5) RN - 0 (Interleukin-9) RN - 0 (Umbelliferones) SB - IM MH - Animals MH - Mice MH - Allergens/adverse effects MH - *Anti-Inflammatory Agents/therapeutic use MH - *Asthma/chemically induced/drug therapy MH - Cytokines/metabolism MH - *Dermatitis, Atopic/drug therapy MH - Dinitrochlorobenzene/adverse effects MH - Disease Models, Animal MH - Immunity MH - Immunoglobulin E MH - Interleukin-13 MH - Interleukin-33 MH - Interleukin-4 MH - Interleukin-5 MH - Interleukin-9 MH - Mice, Inbred BALB C MH - *Umbelliferones/therapeutic use EDAT- 2022/11/15 06:00 MHDA- 2022/12/01 06:00 CRDT- 2022/11/14 04:42 PHST- 2022/11/15 06:00 [pubmed] PHST- 2022/12/01 06:00 [medline] PHST- 2022/11/14 04:42 [entrez] AID - 10.1039/d2fo02518c [doi] PST - epublish SO - Food Funct. 2022 Nov 28;13(23):12383-12399. doi: 10.1039/d2fo02518c.