PMID- 36378420
OWN - NLM
STAT- MEDLINE
DCOM- 20230202
LR  - 20230202
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 50
IP  - 2
DP  - 2023 Feb
TI  - Analyses of P16(INK4a) gene promoter methylation relative to molecular, 
      demographic and clinical parameters characteristics in non-small cell lung cancer 
      patients: A pilot study.
PG  - 971-979
LID - 10.1007/s11033-022-07982-1 [doi]
AB  - BACKGROUND: The aim of this study was to examine the methylation status of 
      p16(INK4a) promoter region in non small cell lung cancer (NSCLC) patients and 
      their associations with single nucleotide polymorphisms (SNPs) of the epidermal 
      growth factor receptor (EGFR) gene, as well as with demographic or clinical 
      characteristics. METHODS: Formalin-fixed and paraffin-embedded (FFPE) DNA samples 
      extracted from 22 NSCLC patients were analyzed with methylation-specific 
      polymerase chain reaction (PCR) method to obtain promoter methylation profile. 
      The same cohort was genotyped for - 216G > T, -191 C > A, and 181,946 C > T EGFR 
      SNPs. RESULTS: There was a significant association between methylated p16(INK4a) 
      in patients prior therapy (p = 0.017) since a significantly higher frequency of 
      methylated p16(INK4a) was detected in these patients (40.9%) in comparison to 
      frequency in patients after therapy (31.8%). Also, a higher frequency of 
      methylated p16(INK4a) was detected among patients with leucopenia (p = 0.056). No 
      associations were observed between the methylation status of the p16(INK4a) 
      promoter region and EGFR SNPs or other clinical and demographic data in this 
      cohort. CONCLUSION: High frequency of methylation of the p16(INK4a) gene promoter 
      was observed in NSCLC patients prior therapy and with leucopenia that can 
      indicate their significance related to advanced clinical stage.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Jurisic, Vladimir
AU  - Jurisic V
AD  - Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 
      34000, Kragujevac, Serbia. jurisicvladimir@gmail.com.
FAU - Obradovic, Jasmina
AU  - Obradovic J
AD  - Department of Sciences, University of Kragujevac, Institute for Information 
      Technologies, 34000, Kragujevac, Serbia.
FAU - Nikolic, Nadja
AU  - Nikolic N
AD  - Department of Human Genetics, School of Dental Medicine, University of Belgrade, 
      11000, Belgrade, Serbia.
FAU - Javorac, Jovan
AU  - Javorac J
AD  - Institute for Pulmonary Diseases of Vojvodina, 21000, Sremska Kamenica, Serbia.
FAU - Perin, Branislav
AU  - Perin B
AD  - Institute for Pulmonary Diseases of Vojvodina, 21000, Sremska Kamenica, Serbia.
FAU - Milasin, Jelena
AU  - Milasin J
AD  - Department of Human Genetics, School of Dental Medicine, University of Belgrade, 
      11000, Belgrade, Serbia.
LA  - eng
GR  - 175056/Ministarstvo Prosvete, Nauke i Tehnoloskog Razvoja/
PT  - Journal Article
DEP - 20221115
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Humans
MH  - *Carcinoma, Non-Small-Cell Lung/genetics
MH  - Cyclin-Dependent Kinase Inhibitor p16/genetics/metabolism
MH  - Pilot Projects
MH  - *Lung Neoplasms/genetics
MH  - DNA Methylation/genetics
MH  - Promoter Regions, Genetic/genetics
MH  - ErbB Receptors/genetics/metabolism
MH  - Demography
OTO - NOTNLM
OT  - EGFR
OT  - Lung cancer
OT  - Methylation
OT  - SNPs
OT  - p16INK4a
EDAT- 2022/11/16 06:00
MHDA- 2023/02/03 06:00
CRDT- 2022/11/15 11:38
PHST- 2022/07/22 00:00 [received]
PHST- 2022/09/23 00:00 [accepted]
PHST- 2022/11/16 06:00 [pubmed]
PHST- 2023/02/03 06:00 [medline]
PHST- 2022/11/15 11:38 [entrez]
AID - 10.1007/s11033-022-07982-1 [pii]
AID - 10.1007/s11033-022-07982-1 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2023 Feb;50(2):971-979. doi: 10.1007/s11033-022-07982-1. Epub 2022 
      Nov 15.