PMID- 36379195
OWN - NLM
STAT- MEDLINE
DCOM- 20221207
LR  - 20221207
IS  - 1878-562X (Electronic)
IS  - 1567-5394 (Linking)
VI  - 149
DP  - 2023 Feb
TI  - An electrochemical apta-assay based on hybridization chain reaction and aflatoxin 
      B1-driven Ag-DNAzyme as amplification strategy.
PG  - 108322
LID - S1567-5394(22)00273-0 [pii]
LID - 10.1016/j.bioelechem.2022.108322 [doi]
AB  - Herein, a novel electrochemical apta-assay based on hybridization chain reaction 
      (HCR) and aflatoxin B1-driven Ag-DNAzyme was prepared. The combination of HCR and 
      Ag-DNAzyme was designed for the first time as a dual signal amplification 
      strategy for the detection of mycotoxins. The substrate DNA (sDNA) was fixed to 
      the electrode surface, which contained the RNA A (rA) site and HCR initiation 
      sequence. The sDNA opened the hairpin DNA structures and triggered a cascade of 
      hybridization events. The DNA double strands formed by the HCR bound large 
      amounts of methylene blue (MB). Aptamer and complementary DNA bound to Ag(+) by 
      C-Ag(+)-C complexes. AFB1 can drive Ag(+) shedding, and Ag(+) induced Ag-DNAzyme 
      to shear the rA site of sDNA. The amount of binding MB decreased and the current 
      signal decreased. Replaced biological enzymes with metal ion-mediated DNAzyme 
      enhanced the stability of the prepared sensors while reducing the preparation 
      cost. An adequate determination of AFB1 in corn flour, walnut powder, and other 
      actual samples are validated, which indicated the good accuracy and potential 
      application in real samples. The strategy is characterized by simple operation, 
      good stability, and low preparation cost, and has good application prospects in 
      food safety and quality control.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Liu, Jiahui
AU  - Liu J
AD  - College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil 
      Food Safety Inspection and Control, Henan University of Technology, Zhengzhou 
      450001, PR China.
FAU - Suo, Zhiguang
AU  - Suo Z
AD  - College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil 
      Food Safety Inspection and Control, Henan University of Technology, Zhengzhou 
      450001, PR China.
FAU - Liu, Yong
AU  - Liu Y
AD  - College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, 
      PR China.
FAU - He, Baoshan
AU  - He B
AD  - College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil 
      Food Safety Inspection and Control, Henan University of Technology, Zhengzhou 
      450001, PR China.
FAU - Wei, Min
AU  - Wei M
AD  - College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil 
      Food Safety Inspection and Control, Henan University of Technology, Zhengzhou 
      450001, PR China. Electronic address: wei_min80@163.com.
LA  - eng
PT  - Journal Article
DEP - 20221111
PL  - Netherlands
TA  - Bioelectrochemistry
JT  - Bioelectrochemistry (Amsterdam, Netherlands)
JID - 100953583
RN  - 0 (DNA, Catalytic)
RN  - 9N2N2Y55MH (Aflatoxin B1)
RN  - 9007-49-2 (DNA)
RN  - T42P99266K (Methylene Blue)
RN  - 0 (Aptamers, Nucleotide)
SB  - IM
MH  - *DNA, Catalytic/chemistry
MH  - Aflatoxin B1/analysis
MH  - Electrochemical Techniques
MH  - *Biosensing Techniques
MH  - DNA/chemistry
MH  - Methylene Blue/chemistry
MH  - Limit of Detection
MH  - *Aptamers, Nucleotide/chemistry
OTO - NOTNLM
OT  - Aflatoxin B1
OT  - Ag-DNAzyme
OT  - Electrochemical apta-assay
OT  - Hybridization chain reaction
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/11/16 06:00
MHDA- 2023/02/25 06:00
CRDT- 2022/11/15 18:27
PHST- 2022/09/03 00:00 [received]
PHST- 2022/10/26 00:00 [revised]
PHST- 2022/11/07 00:00 [accepted]
PHST- 2022/11/16 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
PHST- 2022/11/15 18:27 [entrez]
AID - S1567-5394(22)00273-0 [pii]
AID - 10.1016/j.bioelechem.2022.108322 [doi]
PST - ppublish
SO  - Bioelectrochemistry. 2023 Feb;149:108322. doi: 10.1016/j.bioelechem.2022.108322. 
      Epub 2022 Nov 11.