PMID- 36380877
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221117
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Print)
IS  - 1792-1074 (Linking)
VI  - 24
IP  - 6
DP  - 2022 Dec
TI  - Potential of blood exosomal ENAH, SEPT9, EGF, MMP-9 and CXCL8 for the early 
      screening of breast cancer.
PG  - 460
LID - 10.3892/ol.2022.13580 [doi]
LID - 460
AB  - Exosomal contents have been recognized as candidate biomarkers for cancer 
      screening and prognosis. The current study aimed to evaluate the potential of the 
      expression levels of exosomal enabled homolog (ENAH), septin 9 (SEPT9), epidermal 
      growth factor (EGF), matrix metalloproteinase-9 (MMP-9) and C-X-C motif chemokine 
      ligand 8 (CXCL8) in the blood for the early screening of breast cancer. 
      Therefore, exosomes were extracted and purified from the peripheral blood of 47 
      patients with breast cancer, 63 disease controls (DCs) and 33 healthy controls 
      (HCs). Subsequently, the exosomal mRNA expression levels of ENAH, SEPT9, EGF, 
      MMP-9 and CXCL8 were detected by reverse transcription-quantitative polymerase 
      chain reaction. The results showed that the exosomal levels of ENAH and EGF were 
      significantly higher in patients with breast cancer compared with DCs and HCs 
      (both P<0.001). In addition, receiver operating characteristic curves revealed 
      that exosomal ENAH was able to discriminate patients with breast cancer from DCs 
      [area under the curve (AUC), 0.841] and HCs (AUC, 0.859). However, exosomal EGF 
      was only able to discriminate patients with breast cancer from HCs (AUC, 0.776). 
      Furthermore, the levels of exosomal SEPT9 were lower in patients with breast 
      cancer compared with DCs and HCs (P=0.021), and exosomal SEPT9 expression levels 
      exhibited good potential in the discrimination of patients with breast cancer 
      from DCs (AUC, 0.717) and HCs (AUC, 0.830). However, no significant difference 
      was detected in exosomal levels of MMP-9 and CXCL8 among the three groups, and 
      these RNAs showed no discriminative ability. In addition, in patients with breast 
      cancer, the exosomal levels of ENAH were associated with molecular subtypes 
      (P=0.010), while those of MMP-9 were associated with a Ki-67 index of >/=30% 
      (P=0.011). In conclusion, the exosomal levels of ENAH, SEPT9 and EGF in blood 
      samples were able to identify patients with breast cancer, thus providing a novel 
      approach for the early screening of breast cancer.
CI  - Copyright: (c) Zhang et al.
FAU - Zhang, Zijing
AU  - Zhang Z
AD  - Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 
      200040, P.R. China.
FAU - Wang, Hongying
AU  - Wang H
AD  - Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 
      200040, P.R. China.
FAU - Jin, Yiting
AU  - Jin Y
AD  - Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 
      200040, P.R. China.
FAU - Chu, Chengyu
AU  - Chu C
AD  - Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 
      200040, P.R. China.
FAU - Bai, Jinsong
AU  - Bai J
AD  - Department of Clinical Medicine, Shanghai Medical College, Fudan University, 
      Shanghai 200433, P.R. China.
FAU - Huang, Juntian
AU  - Huang J
AD  - Department of Clinical Medicine, Shanghai Medical College, Fudan University, 
      Shanghai 200433, P.R. China.
FAU - Yang, Lemei
AU  - Yang L
AD  - Department of Pathology, School of Basic Medical Sciences, Fudan University, 
      Shanghai 200032, P.R. China.
FAU - Tang, Feng
AU  - Tang F
AD  - Department of Pathology, Huashan Hospital, Fudan University, Shanghai 200040, 
      P.R. China.
FAU - Zou, Liping
AU  - Zou L
AD  - Department of Pathology, Huashan Hospital, Fudan University, Shanghai 200040, 
      P.R. China.
FAU - Wang, Shuyang
AU  - Wang S
AD  - Department of Pathology, School of Basic Medical Sciences, Fudan University, 
      Shanghai 200032, P.R. China.
FAU - Zou, Qiang
AU  - Zou Q
AD  - Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 
      200040, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20221103
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC9650598
OTO - NOTNLM
OT  - breast cancer
OT  - early screening
OT  - exosomal EGF
OT  - exosomal ENAH
OT  - exosomal SEPT9
COIS- The authors declare that they have no competing interests.
EDAT- 2022/11/17 06:00
MHDA- 2022/11/17 06:01
PMCR- 2022/11/03
CRDT- 2022/11/16 02:45
PHST- 2022/07/29 00:00 [received]
PHST- 2022/09/28 00:00 [accepted]
PHST- 2022/11/16 02:45 [entrez]
PHST- 2022/11/17 06:00 [pubmed]
PHST- 2022/11/17 06:01 [medline]
PHST- 2022/11/03 00:00 [pmc-release]
AID - OL-24-06-13580 [pii]
AID - 10.3892/ol.2022.13580 [doi]
PST - epublish
SO  - Oncol Lett. 2022 Nov 3;24(6):460. doi: 10.3892/ol.2022.13580. eCollection 2022 
      Dec.