PMID- 36381632
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221117
IS  - 2228-5652 (Print)
IS  - 2228-5660 (Electronic)
IS  - 2228-5652 (Linking)
VI  - 12
IP  - 5
DP  - 2022
TI  - CFP10-loaded PLGA nanoparticles as a booster vaccine confer protective immunity 
      against Mycobacterium bovis.
PG  - 395-404
LID - 10.34172/bi.2022.23645 [doi]
AB  - Introduction: The limited efficacy of BCG (bacillus Calmette-Guerin) urgently 
      requires new effective vaccination approaches for the control of tuberculosis. 
      Poly lactic-co-glycolic acid (PLGA) is a prevalent drug delivery system. However, 
      the effect of PLGA-based nanoparticles (NPs) against tuberculosis for the 
      induction of mucosal immune response is no fully elucidated. In this study, we 
      hypothesized that intranasal immunization with culture filtrate protein-10 
      (CFP10)-loaded PLGA NPs (CFP10-NPs) could boost the protective immunity of BCG 
      against Mycobacterium bovis in mice. Methods: The recombinant protein CFP10 was 
      encapsulated with PLGA NPs to prepare CFP10-NPs by the classical water-oil-water 
      solvent-evaporation method. Then, the immunoregulatory effects of CFP10-NPs on 
      macrophages in vitro and on BCG-immunized mice in vivo were investigated. 
      Results: We used spherical CFP10-NPs with a negatively charged surface 
      (zeta-potential -28.5 +/- 1.7 mV) having a particle size of 281.7 +/- 28.5 nm in 
      diameter. Notably, CFP10-NPs significantly enhanced the secretion of tumor 
      necrosis factor alpha (TNF-alpha) and interleukin (IL)-1beta in J774A.1 macrophages. 
      Moreover, mucosal immunization with CFP10-NPs significantly increased TNF-alpha and 
      IL-1beta production in serum, and immunoglobulin A (IgA) secretion in 
      bronchoalveolar lavage fluid (BALF), and promoted the secretion of CFP10-specific 
      interferon-gamma (IFN-gamma) in splenocytes of mice. Furthermore, CFP10-NPs immunization 
      significantly reduced the inflammatory area and bacterial load in lung tissues at 
      3-week post-M. bovis challenge. Conclusion: CFP10-NPs markedly improve the 
      immunogenicity and protective efficacy of BCG. Our findings explore the potential 
      of the airway mucosal vaccine based on PLGA NPs as a vehicle for targeted lung 
      delivery.
CI  - (c) 2022 The Author(s).
FAU - Liang, Zhengmin
AU  - Liang Z
AUID- ORCID: 0000-0001-6473-4511
AD  - Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, 
      National Animal Transmissible Spongiform Encephalopathy Laboratory, College of 
      Veterinary Medicine, China Agricultural University, 100193, Beijing, China.
FAU - Li, Miaoxuan
AU  - Li M
AD  - Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, 
      National Animal Transmissible Spongiform Encephalopathy Laboratory, College of 
      Veterinary Medicine, China Agricultural University, 100193, Beijing, China.
FAU - Ni, Jiamin
AU  - Ni J
AD  - Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, 
      National Animal Transmissible Spongiform Encephalopathy Laboratory, College of 
      Veterinary Medicine, China Agricultural University, 100193, Beijing, China.
FAU - Hussain, Tariq
AU  - Hussain T
AD  - Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, 
      National Animal Transmissible Spongiform Encephalopathy Laboratory, College of 
      Veterinary Medicine, China Agricultural University, 100193, Beijing, China.
FAU - Yao, Jiao
AU  - Yao J
AD  - Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, 
      National Animal Transmissible Spongiform Encephalopathy Laboratory, College of 
      Veterinary Medicine, China Agricultural University, 100193, Beijing, China.
FAU - Song, Yinjuan
AU  - Song Y
AD  - Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, 
      National Animal Transmissible Spongiform Encephalopathy Laboratory, College of 
      Veterinary Medicine, China Agricultural University, 100193, Beijing, China.
FAU - Liu, Yiduo
AU  - Liu Y
AD  - Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, 
      National Animal Transmissible Spongiform Encephalopathy Laboratory, College of 
      Veterinary Medicine, China Agricultural University, 100193, Beijing, China.
FAU - Wang, Haoran
AU  - Wang H
AD  - Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, 
      National Animal Transmissible Spongiform Encephalopathy Laboratory, College of 
      Veterinary Medicine, China Agricultural University, 100193, Beijing, China.
FAU - Zhou, Xiangmei
AU  - Zhou X
AUID- ORCID: 0000-0003-1566-5745
AD  - Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, 
      National Animal Transmissible Spongiform Encephalopathy Laboratory, College of 
      Veterinary Medicine, China Agricultural University, 100193, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20220608
PL  - Iran
TA  - Bioimpacts
JT  - BioImpacts : BI
JID - 101558125
PMC - PMC9596879
OTO - NOTNLM
OT  - CFP10
OT  - Mucosal immunization
OT  - Mycobacterium bovis
OT  - Nanoparticles
OT  - PLGA
EDAT- 2022/11/17 06:00
MHDA- 2022/11/17 06:01
PMCR- 2022/01/01
CRDT- 2022/11/16 02:55
PHST- 2021/02/01 00:00 [received]
PHST- 2021/09/25 00:00 [revised]
PHST- 2021/10/20 00:00 [accepted]
PHST- 2022/11/16 02:55 [entrez]
PHST- 2022/11/17 06:00 [pubmed]
PHST- 2022/11/17 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.34172/bi.2022.23645 [doi]
PST - ppublish
SO  - Bioimpacts. 2022;12(5):395-404. doi: 10.34172/bi.2022.23645. Epub 2022 Jun 8.