PMID- 36383801
OWN - NLM
STAT- MEDLINE
DCOM- 20221118
LR  - 20221202
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 55
DP  - 2022
TI  - Nanosized copper particles induced mesangial cell toxicity via the autophagy 
      pathway.
PG  - e12252
LID - S0100-879X2022000100674 [pii]
LID - 10.1590/1414-431X2022e12252 [doi]
LID - e12252
AB  - Nanosized copper particles (nano Cu) have been incorporated into products in 
      multiple industries, although studies have demonstrated that these particles are 
      nephrotoxic. We investigated the cytotoxicity of nanosized copper particles on 
      rat mesangial cells and measured rates of apoptosis, the expression of caspase-3, 
      and generation of reactive oxygen species. We also measured autophagy through the 
      acridine orange (AO) staining and expression of Beclin-1, microtubule-associated 
      protein 1 light chain 3, and p62 to screen the underlying mechanism of toxicity. 
      Nanosized copper particles inhibited mesangial cell viability, up-regulated the 
      activity of caspase-3, and increased the rates of apoptosis and the generation of 
      reactive oxygen species in a concentration-dependent manner. Exposure to nano Cu 
      increased the formation of acidic vesicular organelles and the expression of 
      Beclin-1, microtubule-associated protein 1 light chain 3, and p62, and treatment 
      with an autophagy inhibitor reduced nephrotoxicity. This indicated that the 
      autophagy pathway is involved in the toxicity induced by nanosized copper 
      particles to mesangial cells. This finding can contribute to the development of 
      safety guidelines for the evaluation of nanomaterials in the future.
FAU - Xu, Pengjuan
AU  - Xu P
AUID- ORCID: 0000-0002-4320-3006
AD  - School of Integrative Medicine, Tianjin University of Traditional Chinese 
      Medicine, Tianjin, China.
FAU - Cao, Min
AU  - Cao M
AUID- ORCID: 0000-0003-3010-1980
AD  - School of Integrative Medicine, Tianjin University of Traditional Chinese 
      Medicine, Tianjin, China.
FAU - Dong, Xueqian
AU  - Dong X
AUID- ORCID: 0000-0002-0957-8584
AD  - School of Integrative Medicine, Tianjin University of Traditional Chinese 
      Medicine, Tianjin, China.
FAU - Yu, Zhichao
AU  - Yu Z
AUID- ORCID: 0000-0001-9676-1063
AD  - Scientific Research Office, Tianjin Sino-German University of Applied Sciences, 
      Tianjin, China.
FAU - Liu, Jianwei
AU  - Liu J
AUID- ORCID: 0000-0001-7120-2951
AD  - School of Integrative Medicine, Tianjin University of Traditional Chinese 
      Medicine, Tianjin, China.
FAU - Tan, Junzhen
AU  - Tan J
AUID- ORCID: 0000-0003-2953-6004
AD  - School of Integrative Medicine, Tianjin University of Traditional Chinese 
      Medicine, Tianjin, China.
FAU - Wang, Yiyi
AU  - Wang Y
AUID- ORCID: 0000-0003-2346-3887
AD  - Department of Neurology, Tianjin Haihe Hospital, Tianjin, China.
FAU - Li, Tao
AU  - Li T
AUID- ORCID: 0000-0001-8820-6350
AD  - School of Integrative Medicine, Tianjin University of Traditional Chinese 
      Medicine, Tianjin, China.
FAU - Zhao, Shuwu
AU  - Zhao S
AUID- ORCID: 0000-0001-5754-0532
AD  - School of Integrative Medicine, Tianjin University of Traditional Chinese 
      Medicine, Tianjin, China.
LA  - eng
PT  - Journal Article
DEP - 20221111
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - EC 3.4.22.- (Caspase 3)
RN  - 789U1901C5 (Copper)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Beclin-1)
RN  - 0 (Microtubule-Associated Proteins)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Caspase 3
MH  - *Copper/toxicity/metabolism
MH  - *Mesangial Cells/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Beclin-1/metabolism
MH  - Autophagy
MH  - Apoptosis
MH  - Microtubule-Associated Proteins/metabolism
MH  - Cell Line, Tumor
PMC - PMC9668079
EDAT- 2022/11/17 06:00
MHDA- 2022/11/19 06:00
PMCR- 2022/11/11
CRDT- 2022/11/16 15:53
PHST- 2022/08/09 00:00 [received]
PHST- 2022/10/06 00:00 [accepted]
PHST- 2022/11/16 15:53 [entrez]
PHST- 2022/11/17 06:00 [pubmed]
PHST- 2022/11/19 06:00 [medline]
PHST- 2022/11/11 00:00 [pmc-release]
AID - S0100-879X2022000100674 [pii]
AID - 10.1590/1414-431X2022e12252 [doi]
PST - epublish
SO  - Braz J Med Biol Res. 2022 Nov 11;55:e12252. doi: 10.1590/1414-431X2022e12252. 
      eCollection 2022.