PMID- 36385489
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20230201
IS  - 1478-3231 (Electronic)
IS  - 1478-3223 (Linking)
VI  - 43
IP  - 2
DP  - 2023 Feb
TI  - LINC01608 activated by YY1 facilitate hepatocellular carcinoma progression by 
      modulating the EGFR/ERK axis.
PG  - 471-489
LID - 10.1111/liv.15479 [doi]
AB  - BACKGROUND: Long non-coding RNAs (LncRNAs) have been demonstrated to associate 
      with a variety of cancers. However, the mechanisms of LncRNAs in hepatocellular 
      carcinoma (HCC) progression are still not fully clarified. METHODS: LINC01608 
      expression level in HCC and adjacent normal tissues was detected by 
      real-time-quantitively PCR (RT-qPCR) in clinical samples and in situ 
      hybridization (ISH) in tissue microarray. Several functional assays were 
      performed to determine the biological effects of LINC01608 in HCC cells in vitro, 
      while subcutaneous xenograft models and lung metastasis models in nude mice and 
      immunohistochemistry (IHC) results showed the role of LINC01608 in HCC 
      progression in vivo. The combination of LINC01608 with miR-875-5p and target 
      genes was elucidated by dual-luciferase report assays, RNA immunoprecipitation 
      (RIP) assays and fluorescence in situ hybridization (FISH) assays. Finally, 
      bioinformatics analysis and chromatin immunoprecipitation (CHIP) were performed 
      to investigate the mechanism of Yin Yang-1 (YY1) regulating LINC01608 
      transcription. RESULTS: LINC01608 was overexpressed in HCC tissues, and high 
      LINC01608 expression predicted poor overall survival (OS) and disease-free 
      survival (DFS) in HCC patients. LINC01608 could promote HCC cell proliferation, 
      migration, invasion and epithelial-mesenchymal transition (EMT) in vitro and in 
      vivo. Furthermore, we demonstrated that LINC01608 could sponge to miR-875-5p and 
      activate the EGFR/ERK pathway. Moreover, we identified transcriptional factor YY1 
      could bind to the promoter of LINC01608 and induce its transcription. CONCLUSION: 
      LINC01608 could serve as a promising prognostic biomarker of HCC. YY1-activated 
      LINC01608 could promote HCC progression by associating with miR-875-5p to induce 
      the EGFR/ERK signalling pathway. This discovery might provide therapeutic 
      strategies for HCC.
CI  - (c) 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Han, Mengzhen
AU  - Han M
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Liu, Furong
AU  - Liu F
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Li, Xinxin
AU  - Li X
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Zhang, Hongwei
AU  - Zhang H
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Pan, Yonglong
AU  - Pan Y
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Liu, Yachong
AU  - Liu Y
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Zhu, He
AU  - Zhu H
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Liang, Huifang
AU  - Liang H
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Chen, Xiaoping
AU  - Chen X
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Liao, Zhibin
AU  - Liao Z
AUID- ORCID: 0000-0003-0305-2079
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Zhang, Zhanguo
AU  - Zhang Z
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
FAU - Zhang, Bixiang
AU  - Zhang B
AD  - Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
AD  - Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, Hubei, China.
LA  - eng
GR  - 82172976/National Natural Science Foundation of China/
GR  - 81502530/National Natural Science Foundation of China/
GR  - 81874189/National Natural Science Foundation of China/
GR  - 82103597/National Natural Science Foundation of China/
GR  - [2010] 493-51/the Chinese Ministry of Public Health for Key Clinical Project/
GR  - 2018ZX10723204-003/the State Key Project on Infection Disease of China/
PT  - Journal Article
DEP - 20221125
PL  - United States
TA  - Liver Int
JT  - Liver international : official journal of the International Association for the 
      Study of the Liver
JID - 101160857
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - 0 (YY1 protein, human)
RN  - 0 (YY1 Transcription Factor)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - 0 (MIRN-875 microRNA, human)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Humans
MH  - *Carcinoma, Hepatocellular/pathology
MH  - *Liver Neoplasms/pathology
MH  - *MicroRNAs/genetics
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Mice, Nude
MH  - In Situ Hybridization, Fluorescence
MH  - Cell Line, Tumor
MH  - ErbB Receptors/genetics
MH  - YY1 Transcription Factor/genetics/therapeutic use
OTO - NOTNLM
OT  - EGFR
OT  - EMT
OT  - YY1
OT  - biomarker
OT  - hepatocellular carcinoma
OT  - long non-coding RNA
EDAT- 2022/11/18 06:00
MHDA- 2023/01/25 06:00
CRDT- 2022/11/17 11:09
PHST- 2022/11/08 00:00 [revised]
PHST- 2022/06/25 00:00 [received]
PHST- 2022/11/12 00:00 [accepted]
PHST- 2022/11/18 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2022/11/17 11:09 [entrez]
AID - 10.1111/liv.15479 [doi]
PST - ppublish
SO  - Liver Int. 2023 Feb;43(2):471-489. doi: 10.1111/liv.15479. Epub 2022 Nov 25.