PMID- 36388798
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221119
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 10
IP  - 20
DP  - 2022 Oct
TI  - Sodium Danshensu protects against oxygen glucose 
      deprivation/reoxygenation-induced astrocytes injury through regulating NOD-like 
      receptor pyrin domain containing 3 (NLRP3) inflammasome and tuberous sclerosis 
      complex-2 (TSC2)/mammalian target of rapamycin (mTOR) pathways.
PG  - 1097
LID - 10.21037/atm-22-2143 [doi]
LID - 1097
AB  - BACKGROUND: Cerebral ischemic stroke is a serious condition with high incidence, 
      mortality, and associated disability. Currently, effective therapeutic options 
      are available for ischemic stroke are limited. Accumulating evidence indicates 
      that sodium Danshensu, mono sodium compound derived from Salvia miltiorrhiza, 
      plays protective roles in ischemic stroke. However, the underlying protective 
      mechanism of sodium Danshensu in cerebral ischemic stroke remains unknown. 
      METHODS: In the current study, we explored the role and mechanism of sodium 
      Danshensu on astrocytes exposed to oxygen-glucose deprivation/reoxygenation 
      (OGD/R), which mimics the process of ischemia-reperfusion. The impact of sodium 
      Danshensu on cell viability and apoptosis after OGD/R were evaluated by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-dophenyl tetrazolium bromide (MTT) assay and 
      flow cytometry. Quantitative reverse transcription polymerase chain reaction 
      (qRT-PCR) and western blot were used to detect the expression of target messenger 
      RNA (mRNA) and proteins associated with apoptosis and autophagy. The release of 
      lactate dehydrogenase (LDH) was determined, and the production of proinflammatory 
      cytokines were detected using enzyme-linked immunosorbent assay (ELISA) kits. 
      RESULTS: It was found that sodium Danshensu could significantly increase cell 
      viability and decrease LDH release and apoptosis. Besides, it inhibited the 
      production of proinflammatory cytokines, including tumor necrosis factor-alpha 
      (TNF-alpha), interleukin (IL)-1beta, and IL-6. Sodium Danshensu also dose-dependently 
      decreased protein and mRNA levels of nucleotide binding oligomerization NOD-like 
      receptor pyrin domain containing 3 (NLRP3) and high mobility group box 1 (HMGB1), 
      which play a crucial role in promoting ischemic stroke-induced cell injury. 
      Moreover, sodium Danshensu dose-dependently upregulated Beclin 1 expression, 
      downregulated P62 protein expression, and further increased LC3B-II/LC3B-I ratio 
      through inducing autophagy in astrocytes. Additionally, we noticed that sodium 
      Danshensu dose-dependently increased tuberous sclerosis complex-2 (TSC2) protein 
      expression, while significantly reduced the levels of mammalian target of 
      rapamycin (mTOR) in the presence of OGD/R insult. CONCLUSIONS: These findings 
      suggest that sodium Danshensu protects against OGD/R-induced injury by modulating 
      the NLRP3 inflammasome and TSC2/mTOR pathways.
CI  - 2022 Annals of Translational Medicine. All rights reserved.
FAU - Hu, Shengzhao
AU  - Hu S
AD  - Department of Emergency, the First Affiliated Hospital of Nanchang University, 
      Nanchang, China.
FAU - Chen, Yingli
AU  - Chen Y
AD  - Department of Hematology, Jiangxi Provincial Children's Hospital, Nanchang, 
      China.
FAU - Huang, Shipeng
AU  - Huang S
AD  - Department of Emergency, the First Affiliated Hospital of Nanchang University, 
      Nanchang, China.
FAU - Liu, Min
AU  - Liu M
AD  - Department of Emergency, the First Affiliated Hospital of Nanchang University, 
      Nanchang, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Department of Emergency, the First Affiliated Hospital of Nanchang University, 
      Nanchang, China.
FAU - Huang, Shaofang
AU  - Huang S
AD  - Department of Emergency, the First Affiliated Hospital of Nanchang University, 
      Nanchang, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC9652549
OTO - NOTNLM
OT  - Autophagy
OT  - NOD-like receptor pyrin domain containing 3 inflammasome (NLRP3 inflammasome)
OT  - ischemic stroke
OT  - sodium Danshensu
OT  - tuberous sclerosis complex-2/mammalian target of rapamycin (TSC2/mTOR)
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at 
      https://atm.amegroups.com/article/view/10.21037/atm-22-2143/coif). The authors 
      have no conflicts of interest to declare.
EDAT- 2022/11/18 06:00
MHDA- 2022/11/18 06:01
PMCR- 2022/10/01
CRDT- 2022/11/17 12:17
PHST- 2022/03/28 00:00 [received]
PHST- 2022/08/31 00:00 [accepted]
PHST- 2022/11/17 12:17 [entrez]
PHST- 2022/11/18 06:00 [pubmed]
PHST- 2022/11/18 06:01 [medline]
PHST- 2022/10/01 00:00 [pmc-release]
AID - atm-10-20-1097 [pii]
AID - 10.21037/atm-22-2143 [doi]
PST - ppublish
SO  - Ann Transl Med. 2022 Oct;10(20):1097. doi: 10.21037/atm-22-2143.