PMID- 36398610
OWN - NLM
STAT- MEDLINE
DCOM- 20221201
LR  - 20221201
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 70
IP  - 47
DP  - 2022 Nov 30
TI  - Relationships of Matrix Metalloproteinase 1 and a Tissue Inhibitor of 
      Metalloproteinase to Collagen Metabolism in Haliotis discus hannai.
PG  - 14886-14897
LID - 10.1021/acs.jafc.2c05931 [doi]
AB  - In response to physical, chemical, and/or biological stimuli, considerable tissue 
      self-degradation occurs in abalone, causing severe post-harvest quality loss. 
      During this process, the extracellular matrix (ECM) is greatly degraded by 
      endogenous proteases. The main component of the ECM is collagen, primarily type I 
      collagen. Although the activity of matrix metalloproteinases (MMPs), which can 
      specifically degrade collagen, is precisely regulated by tissue inhibitors of MPs 
      (TIMPs), indicating that MMPs and TIMPs play crucial roles in the regulation of 
      tissue self-degradation, few studies have reported the interaction between MMPs 
      and TIMPs. In this study, we reveal collagenases to participate in postmortem 
      tissue self-degradation of Haliotis discus hannai by degrading type I collagen. 
      The recombinant MMP-1 catalytic domain (rMMP1c) of abalone with high purity and 
      enzyme activity is expressed using a prokaryotic expression system. The optimum 
      temperature and pH for rMMP1c are 37  degrees C and 7.0, respectively. The thermal 
      denaturation temperature of rMMP1c is 67.0 +/- 0.9  degrees C. Ethylenediamine tetraacetic 
      acid (EDTA) and 1,10-phenanthroline can completely inhibit rMMP1c activity, while 
      Ba(2+), Ca(2+), and Mg(2+) can significantly elevate it. TIMP is also expressed 
      using HEK 293F cells. Recombinant TIMP (rTIMP) shows good inhibitory activity 
      toward rMMP1c. Inhibition kinetics analyses reveal rTIMP to be a competitive 
      inhibitor of rMMP1c. Biolayer interferometry reveals that rTIMP can effectively 
      bind with rMMP1c, with an equilibrium dissociation constant value of 263 nM. 
      rMMP1c effectively degrades type I collagen gamma-beta-alpha chains in turn, and rTIMP can 
      significantly inhibit rMMP1c degradation activity. These results provide a 
      theoretical basis for the study of MMP and TIMP interaction and elucidate the 
      possible mechanism for abalone tissue self-degradation.
FAU - Chen, Yu-Lei
AU  - Chen YL
AUID- ORCID: 0000-0003-2893-6540
AD  - College of Ocean Food and Biological Engineering, Jimei University, Xiamen 
      361021, China.
AD  - Collaborative Innovation Center of Marine Food Deep Processing, Dalian 
      Polytechnic University, Dalian 116034, China.
FAU - Zhang, Ming-Hui
AU  - Zhang MH
AD  - College of Ocean Food and Biological Engineering, Jimei University, Xiamen 
      361021, China.
FAU - Su, Le-Le
AU  - Su LL
AD  - College of Ocean Food and Biological Engineering, Jimei University, Xiamen 
      361021, China.
FAU - Sun, Le-Chang
AU  - Sun LC
AD  - College of Ocean Food and Biological Engineering, Jimei University, Xiamen 
      361021, China.
AD  - Collaborative Innovation Center of Marine Food Deep Processing, Dalian 
      Polytechnic University, Dalian 116034, China.
FAU - Qiu, Xu-Jian
AU  - Qiu XJ
AD  - College of Ocean Food and Biological Engineering, Jimei University, Xiamen 
      361021, China.
FAU - Lin, Duanquan
AU  - Lin D
AD  - College of Ocean Food and Biological Engineering, Jimei University, Xiamen 
      361021, China.
AD  - Collaborative Innovation Center of Marine Food Deep Processing, Dalian 
      Polytechnic University, Dalian 116034, China.
FAU - Zhang, Ling-Jing
AU  - Zhang LJ
AD  - College of Ocean Food and Biological Engineering, Jimei University, Xiamen 
      361021, China.
AD  - Collaborative Innovation Center of Marine Food Deep Processing, Dalian 
      Polytechnic University, Dalian 116034, China.
FAU - Jin, Tengchuan
AU  - Jin T
AUID- ORCID: 0000-0002-1395-188X
AD  - CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life 
      Sciences and Medicine, University of Science & Technology of China, Hefei 230007, 
      China.
FAU - Cao, Min-Jie
AU  - Cao MJ
AD  - College of Ocean Food and Biological Engineering, Jimei University, Xiamen 
      361021, China.
AD  - Collaborative Innovation Center of Marine Food Deep Processing, Dalian 
      Polytechnic University, Dalian 116034, China.
LA  - eng
PT  - Journal Article
DEP - 20221118
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
RN  - 0 (Collagen Type I)
RN  - EC 3.4.- (Metalloproteases)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
SB  - IM
MH  - Animals
MH  - *Matrix Metalloproteinase 1/genetics
MH  - Collagen Type I/genetics
MH  - Metalloproteases
MH  - *Gastropoda/genetics
MH  - Tissue Inhibitor of Metalloproteinases
OTO - NOTNLM
OT  - Haliotis discus hannai
OT  - characterization
OT  - degradation
OT  - matrix metalloproteinase 1
OT  - tissue inhibitor of metalloproteinase
OT  - type I collagen
EDAT- 2022/11/19 06:00
MHDA- 2022/12/02 06:00
CRDT- 2022/11/18 04:44
PHST- 2022/11/19 06:00 [pubmed]
PHST- 2022/12/02 06:00 [medline]
PHST- 2022/11/18 04:44 [entrez]
AID - 10.1021/acs.jafc.2c05931 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2022 Nov 30;70(47):14886-14897. doi: 10.1021/acs.jafc.2c05931. 
      Epub 2022 Nov 18.