PMID- 36398670
OWN - NLM
STAT- MEDLINE
DCOM- 20221121
LR  - 20221121
IS  - 1828-1427 (Electronic)
IS  - 0505-401X (Linking)
VI  - 58
IP  - 1
DP  - 2022 Nov 18
TI  - Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the 
      porcine reproductive and respiratory syndrome virus.
PG  - 47-55
LID - 10.12834/VetIt.2182.14564.1 [doi]
AB  - In this study, lung tissue was collected from nursery piglets suspected of being 
      infected with porcine reproductive and respiratory syndrome virus (PRRSV) in a 
      large‑scale pig farm in Sichuan, China. Polymerase chain reaction (PCR) and 
      reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) methods 
      were used to ensure that no other pathogens were present. Virus isolation was 
      also carried out where the presence of PRRSV was determined by indirect 
      immunoinfluscent assay (IFA). Compared with the common PRRSV strain, the isolate 
      did not produce evident Cytopathic effect (CPE) in the early stage of isolation. 
      CPE was found in the late stage, and the titer was 104.17 TCID50/0.1 mL. The 
      strain was named CJS01. Bioinformatics analysis showed that it was a NADC30‑Like 
      strain. The virus load was determined by measuring the nucleic acid load during 
      the proliferation of the strain on Marc‑145 cells. The strain showed good 
      adaptability on cells, and the virus proliferated on cells for 84 hr when the 
      highest nucleic acid load was achieved. By recombinant analysis of ORF3~7 genes 
      and prediction of its epitope, it was found that CJS01 strain might interfere 
      with the immunesystem of the infected animals.
FAU - Tu, Teng
AU  - Tu T
AD  - .College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 
      611130, Sichuan Province, China. 309349398@qq.com.
FAU - Wang, Yin
AU  - Wang Y
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, 
      Sichuan Province, China.
FAU - Liao, Chang Yu
AU  - Liao CY
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, 
      Sichuan Province, China.
FAU - Zhang, Peng Fei
AU  - Zhang PF
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, 
      Sichuan Province, China.
FAU - Xiang, Ming Yuan
AU  - Xiang MY
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, 
      Sichuan Province, China.
FAU - Yang, Ze Xiao
AU  - Yang ZX
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, 
      Sichuan Province, China.
FAU - Yao, Xue Ping
AU  - Yao XP
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, 
      Sichuan Province, China.
FAU - Luo, Yan
AU  - Luo Y
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, 
      Sichuan Province, China.
LA  - eng
PT  - Journal Article
DEP - 20221118
PL  - Italy
TA  - Vet Ital
JT  - Veterinaria italiana
JID - 0201543
RN  - 0 (Nucleic Acids)
SB  - IM
MH  - Animals
MH  - Swine
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - Computational Biology
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - *Nucleic Acids
MH  - *Swine Diseases
EDAT- 2022/11/19 06:00
MHDA- 2022/11/22 06:00
CRDT- 2022/11/18 05:06
PHST- 2020/02/14 00:00 [received]
PHST- 2021/01/26 00:00 [accepted]
PHST- 2022/11/18 05:06 [entrez]
PHST- 2022/11/19 06:00 [pubmed]
PHST- 2022/11/22 06:00 [medline]
AID - 10.12834/VetIt.2182.14564.1 [doi]
PST - epublish
SO  - Vet Ital. 2022 Nov 18;58(1):47-55. doi: 10.12834/VetIt.2182.14564.1.